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JWW-1 human chimeric antibody

Plasmid

#66748

Purpose

Expresses a rat/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 17-36. JWW-1 works in ELISA/WB/HPV Neutralization assay.

Depositor

Richard Roden

Article

Wang et al Clin Vaccine Immunol. 2015 May 13. pii:

Inserts

JWW-1 Heavy Chain Gamma
JWW-1 Light Chain Kappa

Use

Tags

Expression

Mammalian

Mutation

First 3 amino acids of rat light chain constant r…

Promoter

mEF1 and rEF1

Available since

July 24, 2015

Availability

Academic Institutions and Nonprofits only

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VAMP2-SNAP

Plasmid

#105288

Purpose

VAMP2 tagged with SNAP

Depositor

Justin Taraska

Article

Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

Insert

VAMP2-SNAP (Vamp2 Rat)

Use

Tags

SNAP tag

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Grin1 KI #3

Plasmid

#139659

Purpose

Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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YPet-Lysosomes-20

Plasmid

#56636

Purpose

Localization: Lysosome Membrane, Excitation: 513, Emission: 527

Depositor

Michael Davidson

Insert

LAMP1

Use

Tags

YPet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2014

Availability

Industry, Academic Institutions, and Nonprofits
SECURE BE3(R33A)-P2A-EGFP (pJUL1085)

Plasmid

#123614

Purpose

CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A).

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

BE3(R33A)-P2A-EGFP

Use

Tags

Expression

Mammalian

Mutation

R33A in rAPOBEC1, D10A in SpCas9

Promoter

CAG

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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miniSOG-RatLC3

Plasmid

#57775

Purpose

Localization: Microtubule-Binding Protein, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

LC3

Use

Tags

miniSOG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
Cherry-HA-KCC2

Plasmid

#104077

Purpose

To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)

Depositor

Igor Medyna

Article

Kahle et al EMBO Rep. 2014 Jul;15(7):766-74. doi: 10

Insert

KCC2 (b isoform) (Slc12a5 Rat)

Use

Tags

mCherry-HA tag

Expression

Mammalian

Mutation

EcoR1 site in rat KCC2 was removed by silent muta…

Promoter

UbC

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMK284 (Stag-3FLAG-Hygro)

Plasmid

#72800

Purpose

C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

Stag-3FLAG-Hygro

Use

CRISPR

Tags

Stag-3FLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 - PP1g - Flag - BirA* (AA2-140)

Plasmid

#86886

Purpose

N-terminal part of the split-BioID

Depositor

Mathieu Bollen

Article

Munter et al FEBS Lett. 2016 Dec 29. doi: 10.1002/187

Insert

PP1g - FLAG - BirA (AA2-140)

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

BirA Amino Acids 2-140

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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RFP Dynamin2 Wild Type

Plasmid

#128152

Purpose

Expression of Dynamin 2

Depositor

Jennifer Lippincott-Schwartz

Article

Chua et al Proc Natl Acad Sci U S A. 2009 Nov 30. (

Insert

Dynamin 2 (Dnm2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV5-rat ERK2-WT

Plasmid

#40812

Depositor

Natalie Ahn

Article

Emrick et al Proc Natl Acad Sci U S A. 2006 Nov 28;10

Insert

MAPK1 (Mapk1 Rat)

Use

Tags

His6

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
PKC beta I CAT

Plasmid

#16380

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC beta 1 (Prkcb Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

CAT: catalytic domain, aa 329-671

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
CMV::ratSyGCaMP2

Plasmid

#26124

Depositor

Leon Lagnado

Article

Dreosti et al Nat Methods. 2009 Dec . 6(12):883-9.

Insert

Synaptophysin GCaMP2 (Syp Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 11, 2010

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1 WT IRES GFP

Plasmid

#133816

Purpose

Encodes full-length wild type synaptotagmin 1 for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Expression

Mutation

Promoter

hSyn (human synapsin I promoter)

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Arpc5 KI

Plasmid

#131503

Purpose

Endogenous tagging of Arp2/3 complex subunit 5: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Arpc5, Arpc5 Rat, Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pHLSec2-irisin-his

Plasmid

#122729

Purpose

Expresses in mammalian cells (HEK293):glycosylated and secreted irisin-his

Depositor

Harold Erickson

Article

Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

Insert

irisin (ectodomain of FNDC5) (Fndc5 Rat, Mouse, Human, Bovine)

Use

Tags

his and signal sequence for secretion

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pT3-EFa1-HA-Jag1

Plasmid

#46051

Depositor

Xin Chen

Article

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Jag1 (Jag1 Rat)

Use

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA HisB TO rFez1

Plasmid

#17385

Depositor

Stephen Goff

Article

Naghavi et al Genes Dev. 2005 May 1. 19(9):1105-15.

Insert

FEZ1 (Fez1 Rat)

Use

Tags

his and myc

Expression

Mammalian

Mutation

Promoter

Available since

March 1, 2010

Availability

Academic Institutions and Nonprofits only
GFP-C1-CAMKIIalpha-T286A

Plasmid

#21220

Depositor

Tobias Meyer

Article

Shen et al Science. 1999 Apr 2. 284(5411):162-6.

Insert

CAMKIIalpha-T286A (Camk2a Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

T286A (Lab plasmid KS 0003).

Promoter

Available since

July 29, 2009

Availability

Academic Institutions and Nonprofits only
Halo-p58

Plasmid

#166896

Purpose

mammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with HaloTag

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

p58 (Lman1 Rat)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

L405Q, R419S- please see depositor comments

Promoter

CMV

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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