We narrowed to 6,927 results for: crispr cas9 plasmids

1. pAAV-FLEX-SaCas9-U6-sgGabrg2

   Plasmid

   #124857

   Purpose

   Mutagenesis of Gabrg2

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gabrg2 (Gabrg2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEN-dCas9-CDA1-UGI

   Plasmid

   #196248

   Purpose

   Plasmid containing promoter of RecA from Xanthomonas oryzae driving expression of dead Cas9, cytosine deaminase CDA1, and glycosylase inhibitor

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Insert

   attL4-RecApro-dCas9-CDA1-UGI-attR1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   RecA promoter

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-FLEX-SaCas9-U6-sgGria2

   Plasmid

   #124854

   Purpose

   Mutagenesis of Gria2

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gria2 (Gria2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a6

   Plasmid

   #124860

   Purpose

   Mutagenesis of Slc17a6

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc17a6 (Slc17a6 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-FLEX-SaCas9-U6-sgGrin2a

   Plasmid

   #124850

   Purpose

   Mutagenesis of Grin2a

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Grin2a (Grin2a Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-FLEXfrt-SaCas9-U6-sgGria2

   Plasmid

   #124868

   Purpose

   Mutagenesis of Gria2

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gria2 (Gria2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-FLEXfrt-SaCas9-U6-sgGrin1

   Plasmid

   #124865

   Purpose

   Mutagenesis of Grin1

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Grin1 (Grin1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-FLEXfrt-SaCas9-U6-sgGabrg1

   Plasmid

   #124870

   Purpose

   Mutagenesis of Gabrg1

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gabrg1 (Gabrg1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-FLEX-SaCas9-U6-sgGabrg1

   Plasmid

   #124856

   Purpose

   Mutagenesis of Gabrg1

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gabrg1 (Gabrg1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-FLEXfrt-SaCas9-U6-sgGrin2b

    Plasmid

    #124864

    Purpose

    Mutagenesis of Grin2b

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Grin2b (Grin2b Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-FLEX-SaCas9-U6-sgGabrg3

    Plasmid

    #124858

    Purpose

    Mutagenesis of Gabrg3

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gabrg3 (Gabrg3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-FLEXfrt-SaCas9-U6-sgGrin2a

    Plasmid

    #124863

    Purpose

    Mutagenesis of Grin2a

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Grin2a (Grin2a Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-FLEXfrt-SaCas9-U6-sgGabrg3

    Plasmid

    #124872

    Purpose

    Mutagenesis of Gabrg3

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gabrg3 (Gabrg3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-FLEXfrt-SaCas9-U6-sgGabrg2

    Plasmid

    #124871

    Purpose

    Mutagenesis of Gabrg2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gabrg2 (Gabrg2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

    Plasmid

    #183409

    Purpose

    piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells

    Depositor

    Azim Surani

    Article

    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

    Insert

    dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Tol2_B-act_dCas9-Vp64-T2A-Citrine

    Plasmid

    #200248

    Purpose

    Zebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesis

    Depositor

    Tatjana Sauka-Spengler

    Article

    Pelea et al bioRxiv 2023.05.08.539738

    Insert

    dCas9-Vp64-T2A-Citrine
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. eSpCas9(1.1)-Puro

    Plasmid

    #117686

    Purpose

    The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.

    Depositor

    Luc Leyns

    Article

    Liu et al Biochem Biophys Res Commun. 2019 Feb 14.

    Insert

    eSpCas9(1.1)
    

    Use

    CRISPR

    Tags

    PuroR (R166H)

    Expression

    Mammalian

    Mutation

    K848A, K1003A, R1060A

    Promoter

    U6

    Available since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZS157 CRISPEY RT/Cas9

    Plasmid

    #114454

    Purpose

    Yeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expression

    Depositor

    Hunter Fraser

    Article

    Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

    Insert

    SpCas9, Ec86-RT
    

    Use

    CRISPR

    Tags

    SV40-NLS

    Expression

    Yeast

    Mutation

    S. cerevisiae codon optimized

    Promoter

    GAL1-GAL10

    Available since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-T7-TadCBEd-SpCas9-P2A-EGFP (BKS327)

    Plasmid

    #223123

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd-SpCas9 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadCBEd-SpCas9-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpCas9=D10A

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001)

    Plasmid

    #174091

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV and T7

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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