We narrowed to 8,677 results for: sgrna

1. pGL3-U6-pegRNA_SRD5A3-EGFP

   Plasmid

   #159781

   Purpose

   S. pyogenes pegRNA for C to A mutation at the SRD5A3 site of human cells using prime editing

   Depositor

   Xiaolong Wang

   Article

   Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

   Insert

   spacer of pegRNA targeting SRD5A3 gene (SRD5A3 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. FUG-T2A-Cas9

   Plasmid

   #75346

   Purpose

   Ubiquitin promoter expresses GFP and humanized spCas9 (from PX330) via a T2A motif. For cell transfection or use in lentiviral packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

   Depositor

   Bryan Luikart

   Article

   Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP (via t2a)

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYPQ141-ZmUbi-RZ-Aac

   Plasmid

   #129685

   Purpose

   (Empty backbone) Gateway entry clone with sgRNA under ZmUbi promoter

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   Empty backbone
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CSAC-Bmal1

   Plasmid

   #168296

   Purpose

   Expresses sgRNA in mammalian cells

   Depositor

   Han Kyoung Choe

   Article

   Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

   Insert

   hU6-sgBmal1-1-hU6-sgBmal1-3
   

   Use

   AAV

   Tags

   mCherry

   Promoter

   U6, hSyn

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Lenti-evoA1-seBEN_empty

   Plasmid

   #174701

   Purpose

   Small-molecule controlled base editing

   Depositor

   Rahul Kohli

   Article

   Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

   Insert

   Lenti-evoA1-seBEN
   

   Use

   Lentiviral

   Tags

   myc tag

   Promoter

   EFS/U6

   Available Since

   Nov. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. sgLacZs

   Plasmid

   #171185

   Purpose

   Expresses sgRNA

   Depositor

   Han Kyoung Choe

   Article

   Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

   Insert

   hU6-sgLacZ1-hU6-sgLacZ2
   

   Use

   AAV

   Tags

   mCherry

   Promoter

   U6, hSyn

   Available Since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSLQ2804 pHR: U6-SpsgTRE3G CMV-PYL1-VPR-IRES-mCherry

   Plasmid

   #84258

   Purpose

   Expresses Sp sgTRE3G gRNA with ABA-inducible VPR and mCherry for OR gate

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   Sp sgTRE3G
   PYL1-VPR
   

   Use

   CRISPR and Lentiviral

   Tags

   IRES-mCherry and PYL1

   Expression

   Mammalian

   Promoter

   CMV and mouse U6

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDECKO-mCherry MALAT1_Enhancer.1

   Plasmid

   #78536

   Purpose

   Experimental plasmid

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6 (for expressing sgRNA) and U6 promoter

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBTK614

   Plasmid

   #110608

   Purpose

   BTK assembled plasmid - Encodes dCas9 with no targeting sgRNA (used in Stage 2 assembly to repress target genes)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   dCas9
   

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSLQ2827 pHR: U6-SpsgSV40 CMV-PYL1-KRAB-IRES-mCherry

    Plasmid

    #84260

    Purpose

    Expresses Sp sgSV40 gRNA with ABA-inducible KRAB and mCherry for diametric regulation

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    Sp sgSV40
    PYL1-KRAB
    

    Use

    CRISPR and Lentiviral

    Tags

    IRES-mCherry and PYL1

    Expression

    Mammalian

    Promoter

    CMV and mouse U6

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBTK601

    Plasmid

    #110607

    Purpose

    BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    Cas9
    

    Use

    Synthetic Biology

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMC-Z-SpCas9-gRNA-repeat-polyT-11

    Plasmid

    #197781

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    gRNA repeat for U6 sgRNA assembly
    

    Expression

    Plant

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. gRNA-HEK3-Puro

    Plasmid

    #136282

    Purpose

    Expresses a guide RNA targeting HEK3 site

    Depositor

    Zhaohui Ye

    Article

    McGrath et al Nat Commun. 2019 Nov 25;10(1):5353. doi:

    Insert

    sgRNA-HEK3
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDECKO-mCherry MALAT1_Exon.1

    Plasmid

    #78540

    Purpose

    Experimental plasmid

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and U6 (for expressing sgRNA)

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Sha2Cas9

    Plasmid

    #192140

    Purpose

    Expresses Sha2Cas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

    Insert

    Sha2Cas9
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SpeCas9

    Plasmid

    #192139

    Purpose

    Expresses SpeCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

    Insert

    SpeCas9
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Sa-SchCas9

    Plasmid

    #192137

    Purpose

    Expresses Sa-SchCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wang et al Adv Sci (Weinh). 2022 Feb;9(4):e2104789.

    Insert

    Sa-SchCas9
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLG1-puro-sgULK1-1

    Plasmid

    #109004

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ULK1-sgRNA #1 (ULK1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    mU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPTG-GG-sg2+sg3

    Plasmid

    #127199

    Purpose

    Plasmid expressing sgRNA2 (TACCACATTTGTAGAGGTT) & sgRNA3 (CAATGTATCTTATCATGTC) as polycistronic tRNA-gRNA from single U6 promoter

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    tRNA-gRNA
    

    Use

    Empty sgrna plasmid

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. sgp27#1

    Plasmid

    #102759

    Purpose

    Expresses sgRNA targeting mouse p27

    Depositor

    Jin Chen

    Article

    Song et al Oncogene. 2017 Jun 5. doi: 10.1038/onc.2

    Insert

    p27 (Cdkn1b Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge