We narrowed to 2,903 results for: BFP

1. pMN-015

   Plasmid

   #195419

   Purpose

   AAVS1 Integratable mCherry Reporter for synZiFTR ZF7

   Depositor

   Ahmad Khalil

   Article

   Li et al Science. 2022 Dec 16;378(6625):1227-1234

   Insert

   4X ZF7 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
   

   Expression

   Mammalian

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMN-016

   Plasmid

   #195420

   Purpose

   AAVS1 Integratable mCherry Reporter for synZiFTR ZF8

   Depositor

   Ahmad Khalil

   Article

   Li et al Science. 2022 Dec 16;378(6625):1227-1234

   Insert

   4X ZF8 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
   

   Expression

   Mammalian

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMN-017

   Plasmid

   #195421

   Purpose

   AAVS1 Integratable mCherry Reporter for synZiFTR ZF9

   Depositor

   Ahmad Khalil

   Article

   Li et al Science. 2022 Dec 16;378(6625):1227-1234

   Insert

   4X ZF9 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
   

   Expression

   Mammalian

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMN-019

   Plasmid

   #195423

   Purpose

   AAVS1 Integratable mCherry Reporter for synZiFTR ZF11

   Depositor

   Ahmad Khalil

   Article

   Li et al Science. 2022 Dec 16;378(6625):1227-1234

   Insert

   4X ZF11 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
   

   Expression

   Mammalian

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCVL SFFV Sbf-Sal linker

   Plasmid

   #50434

   Purpose

   Cloning vector for coexpression of gene cloned in between Sbf and Sal sites with BFP in mammalian cells. Made for cloning of meganuclease for direct comparison with megaTALs (pCVL TAL Xba-Sal).

   Depositor

   Andrew Scharenberg

   Article

   Boissel et al Nucleic Acids Res. 2013 Nov 26.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   May 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pU6Rosa-CAG-Gal4-Rad18UBD

   Plasmid

   #119012

   Purpose

   Mammalian expression of Rosa26 sgRNA, fusion protein of yeast Gal4 with human Rad18 Ubiquitin binding domain and BFP expression

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   Gal4 (GAL4 Budding Yeast)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. InterTag-2xL

   Plasmid

   #186912

   Purpose

   Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a (GGGGS)2 linker and the other side expressing BFP and puromycin resistance gene

   Depositor

   Allen Liu, Taekjip Ha

   Article

   Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

   Insert

   SpyTag003-sfCherry11-(GGGGS)2-PDGFR
   

   Expression

   Mammalian

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. InterTag-1xL

   Plasmid

   #186911

   Purpose

   Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a single GGGGS linker and the other side expressing BFP and puromycin resistance gene

   Depositor

   Allen Liu, Taekjip Ha

   Article

   Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

   Insert

   SpyTag003-sfCherry11-(GGGGS)1-PDGFR
   

   Expression

   Mammalian

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-Gal4-RNF169UBD

   Plasmid

   #119013

   Purpose

   Mammalian expression of fusion protein of yeast Gal4 with human RNF169 Ubiquitin binding domain and BFP expression

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   Gal4 (GAL4 Budding Yeast)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSpCas9_T2A_mCherry sg1-sg2

    Plasmid

    #192479

    Purpose

    All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Tags

    T2A mCherry

    Expression

    Mammalian

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCRISPRia-v2

    Plasmid

    #84832

    Purpose

    CRISPRi/a V2 library parental plasmid

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Inserts

    sgRNA
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAG-BRCA1

    Plasmid

    #119007

    Purpose

    Mammalian expression of human BRCA1 and BFP

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    BRCA1 (BRCA1 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. sg2.02 16x(MS2) MUC4.1

    Plasmid

    #101154

    Purpose

    gRNA with 16 MCP binding site

    Depositor

    Mazhar Adli

    Article

    Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

    Insert

    MUC4 (MUC4 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TetO-FUW-VdC9BV

    Plasmid

    #62195

    Purpose

    Expresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous Genes

    Depositor

    Kam Leong

    Article

    Chakraborty et al Stem Cell Reports. 2014 Dec 9;3(6):940-7

    Insert

    VP64dCas9BFPVP64
    

    Use

    Lentiviral

    Tags

    Two VP64s tagged to dCas9 fused to BFP

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive) Cas9 (dCas9)

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. sg14x(MS2) MUC4.1

    Plasmid

    #101153

    Purpose

    gRNA with 14 MCP binding site

    Depositor

    Mazhar Adli

    Article

    Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

    Insert

    MUC4 (MUC4 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Dox-Akt

    Plasmid

    #207328

    Purpose

    PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized Akt1 (without pleckstrin homology domain)

    Depositor

    Jared Toettcher

    Article

    Underhill et al Development. 2023 Aug 15;150(16):dev2016

    Insert

    rac protein kinase-alpha (AKT1 Human)
    

