Addgene

Showing: 7101 - 7120 of 15153 results

Results per page:

Sort results by:

pRS416 ATG5-E141D-HA-ATG12-ATG16

Plasmid

#166851

Purpose

Expresses mutant E141D ATG5, HA-ATG12 ATG16 in yeast.

Depositor

Daniel Klionsky

Article

Kim et al Elife. 2016 Jan 26;5. pii: e12245. doi:

Insert

ATG5 ATG12 ATG16 (ATG5, ATG12, ATG16 Budding Yeast)

Use

Tags

HA-ATG12

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
KRAS-V14I

Plasmid

#154427

Purpose

V14I Kras with Flag tag inserted into N-terminal domain between 6Xhis tag and TEV cleavage site.

Depositor

Kenneth Westover

Article

Bera et al J Biol Chem. 2019 Sep 20;294(38):13964-1

Insert

KRAS (KRAS Human)

Use

Tags

6xHis-tag and TEV protease cleavage sequence

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
t1-400

Plasmid

#166577

Purpose

For expression of human talin head (residues 1-400) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.

Depositor

Vesa Hytönen

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 Dec 22;11

Insert

Tln1 Head (1-400) (TLN1 Human)

Use

Tags

His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

Expression

Bacterial

Mutation

residues 1-400 only

Promoter

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 myc-mβTrCP WD2-7 (292-569)

Plasmid

#62978

Purpose

Expresses myc-tagged mouse βTrCP residues 292-569

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

BTRC (Btrc Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

codes for residues 292-569

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pHAGE2-TetOminiCMV-KS

Plasmid

#136617

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Klf4, Sox2

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Klf4-IRES-Sox2 (Klf4, Sox2 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pPRIME-CMV-GFP-shCrh1

Plasmid

#132709

Purpose

Encodes short hairpin RNA (shRNA) #1 that targets the 3’-untranslated region of the rat Crh gene

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

shCrh(1) (Crh Rat)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ239-RVR

Plasmid

#108863

Purpose

FnCpf1 Gateway entry plasmid with N623R, K629R and N633R mutations

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

Insert

FnCpf1-RVR (FnCpf1 with N623R, K629R and N633R mutations)

Use

CRISPR; Gateway compatible cpf1 entry clone

Tags

NLS

Expression

Plant

Mutation

N623R, K629R and N633R, Cpf1 is rice codon optimi…

Promoter

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Drosha270-1374S300/302A

Plasmid

#62533

Purpose

N-terminal aa1-269 of Drosha is deleted and serines 300 &302 are mutated to alanine

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

truncated human Drosha mutant (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-269 and changed serines 300…

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-mPC1_4077-eGFP-3HA

Plasmid

#108390

Purpose

Express cytoplasmic tail mouse PC1 from amino acid 4077 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

Pkd1 (Pkd1 Mouse)

Use

Tags

eGFP-3HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-mPC1_4119-3HA

Plasmid

#108395

Purpose

Express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

Pkd1 (Pkd1 Mouse)

Use

Tags

3HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
zgc:111821_R (OZ598)

Plasmid

#35238

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting zgc:111821 (zgc:111821 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

March 5, 2012

Availability

Academic Institutions and Nonprofits only
zgc:111821_L (OZ597)

Plasmid

#35237

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting zgc:111821 (zgc:111821 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

March 5, 2012

Availability

Academic Institutions and Nonprofits only
CACNA1F_R (OZ508)

Plasmid

#27183

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting cacna1f (cacna1f Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2011

Availability

Academic Institutions and Nonprofits only
Encephalopsin (Opn3)_L (OZ541)

Plasmid

#28066

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting Encephalopsin (Opn3) (opn3 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only
Encephalopsin (Opn3)_R (OZ542)

Plasmid

#28067

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting Encephalopsin (Opn3) (opn3 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only
xylt1_L (OZ509)

Plasmid

#27184

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting xylt1 (xylt1 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
pEMS1707

Plasmid

#29295

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple235 (TRH Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Expression

Mutation

Promoter

Available since

Nov. 4, 2011

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
psen2_R (OZ578)

Plasmid

#35218

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting psen2 (psen2 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
psen2_L (OZ577)

Plasmid

#35217

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting psen2 (psen2 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
unc119b_R (OZ582)

Plasmid

#35222

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting unc119b (unc119b Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

March 16, 2012

Availability

Academic Institutions and Nonprofits only