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459 pME-etv2

Plasmid

#49002

Purpose

full length coding sequence for zebrafish etv2 gene in gateway middle entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ets variant gene 2 (etsrp Zebrafish)

Use

Gateway middle entry

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcnj10a-IRES-EGFP

Plasmid

#164947

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj10a and EGFP

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj10a (kcnj10a Zebrafish)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Promoter

actinb (actb2) promoter

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pIBA2-SpyTag-MBP

Plasmid

#107422

Purpose

Control construct for SpyCatcher experiments. Encodes MBP with N-terminal SpyTag, including OmpA signal peptide for periplasmic targeting.

Depositor

Jack Leo

Article

Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

Insert

Maltose-binding protein (MalE)

Tags

OmpA signal peptide, SpyTag, and StrepII

Expression

Bacterial

Promoter

Tet

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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KHBD00433

Plasmid

#34538

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG9745 (D1 Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA6 MCV LTco S147A

Plasmid

#112192

Purpose

Expression of MCPyV LT mutant (Ser 147 to Ala)

Depositor

Patrick Moore

Article

Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

Insert

MCPyV LT codon optimized

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pDx_mScarlet-I-SYFP2

Plasmid

#189761

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet-I-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-I-SYFP2

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe-Tet-off-puro-mCherry-PA(wt)-Vav2

Plasmid

#91873

Purpose

Photo-activatable/light sensitive Vav2

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
VAV2 DH/PH/ZF (Vav2 Mouse)

Tags

mCherry

Expression

Bacterial and Mammalian

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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T7-His-GFP-Arp11

Plasmid

#51402

Purpose

expression of GFP-tagged mouse Arp11 under T7 promoter

Depositor

Trina Schroer

Article

Eckley et al Mol Biol Cell. 2003 Jul;14(7):2645-54. E

Insert

Arp11 (Actr10 Mouse)

Tags

GFP and His

Expression

Bacterial

Promoter

T7

Available Since

March 5, 2014

Availability

Academic Institutions and Nonprofits only
PNXGW

Plasmid

#48256

Purpose

Split-GFP assay in plants

Depositor

Wolf Frommer

Article

Kim et al Plant Cell. 2009 Apr;21(4):1305-23. doi:

Type

Empty backbone

Use

Binary gateway vector

Tags

6xHIS and YFP aa1-172

Promoter

35S

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
pMpGWB415

Plasmid

#68680

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Nuclear localization signal-tagged Citrine (Citri…

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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p3E-FAPP1

Plasmid

#109555

Purpose

Multisite gateway vector for 3' tagging with the PH domain from Human pleckstrin homology domain containing A3 (PLEKHA3). Binds PtdIns4P

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

the PH domain from Human pleckstrin homology domain containing A3 (PLEKHA3).

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pPK1E-1+t

Plasmid

#48147

Purpose

Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1E

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pMT469

Plasmid

#139395

Purpose

Nanoluc placed on Output 1 of the 2in3out circuit (A001 and P002 design) with aTC and Bile Acid as inputs, AmpR, ErmR

Depositor

Christopher Voigt

Article

Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

Insert

Nanoluc placed on Output 1 of the 2in3out circuit (A001 and P002 design) with aTC and Bile Acid as inputs

Use

Synthetic Biology

Promoter

aTC and BA inducible promoters

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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PCXGW

Plasmid

#48257

Purpose

Split-GFP assay in plants

Depositor

Wolf Frommer

Article

Kim et al Plant Cell. 2009 Apr;21(4):1305-23. doi:

Type

Empty backbone

Use

Binary gateway vector

Tags

6xHIS and CFP aa155-238

Promoter

35S

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
pTorPE-GEX-GECO1

Plasmid

#32464

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

GEX-GECO1.0

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

Expression

Bacterial

Mutation

GCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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pFETCh_MBD1_iso1

Plasmid

#86263

Purpose

Donor vector for 3' FLAG tag of human MBD1_iso1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

MBD1_iso1 homology arms (MBD1 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLSU4mg10

Plasmid

#126079

Purpose

Plant transformation vector for stable expression of mitochondria targeted GFP1-10 in plant cells

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Binary

Tags

GFP1-10

Expression

Plant

Promoter

'long' CaMV35S

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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p63RhoGEF580-mVenus-N1

Plasmid

#84338

Purpose

Expresses p63 (580 a.a.) tagged with mVenus

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2016 Nov 11;6:36825. doi: 10.10

Insert

p63RhoGEF (580 aa) (ARHGEF25 Human)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO MycLAP-STIL ∆CC

Plasmid

#80268

Purpose

mammalian expression plasmid for MycLAP-STIL coiled-coil deletion

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

STIL (STIL Human)

Tags

Myc-GFP

Expression

Mammalian

Mutation

missing conserved coiled-coil (amino acids 721-74…

Promoter

CMV

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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KHBD00409

Plasmid

#34517

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG5249 (Blimp-1 Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pET28a-P5CR

Plasmid

#110295

Purpose

Expresses pyrroline-5-carboxylate reductase in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Pyrroline-5-carboxylate Reductase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pME-hygro-hPGAP3-3HA

Plasmid

#50373

Purpose

Express C-terminally triple HA tagged human PGAP3 in mammmalian cells

Depositor

Reika Watanabe

Article

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

PGAP3 post-GPI attachment to proteins 3 (PGAP3 Human)

Tags

3x HA

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
KHBD00671

Plasmid

#39722

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6312 (Rfx Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
pDEST myc MOV10 K530A

Plasmid

#51719

Purpose

plasmid expresses myc-tagged MOV10 K530A

Depositor

Markus Landthaler

Article

Gregersen et al Mol Cell. 2014 May 22;54(4):573-85. doi:

Insert

MOV10 K530A (MOV10 Human)

Tags

myc

Expression

Mammalian

Mutation

K530A

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pInducer21 Flag-Sesn2

Plasmid

#61829

Purpose

Dox inducible lentivirial expression of Flag-Sesn2 (mouse)

Depositor

Ming Li

Article

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn2 (Sesn2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
KHBD00340

Plasmid

#34463

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8573 (su(Hw) Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
Rat SERCA3 (pMT2)

Plasmid

#75185

Purpose

Mammalian expression of ATP2A3

Depositor

Jonathan Lytton, Gary Shull

Article

Burk et al J Biol Chem. 1989 Nov 5;264(31):18561-8.

Insert

ATP2A3 (Atp2a3 Rat)

Expression

Mammalian

Promoter

adenovirus major late promoter

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV.U6gLacZ.hSyn.H2B.RFP

Plasmid

#170369

Purpose

Negative control plasmid used for Crispr/cas9 based disruption. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B RFP

Use

AAV

Promoter

human Synapsin

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pESUMO-hRORg2

Plasmid

#170329

Purpose

Expresses RORg isoform 2 with N-terminal HisSUMO tag in bacteria

Depositor

Patrick Griffin

Article

Strutzenberg et al J Mol Biol. 2021 Sep 20;433(22):167258.

Insert

RORG isoform 2 (RORC Human)

Tags

HisSUMO

Expression

Bacterial

Mutation

codon optimized

Promoter

T7

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCD5-ss-D/bovine/France/5920/2014-HEFwtED-GCN4-sfGFP-ST

Plasmid

#175017

Purpose

Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

Depositor

Robert de Vries

Article

Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

Insert

OK-HEFwtED

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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