Addgene

We narrowed to 2,096 results for: AP1

Showing: 1701 - 1750 of 2096 results

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pCMV-Flag-YAP-S127/387A

Plasmid

#33079

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S127A, S387A

Promoter

CMV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA4/HisMaxB-YAP2-1st WW mutant

Plasmid

#18998

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Jul 17. ():.

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

His

Expression

Mammalian

Mutation

Tryptophan 199 changed to Alanine, and Proline 2…

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA4/HisMaxB-YAP2-2nd WW mutant

Plasmid

#18999

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Jul 17. ():.

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

His

Expression

Mammalian

Mutation

Tryptophan 258 changed to Alanine, and Proline 2…

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pET3a pro-hRegIIIa

Plasmid

#64935

Purpose

bacterial expression of full length human RegIIIalpha

Depositor

Lora Hooper

Article

Cash et al Protein Expr Purif. 2006 Jul;48(1):151-9

Insert

RegIIIa (REG3A Human)

Use

Tags

Expression

Bacterial

Mutation

mutations made in 5' end to relax secondary …

Promoter

T7

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
pET-NEMOL329P

Plasmid

#11969

Depositor

Jon Ashwell

Article

Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

Insert

NEMOL329P (IKBKG Human)

Use

Tags

His

Expression

Bacterial

Mutation

Leucine329 to Proline

Promoter

Available since

July 13, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA4/HisMaxB-YAP2-deltaC

Plasmid

#21124

Depositor

Marius Sudol

Article

Tsutomu Oka & Marius et al

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

His

Expression

Mammalian

Mutation

5 Carboxy-terminal amino acids -FLTWL are deleted

Promoter

Available since

July 1, 2009

Availability

Academic Institutions and Nonprofits only
pDest17_THRAP3_THSD7A fusion protein LCD (663-955)

Plasmid

#235002

Purpose

Bacterial expression of N-terminally 6His tagged THRAP3_THSD7A (THRAP3) fusion protein LCD

Depositor

Tanja Mittag

Insert

THRAP3_THSD7A (THRAP3) (Thyroid hormone receptor-associated protein) (THRAP3, THSD7A Human)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pZH81 pET21b Rat1-His

Plasmid

#231650

Purpose

Bacterial expression vector expressing yeast S. cerevisiae rat1 with a C-terminal his tag

Depositor

Jesper Svejstrup

Article

Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

Insert

Rat1 (RAT1 Budding Yeast)

Use

Tags

6His

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD02_CBASS_Pseudoalteromonas_sp._A2

Plasmid

#224401

Purpose

For expression of the type I CBASS system encoded by Pseudoalteromonas sp. A2

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

Psp.CdnB_AGS-C_Cap15

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD02_CBASS_Pectobacterium_parmentieri

Plasmid

#224400

Purpose

For expression of the type I CBASS system encoded by Pectobacterium parmentieri

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

PpCdnB_AGS-C_Cap15

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Alteromonas_sp._ALT199

Plasmid

#224399

Purpose

For expression of the type I CBASS system encoded by Alteromonas sp. ALT199

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

Asp.CdnB_AGS-C_Cap15

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPM-miR30-MOV10-ts1

Plasmid

#174277

Purpose

MOV10 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MOV10 shRNA (MOV10 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPM-miR30-MOV10-ts2

Plasmid

#174278

Purpose

MOV10 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MOV10 shRNA (MOV10 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPM-miR30-MOV10-ts3

Plasmid

#174279

Purpose

MOV10 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MOV10 shRNA (MOV10 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET24-aCatenin(82-906)-His6

Plasmid

#203332

Purpose

Bacterial expression of wild-type alpha-catenin

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2012 May 25;287(22):18492-9

Insert

CTNNA1 (CTNNA1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

aa 82-906

Promoter

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGBT9-γ1 ear

Plasmid

#199177

Purpose

Expression of GAL4 DNA-binding domain (BD)-AP-1 γ1 ear domain fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

Insert

AP-1 γ1 ear domain (Ap1g1 Mouse)

Use

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Mutation

Promoter

ADH1

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-γ2 ear

Plasmid

#199178

Purpose

Expression of GAL4 DNA-binding domain (BD)-AP-1 γ2 ear domain fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

Insert

AP-1 γ2 ear domain (AP1G2 Human)

Use

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Mutation

Promoter

ADH1

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
MAC_N_ELK4

Plasmid

#187745

Purpose

MAC-tagged gene expression of human ELK4

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

ELK4 (ELK4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0CM0061

Plasmid

#177026

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

iridoid oxidase; CYP76A26

Use

Synthetic Biology

Tags

Expression

Mutation

amplified from cDNA, contains T359S relative to K…

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0CM0818

Plasmid

#177020

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

geranyl pyrophosphate synthase; geranyl diphosphate synthase

Use

Synthetic Biology

Tags

Expression

Mutation

transit peptide removed

Promoter

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_HSPA8-Halo

Plasmid

#175325

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HSPA8 (HSPA8 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-FLAG-PGRMC-Hybrid1

Plasmid

#173469

Purpose

N-terminal region of PGRMC2 fused to the transmembrane and C-terminal region of PGRMC1

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC1/PGRMC2 fusion (PGRMC1, PGRMC2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-FLAG-PGRMC-Hybrid2

Plasmid

#173470

Purpose

N-terminal and transmembrane region of PGRMC2 fused to the C-terminal region of PGRMC1

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC1/PGRMC2 fusion (PGRMC1, PGRMC2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-FLAG-PGRMC-Hybrid3

