We narrowed to 65,936 results for: des.1

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pFX-mCherry-EEA1

Plasmid

#174452

Purpose

Express mCherry with early endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with early endosome-targeting sequence

Tags

early endosomal antigen 1 (EEA1)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pEF-XMAS-1xStop

Plasmid

#164411

Purpose

Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.

Depositor

Xiao Wang

Article

Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

Insert

mCherry

Use

CRISPR and Synthetic Biology

Tags

T2A-GFP

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-neo-PP1-Alpha

Plasmid

#171267

Purpose

Mammalian expression of 3x-Flag-tagged human PP1 alpha catalytic subunit

Depositor

Steven Balk

Article

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

3x-Flag-tagged human PP1 alpha catalytic subunit (PPP1CA Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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GfaABC1D BARK D110A p2A mCherry

Plasmid

#117692

Purpose

GfaABC1D-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for astrocyte Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs D110A peptide

Use

AAV

Tags

HA / FLAG

Mutation

D110A

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pXG290

Plasmid

#141284

Purpose

OE of HRI

Depositor

Martin Kampmann

Article

Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

Insert

HRI (EIF2AK1 Human)

Tags

mClover

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-iFlpV

Plasmid

#140137

Purpose

can be used to generate AAV virus that will express light-inducible site-specific iFlpV recombinase

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Has Service

AAV PHP.eB and AAV1

Insert

iFlpV

Use

AAV

Promoter

EF1a

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-ERGIC53-IRES-Puromycin

Plasmid

#134859

Purpose

Expresses EGFP-tagged ERGIC marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

ERGIC53 (Lman1 Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

Plasmid

#134661

Purpose

Expresses EGFP-tagged synaptic vesicle protein

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

vesicle-associated membrane protein 2 (VAMP2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pMito-mCherry-FRB_IRES_FKBP(I)-GBPen

Plasmid

#128267

Purpose

Bicistronic vector to express mitochondrial targeted mCherry-FRB (MitoTrap) as well as 1x FKBP-GBPenhancer (GFP Nanobody with 1 FKBP domain).

Depositor

Stephen Royle

Article

Kuey et al J Cell Sci. 2019 Nov 6;132(21). pii: jcs

Inserts

MitoTrap
FKBP(I)-GBPen

Expression

Mammalian

Promoter

CMV and IRES

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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OmpA-mNG-sfTq2ox

Plasmid

#124220

Purpose

Periplasmic OM bound mNeongreen superfolder mTurquoise2 ox tandem, ~40 % energy transfer. pNM092 - pTHV037-Ompa-SA1-177-(SA-1)-LEDPPAEL-mNG-sfTq2ox,

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

Insert

OmpA-mNG-sfTq2ox

Expression

Bacterial

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-shRab27a

Plasmid

#120930

Purpose

conditionally knockdown Rab27a in mouse cell lines

Depositor

Mien-Chie Hung

Article

Yang et al Cell Res. 2018 Aug;28(8):862-864. doi: 1

Insert

shRab27a (Rab27a Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSH231-EF1-BLST-dCas9-VPR

Plasmid

#115148

Purpose

Safe harbor site 231 knock-in vector with BlastR-dCas9-VPR expression cassette

Depositor

Raymond Monnat, Michael Phelps

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

BlastR-P2A-dCas9-VPR

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-cMYC promoter

Plasmid

#114724

Purpose

reporter luciferase construct

Depositor

Martine Roussel

Article

Roussel et al Oncogene. 1994 Feb;9(2):405-15.

Insert

C-Myc promoter region

Tags

luciferase

Expression

Mammalian

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pSH231-EF1-GFP-HYGRO

Plasmid

#115144

Purpose

Safe harbor site 231 knock-in vector with GFP-HygromycinR expression cassette

Depositor

Raymond Monnat, Michael Phelps

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

GFP

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry-EXO1a

Plasmid

#111629

Purpose

Expresses human WT Exonuclease 1a (EXO1a) tagged with mCherry in mammalian cells

Depositor

Marco Muzi-Falconi

Article

Sertic et al Proc Natl Acad Sci U S A. 2011 Aug 16;10

Insert

Exonuclease 1a (EXO1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1neo-NLucYAP15

Plasmid

#107610

Purpose

measure LATS activity by Luciferase Assay

Depositor

Xiaolong Yang

Article

Azad et al Nat Commun. 2018 Mar 13;9(1):1061. doi:

Insert

NLucYAP15

Expression

Mammalian

Available Since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-tdTomatof

Plasmid

#104060

Purpose

Also known as hSyn1-tdTomato-f. AAV expression of Farnylsated tdtomato for tracing

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

tdTomato

Use

AAV

Promoter

hSyn

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_NES-his-CaMPARI2-F391W-G395D-WPRE-SV40

Plasmid

#101063

Purpose

AAV vector expressing CaMPARI2_F391W-G395D (Kd = 530nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Insert

CaMPARI2_F391W-G395D

Use

AAV

Tags

FLAG-HA-myc and NES_his

Promoter

hsyn (synapsin-1)

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-PANX1(HCVp)

Plasmid

#87698

Purpose

Express HCV protease-activatible pannexin-1 in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PANX(HCVp)

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pJH373

Plasmid

#86840

Purpose

mamalian expression of anti-CRISPR protein AcrIIA2

Depositor

Joseph Bondy-Denomy

Article

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA2

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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