    Tags

    myristoylation tag - BFP

    Expression

    Bacterial

    Mutation

    range: 129 - 480

    Promoter

    Tet Response Element

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. sg2.02 MUC4.1

    Plasmid

    #101152

    Purpose

    gRNA with two MCP binding sites

    Depositor

    Mazhar Adli

    Article

    Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

    Insert

    MUC4 (MUC4 Human)
    

    Use

    CRISPR and Lentiviral

    Promoter

    mU6

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLG1-puro-sgATL3-2

    Plasmid

    #109010

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL3-sgRNA #2 (ATL3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    mU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMK1224

    Plasmid

    #84221

    Purpose

    Lentiviral expression of shRNAs, compatible with double-shRNA expression, SFFV promoter, BFP

    Depositor

    Martin Kampmann, Jonathan Weissman

    Article

    Kampmann et al Proc Natl Acad Sci U S A. 2015 Jun 30;11

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLG1-puro-sgATL3-5

    Plasmid

    #109011

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL3-sgRNA #5 (ATL3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    mU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pk335-NT-1mer-gRNA

    Plasmid

    #227500

    Purpose

    non-targeting control guide

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    Non-targeting gRNA
    

    Use

    CRISPR

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEIF3D-WT-sgNT

    Plasmid

    #222120

    Purpose

    EIF3D overexpression vector with non-targeting sgRNA

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    eIF3D (EIF3D Human)
    

    Use

    Lentiviral

    Tags

    BFP-P2A

    Available Since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 probasin_mTQ2_FlpO

    Plasmid

    #68356

    Purpose

    -The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and SMAD4 ex2. are expressed by the U6 promoter

    Depositor

    Jannik Elverløv-Jakobsen

    Inserts

    4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7
    FlpO recombinase
    

    Use

    CRISPR

    Tags

    mTurquoise2 (BFP)

    Expression

    Mammalian

    Promoter

    Synthetic Probasin ARRx2 and U6

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMK1334

    Plasmid

    #127965

    Purpose

    sgRNA expression vector compatible with CROP-Seq and imaging screens

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    EF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Aug. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHRm-NLS-dCas9-GFP11x7-NLS

    Plasmid

    #70224

    Purpose

    Expresses NLS-dCas9-GFP11x7-NLS in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    NLS-dCas9-GFP11x7-NLS
    

    Tags

    GFP11x7

    Expression

    Mammalian

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGMC00029 (aka pRC0610)

    Plasmid

    #200834

    Purpose

    Lentiviral guide RNA expression plasmid for CRISPR activation with puromycin/BFP

    Depositor

    Raj Chari

    Article

    Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAN2981

    Plasmid

    #127246

    Purpose

    Asymmetric degronSwitchA_t8 fused to mCherry with KeyA-BFP

    Depositor

    Hana El-Samad

    Article

    Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

    Insert

    pPGK-Key_A_e18-TagBFP-P2A-mCherry-asym_degronSwitch_A_t8-WPRE
    

    Use

    Lentiviral and Synthetic Biology

    Available Since

    June 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLG1-puro-sgULK1-1

    Plasmid

    #109004

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ULK1-sgRNA #1 (ULK1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    mU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAN1579

    Plasmid

    #127201

    Purpose

    Progesterone inducible expression of KeyC-BFP

    Depositor

    Hana El-Samad

    Article

    Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

    Insert

    pPAB1-Z3PM-tTDH1-pZ3-key_C-mTagBFP2-tENO1
    

    Use

    Synthetic Biology

    Available Since

    May 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLG1-puro-sgATL2-1

    Plasmid

    #109008

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL2-sgRNA #1 (ATL2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    mU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAN1572

    Plasmid

    #127202

    Purpose

    Dual inducible expression of Venus-degronSwitchA and KeyA-BFP

    Depositor

    Hana El-Samad

    Article

    Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

    Insert

    pGal1-Venus-degronSwitch_A_t12-tSSA1-pZ3-key_A_e18-mTagBFP2-tENO1
    

    Use

    Synthetic Biology

    Available Since

    Aug. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLG1-puro-sgATL2-2

    Plasmid

    #109009

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL2-sgRNA #2 (ATL2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    mU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pEIF3D-RNAgateDel-sgEIF3D

    Plasmid

    #222137

    Purpose

    EIF3D variant overexpression vector with sgRNA targeting eIF3D

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    eIF3D (EIF3D Human)
    

    Use

    Lentiviral

    Tags

    BFP-P2A

    Mutation

    V291-F306 deletion

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEIF3D-S528D-S529D-sgEIF3D

    Plasmid

    #222127

    Purpose

    EIF3D variant overexpression vector with sgRNA targeting eIF3D

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    eIF3D (EIF3D Human)
    

    Use

    Lentiviral

    Tags

    BFP-P2A

    Mutation

    S528D, S529D

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEIF3D-NtermDel-CtermDel-sgEIF3D

