We narrowed to 19,149 results for: tec

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pCH4 (denAsCas12a-KRAB lenti)

Plasmid

#217331

Purpose

Lentiviral expression of denAsCas12a-KRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

denAsCas12a

Use

CRISPR and Lentiviral

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Mutation

D908A/E174R/S542R/K548R

Promoter

UCOE-SFFV

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAB-EKARev-NLS

Plasmid

#118476

Purpose

ddRFP-based single-color ERK biosensor targeted to the nucleus.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-EKARev-NLS

Tags

6xHIS, Nuclear localization signal (NLS), T7 tag …

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-BE4max-NRRH

Plasmid

#136918

Purpose

Expresses BE4max-NRRH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

BE4max-NRRH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pSL3927 (pDonor(plasmid)_Pse)

Plasmid

#200905

Purpose

Encodes a mini-transposon derived from Pseudoalteromonas Tn7016,with a CmrR cargo, as well as a primer binding site for deep sequencing purposes at pTarget. Total transposon size = 1,087 bp.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

Mini Tn7016 (pTarget NGS)

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pv6_CBX7_Chromo_TAF3_Phd

Plasmid

#179400

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_Phd

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX7_Chromo - TAF3_Phd

Tags

AviTag and EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-BE4max-NRTH

Plasmid

#136919

Purpose

Expresses BE4max-NRTH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

BE4max-NRTH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pJT371 PB ITR and Cp36 attD pEF Puro-2a-mCherry donor

Plasmid

#184951

Purpose

Donor for both PiggyBac and Cp36 Recombinase

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

PuroR, P2A, mCherry

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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CABE-T3.1

Plasmid

#193267

Purpose

Expression of CABE-T3.1 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CABE-T3.1

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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SMC2 CRISPR

Plasmid

#140649

Purpose

SMC2 tagging CRISPR

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

SMC2 (SMC2 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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vDip-C: pAAV-Pcp2-mGreenLantern-KASH

Plasmid

#207613

Purpose

vDip-C AAV vector with mouse Pcp2 (also known as L7) promoter driving green fluorophore mGreenLantern with a KASH nuclear membrane tag to isolate cell nuclei of cerebellar Purkinje cells

Depositor

Longzhi Tan

Article

Tan et al Science. 2023 Sep 8;381(6662):1112-1119.

Insert

mGreenLantern

Use

AAV

Tags

KASH

Promoter

mouse Pcp2 promoter (L7-6)

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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KRas FAK biosensor

Plasmid

#78301

Purpose

FRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cells

Depositor

Yingxiao Wang

Article

Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

Insert

KRas FAK biosensor

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
pLKO.1P shSOD1

Plasmid

#102975

Purpose

Suppression of SOD1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shSOD1 (SOD1 Human)

Use

Lentiviral and RNAi

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-mSOG-FRB-IRES-mCherry

Plasmid

#136602

Purpose

Encodes FRB tagged with the N-terminal fragment of split-miniSOG (mSOG1-94).

Depositor

John Ngo

Article

Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

Insert

N-mSOG-FRB

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a-LCB1_v1.3-PDGFR-T2A-mCherry (pZYW048)

Plasmid

#194231

Purpose

Lentiviral expression vector expressing LCB1 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

EF1a-LCB1-PDGFR TMD-T2A-mCherry

Use

Lentiviral

Promoter

EF1a

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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rnLbCas12a-PE2

Plasmid

#213746

Purpose

For circular RNA-mediated prime editor using rnLbCas12a-PE2 in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

rnLbCas12a-M-MLV RT∆RNase H

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

D156R, H759A, R1138A in LbCas12a

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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CaTCH BC1

Plasmid

#154195

Purpose

Lentiviral CaTCH barcoding plasmid harboring a single barcode cassette (BC1)

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Insert

CaTCH BC1

Use

Lentiviral; Catch barcode labeling

Expression

Mammalian

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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CaTCH_empty-backbone

Plasmid

#157746

Purpose

Lentiviral CaTCH barcoding plasmid harboring an emtpy cloning site to insert a CaTCH barcoding library or individual barcode sequences

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Type

Empty backbone

Use

Lentiviral; Catch barcode cloning

Expression

Mammalian

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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HypaCas9

Plasmid

#138557

Purpose

Expresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

HypaCas9

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

N692A, M694A, Q695A, H698A

Promoter

EFS

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-ms-Sox17

Plasmid

#50781

Purpose

retroviral expression of mouse Sox17

Depositor

Shinya Yamanaka

Article

Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

Insert

Sox17 (Sox17 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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[pSK467] pLJC5 HA-Depdc5

Plasmid

#109343

Purpose

Lenti-viral expression of HA-tagged wildtype Depdc5

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

HA

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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