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Cavin4-mCherry

Plasmid

#176007

Purpose

Mammalian expression of Mouse Cavin4-mCherry. Parton lab clone JSG

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Cavin4 (Cavin4 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-Prom1-TEVp-TwinStrep-His / IRES2 / Pcdh21-TEVp-Myc-Flag

Plasmid

#210820

Purpose

Mammalian overexpression vector for polycistronic co-expression of C-terminally Strep and His tagged human Prom1s1 (WT) and C-terminally Myc and Flag tagged human Pchd21 (WT)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Inserts

Prominin 1 (PROM1 Human)
Protocadherin 21 (CDHR1 Human)

Tags

Myc, 3xFlag and TwinStrep, 10xHis

Expression

Mammalian

Promoter

CMV and CMV / IRES2

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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HCN4 scFv [N114/10]

Plasmid

#190526

Purpose

Mammalian Expression of HCN4 scFV. Derived from hybridoma N114/10.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

HCN4 (Homo sapiens) recombinant scFV (HCN4 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Beta4-spectrin [N393/76R]

Plasmid

#177542

Purpose

Mammalian Expression Plasmid of anti-Beta4-spectrin (Human). Derived from hybridoma N393/76.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Beta4-spectrin (Homo sapiens) recombinant mouse monoclonal antibody (SPTBN4 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-VAPA/B [N479/107R]

Plasmid

#188163

Purpose

Mammalian Expression Plasmid of anti-VAPA and -VAPB (Rat). Derived from hybridoma N479/107.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-VAPA and -VAPB (Rattus norvegicus) recombinant mouse monoclonal antibody (Vapa, Vapb Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Nav1.7 Na+ channel [N68/6R]

Plasmid

#177566

Purpose

Mammalian Expression Plasmid of anti-Nav1.7 Na+ channel (Human). Derived from hybridoma N68/6.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Nav1.7 Na+ channel (Homo sapiens) recombinant mouse monoclonal antibody (SCN9A Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-TrpV1 [N221/17R]

Plasmid

#177517

Purpose

Mammalian Expression Plasmid of anti-TrpV1 (Rat). Derived from hybridoma N221/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpV1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpv1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pQC hGAB.wt V5-His IRES G418

Plasmid

#110333

Purpose

Mammalian retroviral expression of glutaminase 2 isoform GAB (wild type) with V5 and 6xHis tags

Depositor

Sandra Martha Gomes Dias

Article

Dias et al Oncogene. 2020 Jan;39(3):690-702. doi: 1

Insert

GLS2 Glutaminase 2 (GLS2 Human)

Use

Retroviral

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-QKI-7 [N183/15R]

Plasmid

#177507

Purpose

Mammalian Expression Plasmid of anti-QKI-7 (Human). Derived from hybridoma N183/15.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-QKI-7 (Homo sapiens) recombinant mouse monoclonal antibody (QKI Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-IDR1-mCherry-Cry2

Plasmid

#177125

Purpose

Express IDR1 fragment of YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay

Depositor

Wenbo Li

Article

Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

Insert

YTHDC1 (IDR1 domain) (YTHDC1 Human)

Tags

mCherry-CRY2

Expression

Mammalian

Mutation

IDR1 domain only

Promoter

SFFV

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Npas4 [N408/79]

Plasmid

#177547

Purpose

Mammalian Expression Plasmid of anti-Npas4 (Rat). Derived from hybridoma N408/79.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Npas4 (Rattus norvegicus) recombinant mouse monoclonal antibody (Npas4 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-nNOS/NOS1 [L121/133R]

Plasmid

#188170

Purpose

Mammalian Expression Plasmid of anti-nNOS/NOS1 (Human). Derived from hybridoma L121/133

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-nNOS/NOS1 (Homo sapiens) recombinant mouse monoclonal antibody (NOS1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Contactin/F3 [K73/20R]

Plasmid

#177447

Purpose

Mammalian Expression Plasmid of anti-Contactin/F3 (Rat). Derived from hybridoma K73/20.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Contactin/F3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Cntn1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pαH-S-GSAS-P1-like

Plasmid

#173789

Purpose

Mammalian expression of SARS-CoV-2 Spike protein P-1-like variant (K417N)

Depositor

Priyamvada Acharya

Article

Gobeil et al Science. 2021 Jun 24. pii: science.abi62

Insert

Spike S-GSAS- P-1-like variant (K417N) (S Severe acute respiratory syndrome coronavirus 2)

