We narrowed to 16,337 results for: LEA
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Plasmid#180760PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Asp115Ala (residues 1-327)DepositorInsertα-COPI-WD40-Asp115Ala
TagsC-terminal strep tagExpressionMammalianMutationEncodes residues 1-327; mutations: Asp115Ala, Leu…PromoterCMVAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
PET-B-HeFSpCas9-NLS-6xHis
Plasmid#207392PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-HeFSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationN497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A…PromoterT7Available SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCWXPG-UBI-Ngn3PDX1MafA
Plasmid#114303PurposeConstitutive expression of Ngn3, PDX1 and MafA proteins from a precursor with self-cleavable 2A peptidesDepositorInsertNgn3 (NEUROG3 Human)
UseLentiviralMutationNgn3, PDX1 and MafA proteins are expressed from a…Available SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEN017-SpCas9-hiNLS-t-M4
Plasmid#234455PurposeExpresses hiNLS variant t-M4 inside 0C triNLS-SpCas9-NGGDepositorInsertSpCas9 hiNLS t-M4
UseCRISPRTagsHis6, Nucleoplasmin, SV40, and c-MycExpressionBacterialMutationCys at positions 80 and 574 have been mutated to …Available SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-HTR6-SNAPf-TRIP
Plasmid#189617PurposeExpresses cilia-targeted RhoA inhibitory peptide, tet-inducibleDepositorInsertHTR6-TRIP_alpha
UseAAVTagsalphaTag in the N-term of HTR6, SNAP-tag in the C…PromoterTREAvailable SinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-dL5**-mCER-TRF1
Plasmid#168176PurposeExpresses FAP (dL5**) fused to mCerulean and the telomere binding protein TRF1DepositorAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.C.NWS_mCherry-NLS
Plasmid#178282PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.C.NWS and Nuclear Localization SignalPromotereF1aAvailable SinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-ArgiNLS-EGFP
Plasmid#220601PurposeCre-dependent expression of a single-cell discriminating version of EGFP fluorescent proteinDepositorInsertEGFP
UseAAV and Cre/LoxTagsArgiNLSExpressionMammalianPromoterCAGAvailable SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAG-mGreenLantern-KASH
Plasmid#191094PurposeTo express a bright monomeric green FP to label the eucaryotic nuclear envelope. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsertmGreenLantern-KASH
UseAAV and Synthetic BiologyTagsmGreenLantern fused to the KASH domain of Nesprin…ExpressionMammalianMutationOptimizzation was done using the Human codon usagePromoterCMV enhancer and CAGAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pELPS-eDHFR-YFP-T2A-Renilla
Plasmid#193253PurposeTriple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferaseDepositorInsertThe eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
UseLentiviralTagsRenilla luciferase (rLuc) and yellow fluorescent …MutationNonePromoterEF1aAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
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Ad-Flag-XBP1s
Plasmid#63678PurposeAdenoviral vector for XBP1s expressionDepositorAvailable SinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKH3-TRIM28-C65A/C68A
Plasmid#92199PurposeMammalian overexpression vector for TRIM28; catalytically inactive (mutated on C65A and C68A)DepositorInsertTif1b (TRIM28 Synthetic, Human)
TagsHAExpressionMammalianMutationChanged Cysteines 65 and 68 to Alanines.Available SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
NLS-PyronicSF/pCMV-myc-nuc
Plasmid#124814PurposeHighly resposive GFP-based pyruvate nanosensor with a nuclear localization signal.DepositorInsertPyronicSF
TagsNuclear Localization SignalExpressionMammalianPromoterCMVAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EphrinB3-Flag
Plasmid#155012PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 from pcDNA3.1DepositorInsertEphrinB3 (EPHB3 Human)
TagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianPromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-actin-V159N
Plasmid#118383PurposeGateway compatible donor vector with actin-V159N.DepositorAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.C.VSVg_mCherry-NLS
Plasmid#178214PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.C.VSVg and Nuclear Localization SignalPromotereF1aAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.C.V5_mCherry-NLS
Plasmid#178215PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.C.V5 and Nuclear Localization SignalPromotereF1aAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 1154)
Plasmid#229840PurposeA Piggybac compatible, tet-on, dual reporter encoding luciferase followed by a P2A cleavage peptide and then an H2A fused mCherry for nuclear labeling.DepositorInsertLuciferase
ExpressionMammalianAvailable SinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-vCreDIO hChR2(H134R)-EYFP
Plasmid#55643PurposevCre-Dependent ChR2-EYFPDepositorInserthChR2(H134R)-EYFP
UseAAV; Vcre-dependent chr2-eyfpTagsEYFPExpressionMammalianPromoterEf1aAvailable SinceAug. 8, 2014AvailabilityAcademic Institutions and Nonprofits only