19,660 results

1. pET30a-AlkB

   Plasmid

   #79050

   Purpose

   Express wild type AlkB protein in E. coli BL21(DE3)

   Depositor

   Tao Pan

   Article

   Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

   Insert

   AlkB (alkB E.coli)
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   A truncated AlkB with deletion of the amino (N)-t…

   Promoter

   T7

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 myc-RNF114

   Plasmid

   #58295

   Purpose

   mammalian expression of myc tagged RNF114

   Depositor

   Francesca Capon

   Article

   Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

   Insert

   RNF114 (RNF114 Human)
   

   Tags

   his and myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pLSSmOrange-mKate2 caspase-3 biosensor

   Plasmid

   #37132

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

   Insert

   LSSmOrange-DEVD-mKate2
   

   Expression

   Mammalian

   Mutation

   A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

   Promoter

   CMV

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpdCas9 VPR

   Plasmid

   #188509

   Purpose

   Expresses FLAG tagged SpdCas9 VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A/H840A

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP rbFOX2

   Plasmid

   #63086

   Purpose

   Mammalian expression of EGFP tagged FOX2

   Depositor

   Nicolas Charlet-Berguerand

   Insert

   RBFOX2 (RBFOX2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET His6 Sumo TEV LIC cloning vector (2S-T)

   Plasmid

   #29711

   Depositor

   Scott Gradia

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   6xHis, SUMO, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSAM_Cam

   Plasmid

   #91570

   Purpose

   Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Lac promoter expresses transposase. Chloramphenicol resistance on backbone.

   Depositor

   Harry Mobley

   Article

   Bachman et al MBio. 2015 Jun 9;6(3):e00775. doi: 10.11

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

8. 12.4kbVillin-ΔATG

   Plasmid

   #19358

   Depositor

   Deborah Gumucio

   Article

   Madison et al J Biol Chem. 2002 Sep 6. 277(36):33275-8

   Insert

   12.4kb Villin promoter
   

   Expression

   Bacterial

   Mutation

   No ATG - transgenes cloned downstream of the vill…

   Available Since

   Jan. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ9757: CMV-BPNLS_TadA-linkerA-TadA*(8e)-Linker B-SV40 NLS-dCasMINI-V4-linkerC-c-Myc_NLS-3x FLAG-polyA

   Plasmid

   #176270

   Purpose

   Expression of TadA-TadA* (8e)-dCasMINI-V4 in mammalian cells

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   TadA-TadA*(8e)-dCasMINI-V4
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Green Glifon50

    Plasmid

    #126206

    Purpose

    green fluorescent glucose sensor for mammalian cells

    Depositor

    Tetsuya Kitaguchi

    Article

    Mita et al Anal Chem. 2019 Apr 2;91(7):4821-4830. d

    Insert

    Green Glifon50
    

    Expression

    Mammalian

    Available Since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKM446 (Flag-DAS+4))

    Plasmid

    #108321

    Purpose

    Orbit integrating plasmid for C-terminal tagging with degradation tag DAS+4, using hygromycin as a selection marker.

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Insert

    attB-Flag-DAS+4
    

    Use

    Orbit integrating plasmid

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCI-Neo HA-hSnoN

    Plasmid

    #10908

    Depositor

    Bob Weinberg

    Article

    Sun et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

    Insert

    SnoN (SKIL Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pCMV3flag8SHARPIN

    Plasmid

    #50014

    Purpose

    Mammalian expression of human SHARPIN with a c-terminal 3xFLAG tag, and hygromycin selection.

    Depositor

    Martin Dorf

    Article

    Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

    Insert

    SHANK-associated RH domain interactor (SHARPIN Human)
    

    Tags

    3 x FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBabe EGFR(L858R/T790M)

    Plasmid

    #32073

    Depositor

    Matthew Meyerson

    Article

    Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    L858R/T790M

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMyNT

    Plasmid

    #42191

    Depositor

    Annabel Parret, Matthias Wilmanns

    Article

    Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

    Type

    Empty backbone

    Tags

    His6

    Expression

    Bacterial

    Promoter

    ColE1

    Available Since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. Flag-HA-USP15

    Plasmid

    #22570

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP15 (USP15 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCF806-shRen.713

    Plasmid

    #186712

    Purpose

    Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Non-targeting control shRNA (shRen.713).

    Depositor

    Christof Fellmann

    Article

    Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

    Insert

    shRen.713
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3 HA Arf6 DN T27N

    Plasmid

    #10831

    Depositor

    Thomas Roberts

    Article

    Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

    Insert

    Arf6 T27N (ARF6 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T27N. Defective in GTP binding. Dominant negati…

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSCV-N EBNA3C

    Plasmid

    #37958

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    EBNA3C (EBNA-3B/EBNA-3C H. Herpesvirus 4 (EBV))
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    PKG

    Available Since

    Aug. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTA7001-DEST

    Plasmid

    #71745

    Purpose

    GATEWAY compatible derivative of plant binary vector pTA7001 from Nam Chua's laboratory at The Rockefeller University, Dexamethasone inducible

    Depositor

    Roger Innes

    Article

    Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    GAL4

    Available Since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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