Addgene

We narrowed to 16,452 results for: EMB

Showing: 6921 - 6940 of 16452 results

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6xHis(TEV)-Drp1-StrepII

Plasmid

#174422

Purpose

Expresses human Drp1 Isoform 3 in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Use

Tags

6xHis(TEV) and StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis(TEV)-Drp1(K38A)-StrepII

Plasmid

#174423

Purpose

Expresses human Drp1 Isoform 3 with K38A mutation in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Use

Tags

6xHis(TEV) and StrepII

Expression

Bacterial

Mutation

K38A

Promoter

T7

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P28

Plasmid

#120039

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xHA

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xHA

Use

Pcr template

Tags

3xHA

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Nanog5P reporter

Plasmid

#16337

Depositor

Austin Cooney

Article

Gu et al Mol Cell Biol. 2005 Oct . 25(19):8507-19

Insert

Nanog promoter (Nanog Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Mouse Nanog promoter

Available since

Dec. 6, 2007

Availability

Academic Institutions and Nonprofits only

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GFP-cortactin

Plasmid

#26722

Depositor

Anna Huttenlocher

Article

Perrin et al Mol Biol Cell. 2006 Jan . 17(1):239-50.

Insert

Cortactin (Cttn Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 30, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3-eNOS-GFP

Plasmid

#22444

Depositor

William Sessa

Article

Sowa et al J Biol Chem. 1999 Aug 6. 274(32):22524-3

Insert

WT eNOS-GFP

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_193 NRL + TetO

Plasmid

#157812

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with central TetO in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_tetO

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-Gsdmd-NT 4A

Plasmid

#80952

Purpose

Expresses Gsdmd-NT 4A mutant in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-NT 4A (Gsdmd Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pNES-iRFP-C1

Plasmid

#116863

Purpose

cytosolic iRFP

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

iRFP713

Use

Tags

X.leavis map2k1.L(32-44)

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pSicoR-mCh-empty

Plasmid

#21907

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.

Depositor

Miguel Ramalho-Santos

Article

Gaspar-Maia et al Nature. 2009 Jul 8. ():.

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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Lenti_AID-Dam-lamin B1

Plasmid

#119764

Purpose

Lentiviral plasmid for Mammalian Expression

Depositor

Maria-Elena Torres-Padilla

Article

Borsos et al Nature. 2019 May;569(7758):729-733. doi:

Insert

AID-Dam-lamin B1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g4

Plasmid

#80787

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 4/14/24 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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T7-RelA(K310R)

Plasmid

#23250

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

NFkB RelA (RELA Human)

Use

Tags

T7 gp10

Expression

Mammalian

Mutation

K310R

Promoter

Available since

March 15, 2010

Availability

Academic Institutions and Nonprofits only
pLJM1 RagA

Plasmid

#73031

Purpose

Expresses human RagA from a CMV promoter for stable expression in mammalian cells

Depositor

David Sabatini

Article

Efeyan et al Dev Cell. 2014 May 12;29(3):321-9. doi:

Insert

rragA (RRAGA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCX-Klf5

Plasmid

#66657

Purpose

For expression of mouse Klf5.

Depositor

Barak Cohen

Article

Fiore et al Genome Res. 2016 Jun;26(6):778-86. doi:

Insert

Klf5 (Klf5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g1

Plasmid

#80784

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 1/11/21 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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p006-GFP-Logic-AND

Plasmid

#112237

Purpose

GFP from jellyfish Aquorea sp. under D61 AND gate-like ara+lac promoter with AraC repressor; lacI repressor; kanamycin resistant.

Depositor

Jaroslaw Bryk

Article

Siddall et al bioRxiv 2021.06.20.449138

Inserts

GFP under control of doubly-repressible promoter
GFP
araC

Use

Tags

Expression

Bacterial

Mutation

Promoter

AND-gate and pLacI

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt2

Plasmid

#86593

Purpose

Expresses eGFP-tagged human Akt2(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2 (AKT2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g2

Plasmid

#80785

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 2/12/22 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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SB_convex

Plasmid

#110104

Purpose

pBXPHM3 with scaffold Sybody of the convex library

Depositor

Markus Seeger

Article

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Insert

scaffold Sybody of the convex library

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

May 24, 2018

Availability

Academic Institutions and Nonprofits only

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