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We narrowed to 9,102 results for: sgRNA

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Showing: 7061 - 7080 of 9102 results

  1. pEN313 - Rad21-Halo-Frt-PGK-EM7-NeoR-bpA-Frt targeting

    Plasmid
    #156431
    Purpose
    Targeting vector to introduce an Halotag cassette at the mouse Rad21 locus using NEOMYCINE selection. Designed for using with sgRNA CCACGGTTCCATATTATCTG
    Depositor
    Elphege Nora
    Publication
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAD21, Halotag (Rad21 Mouse)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    none
    Available since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    156431-plasmid-map-sequence-id-310238

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  2. pU6-(BsaI)_CBh-Cas9-T2A-mCherry

    Plasmid
    #135012
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Publication
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    135012-plasmid-map-sequence-id-267596

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  3. pBHM2681

    Plasmid
    #241726
    Purpose
    Construction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains ptxD marker for selection on media containing phosphite as a sole phosphorus source
    Depositor
    Hiten Madhani
    Publication
    Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Sept. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    241726-plasmid-map-sequence-id-479856

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  4. pYPQAT07

    Plasmid
    #223379
    Purpose
    T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    AtUBQ10-SpRY-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223379-plasmid-map-sequence-id-452337

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  5. qTAG-C-mStayGold-Puro-H2BC11

    Plasmid
    #227332
    Purpose
    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H2BC11 Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227332-plasmid-map-sequence-id-452132

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  6. qTAG-C-mStayGold-Puro-PXN

    Plasmid
    #227317
    Purpose
    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PXN Homology Arms flanking a mStayGold-2A-Puro Cassette (PXN Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227317-plasmid-map-sequence-id-452087

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  7. qTAG-C-mStayGold-Puro-CANX

    Plasmid
    #227281
    Purpose
    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the CANX locus. For cilia visualization. To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279)
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CANX Homology Arms flanking a mStayGold-2A-Puro Cassette (CANX Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227281-plasmid-map-sequence-id-452050

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  8. qTAG-C-miRFP670nano3-Blast-H3C2

    Plasmid
    #207784
    Purpose
    Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H3C2 Homology Arms flanking a miRFP670nano3-Blast Cassette (H3C2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207784-plasmid-map-sequence-id-416184

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  14. pX330-HA-dSpCas9

    Plasmid
    #92112
    Purpose
    Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead/inactive SpCas9
    Use
    CRISPR
    Tags
    HA tag and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    Cbh
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    92112-plasmid-map-sequence-id-197503

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  16. pLentiCMVblast_SLC38A2_wt

    Plasmid
    #156180
    Purpose
    CMV-driven expression of sgRNA-resistant SLC38A2 wild-type cDNA.
    Depositor
    Alec Kimmelman
    Publication
    Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SLC38A2 (SLC38A2 Human)
    Expression
    Mammalian
    Mutation
    Silent mutations to prevent targeting by sgRNA se…
    Promoter
    CMV
    Available since
    Sept. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    156180-plasmid-map-sequence-id-311605

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  19. qTAG-N-Solo-mStayGold-TJP1

    Plasmid
    #227300
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the TJP1 locus. For tight junction visualization. To be co-transfected with sgRNA plasmid px330-TJP1 (Addgene #227299)
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TJP1 Homology Arms flanking a mStayGold Tag (TJP1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227300-plasmid-map-sequence-id-451538

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  20. qTAG-C-Solo-mStayGold-CANX

    Plasmid
    #227280
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the CANX locus. For ER visualization. To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279)
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CANX Homology Arms flanking a mStayGold Tag (CANX Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227280-plasmid-map-sequence-id-452047

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Showing: 7061 - 7080 of 9102 results