We narrowed to 9,240 results for: pho

1. AAV hSyn hPGK1-mRuby

   Plasmid

   #220911

   Purpose

   Neuronal specific expression of the fusion construct PGK1-mRuby

   Depositor

   Timothy Ryan

   Article

   Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

   Insert

   PGK1-mRuby (PGK1 Human)
   

   Use

   AAV

   Tags

   mRuby

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLXSN IkB alpha M

   Plasmid

   #12330

   Depositor

   Inder Verma

   Article

   Van Antwerp et al Science. 1996 Nov 1. 274(5288):787-9.

   Insert

   IkB alpha M (Nfkbia Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Dominant-negative mutant. Many signal transductio…

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSNV2 CHER NLS cop1at(335-675)

   Plasmid

   #44972

   Depositor

   Thanos Halazonetis

   Article

   Crefcoeur et al Nat Commun. 2013;4:1779. doi: 10.1038/nc

   Insert

   mCherry-NLS-COP1 (COP1 Mustard Weed)
   

   Use

   Tags

   NLS and mCherry

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-334

   Promoter

   pCMV IE

   Available since

   June 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pB-bsd-Flag-CNP

   Plasmid

   #126608

   Purpose

   Expresses N-terminal Flag-tagged human CNP

   Depositor

   Xiaozhong Wang

   Article

   Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

   Insert

   2'3' cyclic nucleotide phosphodiesterase (CNP Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX-4T-1 3xFlag-ERK2

   Plasmid

   #47573

   Purpose

   Bacterial expression plasmid for GST-3xFlag-tagged ERK2

   Depositor

   Kevin Janes

   Article

   Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

   Insert

   ERK2 (Mapk1 Rat)
   

   Use

   Tags

   3xFlag and GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

6. plenti hSyn PGK1-HALO

   Plasmid

   #220910

   Purpose

   Neuronal specific expression of the fusion construct PGK1-HALO

   Depositor

   Timothy Ryan

   Article

   Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

   Insert

   PGK1-HALO (PGK1 Human)
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRSETa-mEos3.2-A69T

   Plasmid

   #98571

   Purpose

   photoconvertible fluorescent protein mEos3.2-A69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

   Depositor

   Ulrike Endesfelder

   Article

   Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

   Insert

   mEos3.2-A69T
   

   Use

   Tags

   7xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX-4T-1 3xHA-MKK6-EE

   Plasmid

   #47579

   Purpose

   Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK6

   Depositor

   Kevin Janes

   Article

   Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

   Insert

   MKK6-EE (MAP2K6 Human)
   

   Use

   Tags

   3xHA and GST

   Expression

   Bacterial

   Mutation

   Ser207 to Glu, Thr211 to Glu

   Promoter

   Available since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pclbw-PAGFPomp25

   Plasmid

   #58427

   Purpose

   Mammalian expression of photoactivatable GFP targeted to the mitochondrial outer membrane

   Depositor

   David Chan

   Article

   Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

   Insert

   paGFP-omp25 (Synj2bp Mouse)
   

   Use

   Retroviral

   Tags

   PAGFP

   Expression

   Mammalian

   Mutation

   contains aa's 109-145 of mouse omp25

   Promoter

   cmv-bactin

   Available since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pET-30a(+)-Syk-tSH2-PM

    Plasmid

    #111271

    Purpose

    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation, in E.coli strain Rosetta 2 (DE3)

    Depositor

    Carol Post

    Article

    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

    Insert

    murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation (Syk Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y130E mutation to mimic phosphorylation

    Promoter

    T7 promoter

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAdEasy-mRFP-gsk3 beta S9A

    Plasmid

    #24521

    Depositor

    Torsten Wittmann

    Article

    Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

    Insert

    GSK3beta(S9A) (GSK3B Human)
    

    Use

    Adenoviral

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    S9A. Mutant version of GSK3 that lacks the inhibi…

    Promoter

    Available since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pGEM-PH-citrine

    Plasmid

    #131406

    Purpose

    Purpose: synthesis of mRNA encoding a membrane marker. Insert: PH domain of the human phospholipase C delta 1 (PLCD1) fused with the yellow fluorescent protein mCitrine

    Depositor

    Lars Hufnagel

    Article

    Guignard et al Science. 2020 Jul 10;369(6500). pii: 369

    Insert

    PH
    

    Use

    In vitro transcription

    Tags

    citrine yellow fluorescent protein

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3 PARL-FLAG-CT S65D+T69D+S70D

    Plasmid

    #13617

    Depositor

    Luca Pellegrini

    Article

    Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

    Insert

    Presenilin-associated rhomboid-like (PARL, PARL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 65, Thr 69 and Ser70 to Asp

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pRSETa-mEos4b-V69T

    Plasmid

    #98572

    Purpose

    photoconvertible fluorescent protein mEos4b-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression

    Depositor

    Ulrike Endesfelder

    Article

    Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

    Insert

    mEos4b-V69T
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3 PARL-FLAG-CT S65A+T69A+S70A

    Plasmid

    #13616

    Depositor

    Luca Pellegrini

    Article

    Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

    Insert

    Presenilin-associated rhomboid-like (PARL, PARL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Ser 65, Thr 69 and Ser 70 to Alanine

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pRSETa-mKikGR-V69T

    Plasmid

    #98574

    Purpose

    photoconvertible fluorescent protein mKikGR-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

    Depositor

    Ulrike Endesfelder

    Article

    Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

    Insert

    mKikGR-V69T
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHJ425

    Plasmid

    #20683

    Depositor

    Stephen Shaw

    Article

    Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

    Insert

    Ezrin (EZR Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Lys 253, 254, 262, 263 to Asn; and change…

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pETM6_Ptrc_BsMdh_BmHps_BmPhi_Pthl_CaThl_CaCtfAB_CaAdc

    Plasmid

    #129105

    Purpose

    Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus as well as Clostridium acetobutylicum genes required for Acetate-dependent Acetone production

    Depositor

    Eleftherios Terry Papoutsakis

    Article

    Bennett et al Metab Eng. 2018 Jan;45:75-85. doi: 10.10

    Insert

    BsMDH BmHPS BmPHI CaTHL CaCtfAB CaADC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pENTR2b-PTPN12(D199A,C231S)

    Plasmid

    #215516

    Purpose

    Gateway entry vector with a phosphatase-inactive PTP-PEST mutant (D199A,C231S)

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    PTPN12 (PTPN12 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    D199A, C231S (Phosphatase inactive)

    Promoter

    none

    Available since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. PDE4B-Sensor

    Plasmid

    #208929

    Purpose

    in vitro JNK-Sensor for luminescent detection of JNK-activity

    Depositor

    Attila Reményi

    Article

    Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

    Insert

    PDE4B docking sequence+Phospho site and Smbit (PDE4B Human)
    

    Use

    Tags

    HISx6, MBP, and Smbit

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge