We narrowed to 26,296 results for: Vit

1. pET-FLAG-eSpCas9

   Plasmid

   #126769

   Purpose

   Expression of increased fidelity eSpCas9 in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A

   Promoter

   T7

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)MBII-85-3'ss mut

   Plasmid

   #67644

   Purpose

   MBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 3' splice site was mutated to consensus sequences.

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

   Insert

   MBII85 snoRNA
   

   Expression

   Mammalian

   Mutation

   3' splice site muattion

   Available Since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR-GFP-Patronin CC 638–1457

   Plasmid

   #59051

   Purpose

   for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   Patronin (Patronin Fly)
   

   Tags

   eGFP

   Expression

   Bacterial and Insect

   Available Since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pENTR-GFP-Patronin CC 776–1457

   Plasmid

   #59052

   Purpose

   for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   Patronin (Patronin Fly)
   

   Tags

   eGFP

   Expression

   Bacterial and Insect

   Available Since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pKDsgRNA-trmI

   Plasmid

   #89955

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting trmI.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   trmI gRNA
   

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-4T-1-Shp2 SH2s

   Plasmid

   #67575

   Purpose

   Expresses GST-tagged Shp2 SH2 domains in bacteria

   Depositor

   Graeme Carnegie

   Article

   Burmeister et al J Biol Chem. 2015 May 8;290(19):12058-67

   Insert

   Src homology 2 domain-containing phosphatase 2 SH2s
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Deleted amino acids 219-597

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pSUPER.retro.puro-shMePCE

   Plasmid

   #113536

   Purpose

   Retroviral vector designed to knock down MePCE.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   shMePCE (MEPCE Human)
   

   Use

   Retroviral

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. AB.pCCL.sin.cPPT.U6.miR-24-Decoy.hPGK.GFP.WPRE

   Plasmid

   #46602

   Depositor

   Brian Brown

   Article

   Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

   Insert

   miR-24 Decoy (Mir24-1 Human, Mouse)
   

   Use

   Lentiviral

   Promoter

   hU6

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

9. AB.pCCL.sin.cPPT.GFP.miR-370-5p.sensor.PGK.dNGFR.WPRE

   Plasmid

   #85854

   Purpose

   Sensor Plasmid

   Depositor

   Brian Brown

   Article

   Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

   Insert

   miR-370-5p Sensor (MIR370 Human)
   

   Use

   Lentiviral

   Promoter

   PGK

   Available Since

   Jan. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWJPrP5

    Plasmid

    #22106

    Depositor

    Susan Lindquist

    Article

    Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

    Insert

    mouse PrP ORF insert (Prnp Mouse)
    

    Use

    Construction of targeting vector

    Mutation

    mutated codon 177 (D to N)

    Available Since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pKDsgRNA-pfkB

    Plasmid

    #102284

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting pfkB.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    pfkB gRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. vsv-Sgo1 N61I-GFP

    Plasmid

    #108497

    Purpose

    expression of vsv-Sgo1 N61I-EGFP (no PP2A binding)

    Depositor

    Susanne Lens

    Article

    Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

    Insert

    Shugoshin 1 (SGO1 Human)
    

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    no PP2A binding N61I

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET15b+PCNA3 R112C/T180C

    Plasmid

    #66182

    Purpose

    Expresses the R112C/T180C mutant of Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 3
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C, T180C

    Promoter

    T7 promoter

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET15b+PCNA2 E105C/L171C

    Plasmid

    #66178

    Purpose

    Expresses the E105C/L171C mutant of Sulfolobus solfataricus PCNA2

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 2
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E105C, L171C

    Promoter

    T7 promoter

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKDsgRNA-xylA

    Plasmid

    #89964

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting xylA.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    xylA gRNA
    

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKDsgRNA-lpoB

    Plasmid

    #89959

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting lpoB.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    lpoB gRNA
    

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Ef1a-DIO-PBG-WPRE-hGH

    Plasmid

    #74287

    Purpose

    pAAV vector to express PBG (chimeric Glycoprotein) in a Cre-dependent manner

    Depositor

    Edward Callaway

    Article

    Kim et al Neuron. 2015 Dec 16;88(6):1253-67. doi:

    Insert

    PBG (chimeric rabies Glycoprotein)
    

    Use

    AAV

    Promoter

    Ef1a

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHL718

    Plasmid

    #62826

    Purpose

    Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::mCherry::T1 terminator + ColE1

    Depositor

    Han Lim

    Article

    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

    Inserts

    gfp
    mCherry
    

    Use

    Synthetic Biology

    Tags

    ipex leader and ompC leader

    Expression

    Bacterial

    Promoter

    pLlacO-1 and pLtetO-1

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

19. PRAS40 (mouse) shRNA

    Plasmid

    #15672

    Depositor

    John Lawrence

    Article

    Wang et al J Biol Chem. 2007 Jul 6. 282(27):20036-4

    Insert

    PRAS40 shRNA (Akt1s1 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Sept. 24, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pHD Btl-pYtag(Scarlet) ScarlessDsRed

    Plasmid

    #244937

    Purpose

    Vector for tagging Breathless (Btl) with Scarlet-based pYtag biosensor. DsRed marker.

    Depositor

    Jared Toettcher

    Article

    Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

    Insert

    mScarlet-tagged pYtag system (3xITAM P2A mScarlet-ZtSH2)
    

    Use

    Endogenous tagging

    Available Since

    Nov. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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