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Showing: 1701 - 1750 of 6863 results

  3. PtetA-PBAD-PLacO3O1 200bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid
    #225649
    Purpose
    tetA, araBAD and LacO3O1 promoters in tandem conformation with 200bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance
    Depositor
    Andre Sanches Ribeiro
    Article
    Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/
    Insert
    tetR/tetA, araBAD and LacO3O1 in tandem conformation
    Use
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    tetA, araBAD and LacO3O1 promoters
    Available since
    Oct. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    225649-plasmid-map-sequence-id-448716

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  4. AAV-hSyn-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

    Plasmid
    #177360
    Purpose
    AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression from Synapsin promoter
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Inserts
    tdTomato
    Chimeric guide for SaCas9
    Use
    AAV
    Tags
    P2A and a tetracycline-controlled transactivator …
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsine promoter and U6 promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177360-plasmid-map-sequence-id-353669

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  5. AAV-CMV-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

    Plasmid
    #177365
    Purpose
    AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Inserts
    tdTomato
    Chimeric guide for SaCas9
    Use
    AAV
    Tags
    P2A and a tetracycline-controlled transactivator …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6 promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177365-plasmid-map-sequence-id-353664

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  9. Tet-inducible 3xFlag Mouse C325A Caspase-9 ΔCARD

    Plasmid
    #194608
    Purpose
    Inducible Expression of N-Terminal 3X Flag Mouse C325A Caspase-9 Mutant Minus CARD Domain
    Depositor
    Hannah Rabinowich
    Article
    Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649
    Insert
    N-Terminal 3X Flag Mouse C325A Caspase-9 Mutant Minus Sequence Encoding Met 1 to Lys 176 (Casp9 Mouse)
    Use
    Tags
    3X Flag
    Expression
    Mammalian
    Mutation
    Deletion of N-terminal CARD domain and changed Cy…
    Promoter
    CMV
    Available since
    Feb. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    194608-plasmid-map-sequence-id-384891

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  12. KAN(G418)-TET-OFF – ubiquitin – LEU – 4MYC

    Plasmid
    #193318
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    Ubiquitin - Leucine - 4MYC N-terminal tagging
    Use
    Tags
    4MYC
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    193318-plasmid-map-sequence-id-383635

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  13. KAN(G418)-TET-OFF – ubiquitin – LEU – 4FLAG

    Plasmid
    #193317
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    Ubiquitin - Leucine - 4FLAG N-terminal tagging
    Use
    Tags
    4FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    193317-plasmid-map-sequence-id-383630

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  14. pSEVA251::Ptrc.x.tetO2::B0032::CYP110D1 N-terminal His-Tag

    Plasmid
    #186710
    Purpose
    N-terminal His-tagged CYP110D1 coding sequence under the control of Ptrc.x.tetO2 promoter and the BBa_B0032 RBS, for the expression in Synechocystis.
    Depositor
    Paula Tamagnini
    Article
    Mascia et al Green Chemistry, 24 (2022):6156
    Insert
    alr4766
    Use
    Synthetic Biology
    Tags
    His-Tag (6His)
    Expression
    Mutation
    Promoter
    Ptrc.x.tetO2
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    186710-plasmid-map-sequence-id-368444

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  16. TetO-FUW-FLAG-NLS-EnR-MCS_N-term

    Plasmid
    #136884
    Purpose
    Doxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)
    Depositor
    Moritz Mall
    Article
    Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi
    Insert
    engrailed repressor domain (en Fly)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    136884-plasmid-map-sequence-id-272359

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  20. KA901: pMVP (L3-L2) HA tag + pA; EF1a::eGFP-P2A-TETa-pA

    Plasmid
    #121804
    Purpose
    pMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven GFP-P2A-TETa
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    HA epitope tag-polyA + EF1a::eGFP-P2A-TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121804-plasmid-map-sequence-id-237313

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  21. KA701: pMVP (L3-L2) HA tag + pA; RIP::eGFP-P2A-TETa-pA

    Plasmid
    #121808
    Purpose
    pMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven GFP-P2A-TETa
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    HA epitope tag-polyA + RIP::eGFP-P2A-TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121808-plasmid-map-sequence-id-237328

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  33. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

    Plasmid
    #183411
    Purpose
    piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    Tet-on 3G
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1A
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183411-plasmid-map-sequence-id-361257

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  36. AAVS1-TetOn-3XFLAG-firefly-beta-globin-PTC39-AAVS1

    Plasmid
    #184397
    Purpose
    Donor plasmid for stable integration of firefly luciferase NMD(+) PTC39 reporter at AAVS1
    Depositor
    Robert Bradley
    Article
    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:
    Insert
    Firefly luciferase beta-globin-PTC39
    Use
    CRISPR; Donor plasmid for hdr
    Tags
    3X FLAG
    Expression
    Mammalian
    Mutation
    PTC in beta-globin at amino acid position 39
    Promoter
    Tet-On 3G
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    184397-plasmid-map-sequence-id-362358

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  37. AAVS1-TetOn-3XFLAG-renilla-beta-globin-PTC39-AAVS1

    Plasmid
    #184398
    Purpose
    Donor plasmid for stable integration of Renilla luciferase NMD(+) PTC39 reporter at AAVS1
    Depositor
    Robert Bradley
    Article
    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:
    Insert
    Renilla luciferase beta-globin-PTC39
    Use
    CRISPR; Donor plasmid for hdr
    Tags
    3X FLAG
    Expression
    Mammalian
    Mutation
    PTC in beta-globin at amino acid position 39
    Promoter
    Tet-On 3G
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    184398-plasmid-map-sequence-id-362369

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  40. tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid
    #69546
    Purpose
    Tet-On system expressing MyoD-2A-GFP-2xNLS.
    Depositor
    Charles Gersbach
    Inserts
    eGFP
    Myod1 (Myod1 Mouse)
    Use
    Lentiviral
    Tags
    2x nuclear localization sequence and T2A "sk…
    Expression
    Mutation
    Removed stop codon to fuse it to the 2A-eGFPnls i…
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69546-plasmid-map-sequence-id-122992

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  42. PhiC31-Neo-ins-5xTetO-pEF-H2B-Citrine-ins

    Plasmid
    #78099
    Purpose
    Nuclear fluorescent reporter in mammalian cells, phiC31 attB for integration, can be silenced with TetR-CR or rTetR-CR (e.g. CR can be KRAB or DNMT3B).
    Depositor
    Michael Elowitz
    Article
    Bintu et al Science. 2016 Feb 12;351(6274):720-4. do
    Insert
    Histone H2B - Citrine (H2BC21 Synthetic, Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    5xTetO pEF alpha
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only

  43. pRRL-pEF-H2B-mCherry-T2A-rTetR-Dest-SV40

    Plasmid
    #186968
    Purpose
    Lentiviral destination vector for cloning desired protein as rTetR fusion for chromatin regulation reporter (pEF-H2B-mCherry-T2A-rTetR, with SV40) for expression in mammalian cells.
    Depositor
    Brian Liau
    Article
    Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d
    Insert
    pEF-H2B-mCherry-T2A-rTetR-Dest-SV40
    Use
    Lentiviral
    Tags
    rTetR fusion
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1alpha promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    186968-plasmid-map-sequence-id-370475

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Showing: 1701 - 1750 of 6863 results