    Plasmid

    #222131

    Purpose

    EIF3D variant overexpression vector with sgRNA targeting eIF3D

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    eIF3D (EIF3D Human)
    

    Use

    Lentiviral

    Tags

    BFP-P2A

    Mutation

    161 + 527 truncation

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEIF3D-NtermDel-sgEIF3D

    Plasmid

    #222123

    Purpose

    EIF3D variant overexpression vector with sgRNA targeting eIF3D

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    eIF3D (EIF3D Human)
    

    Use

    Lentiviral

    Tags

    BFP-P2A

    Mutation

    S161 truncation

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEIF3D-a11helixDel-sgEIF3D

    Plasmid

    #222135

    Purpose

    EIF3D variant overexpression vector with sgRNA targeting eIF3D

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    eIF3D (EIF3D Human)
    

    Use

    Lentiviral

    Tags

    BFP-P2A

    Mutation

    T317E, N320E, H321A

    Available Since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEIF3D-CtermDel-sgEIF3D

    Plasmid

    #222125

    Purpose

    EIF3D variant overexpression vector with sgRNA targeting eIF3D

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    eIF3D (EIF3D Human)
    

    Use

    Lentiviral

    Tags

    BFP-P2A

    Mutation

    F527 truncation

    Available Since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEIF3D-a5helixDel-sgEIF3D

    Plasmid

    #222133

    Purpose

    EIF3D variant overexpression vector with sgRNA targeting eIF3D

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    eIF3D (EIF3D Human)
    

    Use

    Lentiviral

    Tags

    BFP-P2A

    Mutation

    D249Q, V262I, Y263A

    Available Since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEIF3D-S528N-S529N-sgEIF3D

    Plasmid

    #222129

    Purpose

    EIF3D variant overexpression vector with sgRNA targeting eIF3D

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    eIF3D (EIF3D Human)
    

    Use

    Lentiviral

    Tags

    BFP-P2A

    Mutation

    S528N, S529N

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCAG-BRCA1-RNF169UBD

    Plasmid

    #119009

    Purpose

    Mammalian expression of a fusion protein of human BRCA1 with the human RNF169 Ubiquitin binding domain and BFP expression

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    BRCA1 (BRCA1 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCAG-BRCA1-Rad18UBD

    Plasmid

    #119008

    Purpose

    Mammalian expression of a fusion protein of human BRCA1 with human Rad18 Ubiquitin binding domain and BFP expression

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    BRCA1 (BRCA1 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. MTK7_010

    Plasmid

    #123877

    Purpose

    Encodes promoter-less tagBFP T2A Blasticidin resistance cassette to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    BFP-T2A-Blasticidin-BgPa
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pAN1578

    Plasmid

    #127200

    Purpose

    Progesterone inducible expression of KeyA-BFP

    Depositor

    Hana El-Samad

    Article

    Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

    Insert

    pPAB1-Z3PM-tTDH1-pZ3-key_A_e18-mTagBFP2-tENO1
    

    Use

    Synthetic Biology

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB

    Plasmid

    #154473

    Purpose

    Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ZIM3 (ZIM3 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Includes ZIM3 aa 1-100

    Promoter

    SFFV

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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46. pBA439

    Plasmid

    #85967

    Purpose

    Perturb-seq vector backbone

    Depositor

    Jonathan Weissman

    Article

    Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

    Inserts

    sgGFP-NT2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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47. pSLQ10622

    Plasmid

    #239274

    Purpose

    Expresses PYL1-KIFC1* for the retrograde transport of RNA along microtubules via CRISPR-TO

    Depositor

    Stanley Qi

    Article

    Han et al Nature. 2025 Jul;643(8070):241-251. doi:

    Insert

    PYL1-BFP-KIFC1(aa125-673) (KIFC1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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48. CAG-mCherry-sesRNA-GFP-WPRE-pA

    Plasmid

    #192065

    Purpose

    Expression of mCherry, sesRNA in mammalian cells, with GFP as efRNA.

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    sesRNA for Cheta
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. pk335-CARGO-6mer-70kb-DSF

    Plasmid

    #227498

    Purpose

    6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to 70kb Downstream Fgf5
    

    Use

    CRISPR

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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50. pNW538_pAAVS1-pAP1(2)-FlpO-ABI-P2A-PYL-FlpO-CAG-U1-frt-STOP-frt-U2-GFP-P2A-luc2-ZeoR

    Plasmid

    #192939

    Purpose

    FlpO-based digitizer circuit driven by AP1 promoter

    Depositor

    Wilson Wong

    Article

    Wong et al Nat Commun. 2021 Oct 22;12(1):6150. doi:

    Inserts

    AP1(2)/minTK promoter
    FlpO-ABI
    PYL-FlpO
    CAG promoter
    frt-STOP-frt
    GFP
    luciferase
    pPGK
    Zeocin resistance
    BFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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