Tags

HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-EAAC1 [N420/24R]

Plasmid

#206572

Purpose

Mammalian Expression Plasmid of anti-EAAC1 (Rat). Derived from hybridoma N420/24.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-EAAC1 (Rattus norvegicus) recombinant Mouse monoclonal antibody (Slc1a1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-EAAC1 [N180/41R]

Plasmid

#206615

Purpose

Mammalian Expression Plasmid of anti-EAAC1 (Rat). Derived from hybridoma N180/41.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-EAAC1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Slc1a1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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JDW 763 (pCS2-EYFP-hsKRAS4B-WT)

Plasmid

#156408

Purpose

pCS2 CMV expression vector driving EYFP fused human KRAS4B

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human wild type KRAS4B-WT (KRAS Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-NTID194-GSQ-PMLiva3MAS-P2A-BLAST

Plasmid

#208039

Purpose

Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; 3MAS mutant; K>R sumoylation sites, mutated SIM); selection with blasticidin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

Insert

PML (isoform IVa; 3MAS) (PML Human)

Use

Lentiviral

Tags

FLAG, N-terminal Split-TurboID

Mutation

3MAS mutant: K65R, K160R and K490R; mutated SIM

Promoter

EFS

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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PSD-95 scFv [K28/42]

Plasmid

#190488

Purpose

Mammalian Expression of PSD-95 scFV. Derived from hybridoma K28/42.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

PSD-95 (Homo sapiens) recombinant scFV (DLG4 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-TrpV1 [N221/12R]

Plasmid

#188224

Purpose

Mammalian Expression Plasmid of anti-TrpV1 (Rat). Derived from hybridoma N221/12

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpV1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpv1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-NrCAM [N343/26R]

Plasmid

#177534

Purpose

Mammalian Expression Plasmid of anti-NrCAM (Mouse). Derived from hybridoma N343/26.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-NrCAM (Mus musculus) recombinant mouse monoclonal antibody (Nrcam Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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GluN2B/NR2B glutamate receptor scFv [N59/36]

Plasmid

#190563

Purpose

Mammalian Expression of GluN2B/NR2B glutamate receptor scFV. Derived from hybridoma N59/36.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GluN2B/NR2B glutamate receptor (Rattus norvegicus) recombinant scFV (Grin2b Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Pvalb-2A-Dre Flp-in replacement vector

Plasmid

#61571

Purpose

Recombinase-mediated cassette exchange in ES cells to insert the Dre recombinase gene at the mouse Pvalb gene stop codon

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

Pvalb-2A-Dre (Pvalb Mouse, D6 Bacteriophage)

Use

Recombinase-mediated cassette exchange using flp …

Available Since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Omicron.BA.5-RBD

Plasmid

#213478

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.BA.5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/Omicron.BA.5 RBD (S )

Tags

6 His Tag

Expression

Mammalian

Mutation

G339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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PSD-93/Chapsyn-110 scFv [N18/28]

Plasmid

#190535

Purpose

Mammalian Expression of PSD-93/Chapsyn-110 scFV. Derived from hybridoma N18/28.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

PSD-93/Chapsyn-110 (Rattus norvegicus) recombinant scFV (Dlg2 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS/EG5-RBD

Plasmid

#213410

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.EG5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/EG5-RBD (S )

Tags

6 His Tag

Expression

Mammalian

Mutation

G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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c-Fos scFv [N486/76]

Plasmid

#190560

Purpose

Mammalian Expression of c-Fos scFV. Derived from hybridoma N486/76.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

c-Fos (Homo sapiens) recombinant scFV (FOS Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pPICZc-Ub-R-HsACTB*-thymosinB-8His

Plasmid

#111149

Purpose

Expresses arginylated human beta-actin fused with thymosin beta and a His tag.

Depositor

Mohan Balasubramanian

Article

Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

Insert

ACTB (ACTB Human)

Use

Pichia pastoris integration plasmid

Tags

Thymosin beta and a His-tag

Expression

Yeast

Mutation

N-terminal arginylation

Promoter

AOX1

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-Calretinin [L122/6R]

Plasmid

#177468

Purpose

Mammalian Expression Plasmid of anti-Calretinin (Human). Derived from hybridoma L122/6.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Calretinin (Homo sapiens) recombinant mouse monoclonal antibody (CALB2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-ms-c-Myc-L420P

Plasmid

#50776

Purpose

retroviral expression of mouse c-Myc L420P mutant

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

c-Myc L420P (MYC, Myc Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

L420P (does not bind to Max)

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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