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PB_tre_dCas9_KRAB

Plasmid

#126030

Purpose

PiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusion

Depositor

Mauro Calabrese

Article

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Inserts

dCas9 fusion with KRAB
Hygromycin resistance

Use

CRISPR; Piggybac

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

EF1 alpha and TRE

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam p53 R175H

Plasmid

#16436

Depositor

Bert Vogelstein

Article

Baker et al Science. 1990 Aug 24. 249(4971):912-5.

Insert

p53 R175H (TP53 Human)

Expression

Mammalian

Mutation

R175H

Promoter

CMV

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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KG371-GFP-mios

Plasmid

#87052

Purpose

mammalian expression of mios

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

mios (MIOS Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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AAV_CAG_V5-LOV-Turbo-NES

Plasmid

#199670

Purpose

expresses V5-LOV-Turbo-NES in mammalian cells, AAV vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

AAV

Tags

NES and V5

Promoter

CAG

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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AnkyrinG-190-GFP

Plasmid

#31059

Depositor

Vann Bennett

Article

Mohler et al J Biol Chem. 2002 Mar 22. 277(12):10599-

Insert

Ankyrin G (Ank3 Rat)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 28, 2012

Availability

Academic Institutions and Nonprofits only

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tdTomato-KIF1A

Plasmid

#102850

Purpose

Overexpresses KIF1A and cell fill TdTomato

Depositor

Erik Dent

Article

McVicker et al Nat Commun. 2016 Sep 23;7:12741. doi: 10

Insert

KIF1A (Kif1a Rat)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pXJ-His-CD276

Plasmid

#232662

Purpose

transient expresses CD276 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

CD276 (CD276 Human)

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam p53 R273H

Plasmid

#16439

Depositor

Bert Vogelstein

Article

Baker et al Science. 1990 Aug 24. 249(4971):912-5.

Insert

p53 R273H (TP53 Human)

Expression

Mammalian

Mutation

R273H

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam p53 R248W

Plasmid

#16437

Depositor

Bert Vogelstein

Article

Baker et al Science. 1990 Aug 24. 249(4971):912-5.

Insert

p53 R248W (TP53 Human)

Expression

Mammalian

Mutation

R248W

Available Since

Aug. 4, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-Puro_siKD

Plasmid

#86695

Purpose

Tetracycline inducible expression of shRNA targeted to AAVS1 locus

Depositor

Ludovic Vallier

Article

Bertero et al Development. 2016 Dec 1;143(23):4405-441

Type

Empty backbone

Expression

Mammalian

Promoter

H1 TO

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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Oct4-IRES-eGFP-PGK-Neo

Plasmid

#48681

Purpose

For knock-in donor vector by CRISPR/CAS system

Depositor

Rudolf Jaenisch

Article

Yang et al Cell. 2013 Aug 27. pii: S0092-8674(13)01

Insert

Oct4 (Pou5f1 Mouse)

Use

CRISPR

Tags

GFP

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-SFXN1

Plasmid

#110634

Purpose

Retroviral expression of Flag-SFXN1 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

SFXN1 (SFXN1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized cDNA (no amino acid mutations)

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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PB_tre_dCas9_VP160

Plasmid

#126031

Purpose

PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

Depositor

Mauro Calabrese

Article

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Inserts

dCas9 fusion with VP160
Hygromycin resistance

Use

CRISPR; Piggybac

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

EF1 alpha and TRE

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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PGK-H2BeGFP

Plasmid

#21210

Depositor

Mark Mercola

Article

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

Phosphoglycerate kinase promoter (PGK1 Human)

Use

Lentiviral

Tags

H2B-eGFP

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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pmCherry-ATG5

Plasmid

#13095

Depositor

Roberta Gottlieb

Article

Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

Insert

ATG5 (Atg5 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pHTNW

Plasmid

#136403

Purpose

Expression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Type

Empty backbone

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-mGFP-SNX9

Plasmid

#223632

Purpose

Lentiviral expression of human SNX9 with mGFP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SNX9 (SNX9 Human)

Use

Lentiviral; mammalian expression

Tags

mGFP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pT077

Plasmid

#137879

Purpose

Donor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFP

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA

Use

AAV

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam p53 R249S

Plasmid

#16438

Depositor

Bert Vogelstein

Article

Baker et al Science. 1990 Aug 24. 249(4971):912-5.

Insert

p53 R249S (TP53 Human)

Expression

Mammalian

Mutation

R249S

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

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lenti SYN-dCas9-KRAB-MeCP2

Plasmid

#155365

Purpose

Expresses dCas9-KRAB-MeCP2 fusion driven by human SYN promoter

Depositor

Jeremy Day

Article

Duke et al Front Genome Ed. 2020 Sep 15;2:9. doi: 1

Insert

dCas9-KRAB-MeCP2

Use

CRISPR and Lentiviral

Tags

3x FLAG and 7x His

Expression

Mammalian

Promoter

SYN

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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