Plasmid

#173471

Purpose

N-terminal region of PGRMC1 fused to the transmembrane and C-terminal region of PGRMC2

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC1/PGRMC2 fusion (PGRMC1, PGRMC2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_SRGAP3-1/1

Plasmid

#91510

Purpose

Protein expression and purification of human SH3 domain construct SRGAP3-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SRGAP3-1/1 (SRGAP3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_SRGAP3-1/1

Plasmid

#91390

Purpose

Protein expression and purification of human SH3 domain construct SRGAP3-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SRGAP3-1/1 (SRGAP3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-hPLEKHM1

Plasmid

#64145

Purpose

For yeast two hybrid

Depositor

Tamotsu Yoshimori

Article

Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

Insert

PLEKHM1 (PLEKHM1 Human)

Use

Tags

GAL4 activation domain

Expression

Yeast

Mutation

Promoter

ADH

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-S127A/G385V

Plasmid

#33072

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S127A, G385V

Promoter

CMV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-S127/D383A

Plasmid

#33078

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S127A, D383A

Promoter

CMV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-DDB1

Plasmid

#19918

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

DDB1 (Damage-specific DNA binding protein 1) (DDB1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
6599-SUMO1GG

Plasmid

#13377

Depositor

Anne Dejean

Article

Seeler et al Mol Cell Biol. 2001 May . 21(10):3314-24

Insert

SUMO1 (SUMO1 Human)

Use

Tags

Expression

Mammalian

Mutation

mature form ending with GG

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19/human IL-8

Plasmid

#17610

Depositor

Joost Oppenheim

Article

Matsushima et al J Exp Med. 1988 Jun 1. 167(6):1883-93.

Insert

IL8 (CXCL8 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA2-DDB1

Plasmid

#19909

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

Damage-specific DNA binding protein 1 (DDB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

please see depositor comment below

Promoter

Available since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
OMM-V5-LOV-Turbo

Plasmid

#199665

Purpose

expresses LOV-Turbo on the mammalian outer mitochondrial membrane, lentiviral vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

Lentiviral

Tags

AKAP1 transmembrane domain and V5

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-6XHis-human SUMO 1

Plasmid

#133770

Purpose

express 6Xhis- SUMO 1 protein

Depositor

Martine Roussel

Article

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 1 (SUMO1 Human)

Use

Tags

6XHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1-hs-ALPS 1-ALPS2-EGFP

Plasmid

#187113

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSG00000101199 (ARFGAP1 Human)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 HA-Sumo1 WT

Plasmid

#48966

Purpose

Expresses HA-Sumo1 WT in mammalian cells

Depositor

Guy Salvesen

Article

Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

Insert

SUMO1 (SUMO1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

T7

Available since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pQTEV-ANP32A

Plasmid

#31563

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

acidic (leucine-rich) nuclear phosphoprotein 32 family, member A (ANP32A Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2-AN

Plasmid

#20950

Depositor

William Kaelin

Article

Neuman et al Mol Cell Biol. 1994 Oct . 14(10):6607-15

Insert

E2F-1 promoter, genomic fragment (E2F1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

wt human E2F-1 promoter sequence

Promoter

Available since

Aug. 10, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pMGIB-3xFLAG-dyskerin

Plasmid

#53607

Purpose

Retroviral expression of human dyskerin

Depositor

Steven Artandi

Article

Venteicher et al Science. 2009 Jan 30;323(5914):644-8. do

Insert

dyskerin (DKC1 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

LTR

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pWPI-E2F1

Plasmid

#114296

Purpose

Constitutive expression of the E2F1 protein

Depositor

Patrick Salmon

Article

Rady et al Cell Cycle. 2013 Aug 15;12(16):2691-702.

Insert

E2F1 (E2F1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Daxx/pEGFP

Plasmid

#119021

Purpose

Express GFP-Daxx in mamalian cells

Depositor

Xiaolu Yang

Article

Tang et al PLoS One. 2013;8(2):e55813. doi: 10.1371

Insert

death-domain associated protein (Daxx) (DAXX Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pmcherry α-catenin WT

Plasmid

#178646

Purpose

express wild-type α-catenin

Depositor

Daniel Conway

Article

Bejar-Padilla et al Mol Biol Cell. 2022 Aug 3:mbcE22020071.

Insert

α-catenin (CTNNA1 Human)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-HA-ITFG2

Plasmid

#87045

Purpose

mammalian expression of ITFG2

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

ITFG2 (ITFG2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
σ2-mCherry

Plasmid

#186579

Purpose

Expression of adaptor protein 2 (AP2) sigma subunit AP2S1 tagged with mCherry

Depositor

Stephen Royle

Article

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Insert

AP2S1 (AP2S1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO GFP HSPA8

Plasmid

#19487

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

HSPA8 (HSPA8 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
h-Plekhm1_EGFP

Plasmid

#73836

Purpose

Express human Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

Insert

Plekhm1 (PLEKHM1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO HSPA8

Plasmid

#19460

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

HSPA8 (HSPA8 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
6598-HisSUMO1GG

Plasmid

#13376

Depositor

Anne Dejean

Article

Seeler et al Mol Cell Biol. 2001 May . 21(10):3314-24

Insert

SUMO1 (SUMO1 Human)

Use

T7 in vitro translation

Tags

His

Expression

Mammalian

Mutation

mature form ending with GG

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 HA-Sumo1 Q94P

Plasmid

#48965

Purpose

Expresses HA SUMO1 Q94P mutant in mammalian cells

Depositor

Guy Salvesen

Article

Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

Insert

SUMO1 (SUMO1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Mutated Q94P

Promoter

T7

Available since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only

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