159,530 results

1. pHAGE mCherry-Rab11A

   Plasmid

   #228035

   Purpose

   Mammalian lentiviral expression of mCherry-labeled Rab11.

   Depositor

   Wade Harper

   Insert

   Rab11A (RAB11A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF0208

   Plasmid

   #142556

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FOXP2 (FOXP2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW57.1 tTA SDHB

   Plasmid

   #177977

   Purpose

   All-in-one dox-repressible expression of cDNA

   Depositor

   David Sabatini, Jessica Spinelli

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   SDHB (SDHB Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Silent point mutations to SDHB sgRNA target

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFPC1-hVAP-A

   Plasmid

   #104447

   Purpose

   Expression of human VAP-A fused to GFP in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

   Insert

   vesicle-associated membrane protein-associated protein A (VAPA Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-CMV-Flag-CDK12_IDG-K

   Plasmid

   #135281

   Purpose

   Gateway destination clone of CDK12 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics

   Depositor

   Ben Major

   Insert

   Flag-CDK12 (CDK12 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRPN265NB-enh-MNase

   Plasmid

   #166035

   Purpose

   Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

   Depositor

   Marc Timmers

   Article

   Nizamuddin et al Nucleic Acids Res. 2021 May 21;49(9):e49

   Insert

   Glutathione-S-Transferase/enhancer/MNase
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   lac promoter / tac promoter

   Available since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-Frb-EGFP-HOTag6

   Plasmid

   #106919

   Purpose

   Express Frb-EGFP-HOTag6 fusion in mammalian cells.

   Depositor

   Xiaokun Shu

   Article

   Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

   Insert

   Frb-EGFP-HOTag6
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. phage UbiC NLS HA stdMCP stdHalo

   Plasmid

   #104999

   Purpose

   Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag for mRNA labeling

   Depositor

   Jeffrey Chao

   Article

   Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

   Insert

   stdMCP-stdHalo
   

   Use

   Lentiviral

   Tags

   HA tag, NLS, Two synonymous copies of the Halo-ta…

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   UbiC

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBTK1009 (pSL9)

   Plasmid

   #191004

   Purpose

   Expresses E2 Crimson on a broad host range pBBR1 origin plasmid

   Depositor

   Jeffrey Barrick

   Article

   Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

   Insert

   E2-Crimson
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   CP25

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. gag-MCP-pol

    Plasmid

    #211370

    Purpose

    Packaging plasmid for v3 PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    MMLVgag-MCP-pol
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pm-iATPSnFR1.0

    Plasmid

    #102548

    Purpose

    membrane displayed ATP sensor for mammalian cells

    Depositor

    Baljit Khakh

    Article

    Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

    Insert

    iATPSnFR1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21a-MetRS-His

    Plasmid

    #124115

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Methionine--tRNA ligase (metG Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOxyR_gfp

    Plasmid

    #194154

    Purpose

    Green Fluorescent Reporter of intracellular hydrogen peroxide

    Depositor

    Sven Panke

    Article

    Kardashliev et al Chem Sci. 2021 Oct 29;12(44):14766-14772

    Insert

    transcriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PoxyR, PoxyS

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHis17_ParM _1

    Plasmid

    #78201

    Purpose

    Expression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor

    Depositor

    Martin Webb

    Article

    Kunzelmann et al J Biol Chem. 2009 Nov 27;284(48):33130-8

    Insert

    parM (K33A I27C T174A T175N C287A)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    K33A I27C T174A T175N C287A

    Promoter

    unknown

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSL0527 (pDonor)

    Plasmid

    #130634

    Purpose

    Encodes a mini-transposon derived from V. cholerae CAST system, with CmR cargo gene. Total transposon size = 977 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchCAST donor DNA (Mini-Tn)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTripz NEO DEST

    Plasmid

    #209072

    Purpose

    Modified DOX-ON inducible destination vector with a c-terminal 3X flag tag to be used for LR recombination reactions to inducibly express cDNAs of interest

    Depositor

    Matthew Oser

    Article

    Oser et al Cancer Discov. 2019 Feb;9(2):230-247. do

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector to be used…

    Tags

    3X FLAG

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA HA FAK K454R

    Plasmid

    #50501

    Depositor

    Kenneth Yamada

    Article

    Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

    Insert

    protein tyrosine kinase, mutated
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K454R

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pDZ50

    Plasmid

    #41948

    Depositor

    Stephen Elledge

    Article

    Zhang et al Cell. 2006 Aug 11;126(3):529-42.

    Insert

    ubiquitin specific peptidase 28 (USP28 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 20, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pME tdTomato-CAAX

    Plasmid

    #135204

    Purpose

    Red fluorescent tag

    Depositor

    David Tobin

    Article

    Oehlers et al Nature. 2015 Jan 29;517(7536):612-5. doi

    Insert

    tdTomato
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-PGK-KRAS4B(G12V)

    Plasmid

    #35633

    Depositor

    Daniel Haber

    Article

    Singh et al Cell. 2012 Feb 17;148(4):639-50.

    Insert

    K-Ras4B(G12V) (KRAS Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Glycine 12 to Valine

    Promoter

    PGK

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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