We narrowed to 26,296 results for: Vit

1. p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A

   Plasmid

   #83484

   Purpose

   Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lbu_C2c2_R1048A_H1053A
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   R1048A, H1053A

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHisAlix

   Plasmid

   #42576

   Depositor

   James Hurley

   Article

   Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

   Insert

   ALIX (PDCD6IP Human)
   

   Tags

   HIS-TEV

   Expression

   Bacterial

   Mutation

   silent mutation A1617G (numbering in ALIX gene)

   Available Since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pMAL-PUM2

   Plasmid

   #120385

   Purpose

   Bacterial expression of MBP-Pumillio2 fusion protein

   Depositor

   Igor Ulitsky

   Article

   Tichon et al Nat Commun. 2016 Jul 13;7:12209. doi: 10

   Insert

   PUM2 (PUM2 Human)
   

   Tags

   6xHIS and MBP

   Expression

   Bacterial

   Promoter

   LacI

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-hSyn-Gαi*-BERKY3

   Plasmid

   #158428

   Purpose

   BRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSyn

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   hSynapsin

   Available Since

   Sept. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. All-in-one split-ADAR2

   Plasmid

   #170136

   Purpose

   AAV vector carrying ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-FKBP-mTagBFP2-SOScat

   Plasmid

   #178866

   Purpose

   Mammalian expression of SOS1(Rem-Cdc25) fused to FKBP-mTagBFP2

   Depositor

   Shinya Tsukiji

   Article

   Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

   Insert

   FKBP-mTagBFP2-SOS(Rem-Cdc25) (SOS1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. piGECI-NES-N1

   Plasmid

   #160422

   Purpose

   Expression of a near-infrared genetically encoded calcium indicator iGECI with a nuclear export signal sequence

   Depositor

   Vladislav Verkhusha

   Article

   Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

   Insert

   iGECI-NES
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac(His10-SNAP-Csk)

   Plasmid

   #177895

   Purpose

   Baculo expression of His10 tag fused to SNAPtag and human Csk

   Depositor

   Ron Vale

   Article

   Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

   Insert

   Csk (CSK Human)
   

   Tags

   10xHis-TEV-TVMV-SNAP tag

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBEST_OR2-OR1-Pr-UTR1_ClpP-T500

   Plasmid

   #153302

   Purpose

   Expresses ClpP protease in the cell-free TX-TL system

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

   Insert

   clpP (clpP Escherichia coli)
   

   Expression

   Bacterial

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. RapR-Shp2-mVenus-flag

    Plasmid

    #188655

    Purpose

    Rapamycin regulated Shp2 phosphatase with mVenus and flag tag

    Depositor

    Andrei Karginov

    Article

    Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

    Insert

    Shp2
    

    Tags

    Flag and mVenus

    Expression

    Mammalian

    Mutation

    iFKBP insertion at V406 with deletion of residues…

    Promoter

    CMV

    Available Since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-h-mmPtch1-FL-TagBFP

    Plasmid

    #120921

    Purpose

    Expresses BFP tagged Ptch1

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    ptch1 (Ptch1 Mouse)
    

    Tags

    TagBFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV Syn ChR E90R-T159C 2A tDimer

    Plasmid

    #52495

    Purpose

    Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

    Depositor

    Thomas Oertner

    Article

    Wietek et al Science. 2014 Mar 27.

    Inserts

    Channelrhodopsin-2
    red fluorescent protein
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    E90R, T159C

    Promoter

    human synapsin-1 and ribosomal skip sequence

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221_SLC22A3

    Plasmid

    #132024

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

    Insert

    SLC22A3 (SLC22A3 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. XE241 Rat frizzled-1-GFP-CS2P+

    Plasmid

    #16817

    Depositor

    Randall Moon

    Article

    Kaykas et al Nat Cell Biol. 2004 Jan . 6(1):52-8.

    Insert

    frizzled-1 (Fzd1 Rat)
    

    Use

    Xenopus expression

    Tags

    GFP

    Available Since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pCMV-HA-mIFITM3-C71,72,105A

    Plasmid

    #58396

    Purpose

    Expressed murine IFITM3-C71,72,105A with an N-terminal HA tag in mammalian cells

    Depositor

    Howard Hang, Jacob Yount

    Article

    Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

    Insert

    IFITM3 (Ifitm3 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Cysteines 71, 72, and 105 to Alanine

    Promoter

    CMV IE

    Available Since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pegRNA_HEK4_20-7

    Plasmid

    #201974

    Purpose

    Express a pegRNA used for 20bp tag insertion at HEK4 site with a 7bp homology arm

    Depositor

    Scot Wolfe

    Article

    Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

    Insert

    pegRNA_HEK4_20-7
    

    Expression

    Mammalian

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSD117A

    Plasmid

    #218141

    Purpose

    Expresses AviTag-6xHis-MBP-TEV-SD40 for protein purification from E. coli

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    SD40
    

    Tags

    AviTag-6xHis-MBP-TEV-(GGGGS)2

    Available Since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. 645 pCS2-etv2

    Plasmid

    #49005

    Purpose

    Mammalian Expression vector, In-situ probe template and in vitro transcription vector for zebrafish Etv2

    Depositor

    Nathan Lawson

    Article

    Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

    Insert

    ets variant gene 2 (etsrp Zebrafish)
    

    Use

    Invitro transcription, riboprobe template

    Expression

    Mammalian

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pME DN-E-cadherin

    Plasmid

    #135209

    Purpose

    Dominate Negative zebrafish e-cadherin label

    Depositor

    David Tobin

    Article

    Cronan et al Immunity. 2016 Oct 18;45(4):861-876. doi

    Insert

    CDH1
    

    Use

    Synthetic Biology

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. XE49 pt Beta-catenin-myc

    Plasmid

    #16840

    Depositor

    Randall Moon

    Article

    Yost et al Genes Dev. 1996 Jun 15. 10(12):1443-54.

    Insert

    pt beta-catenin-myc (ctnnb1.L Frog)
    

    Use

    Xenopus expression

    Tags

    6x myc

    Mutation

    four point mutations introduced into the GSK-3 ph…

    Available Since

    June 25, 2009

    Availability

    Academic Institutions and Nonprofits only