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pPB-CAG-mNanog-mut-pgk-hph

Plasmid

#74914

Purpose

Mammalian expression of mNanog (Y42E, K43T)

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Nanog (Nanog Mouse)

Expression

Mammalian

Mutation

K43T of Nanog protein and Y42E of Nanog homeodoma…

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-mIFITM3-deltaY20

Plasmid

#58459

Purpose

Expresses murine IFITM3-deltaY20 with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (Ifitm3 Mouse)

Tags

myc

Expression

Mammalian

Mutation

Tyrosine 20 deleted

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-myc-mIFITM3-Y20A

Plasmid

#58460

Purpose

Expresses murine IFITM3-Y20A with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (Ifitm3 Mouse)

Tags

myc

Expression

Mammalian

Mutation

Tyrosine 20 to alanine

Promoter

CMV IE

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only
Nanog-t2A-mCherry

Plasmid

#127541

Purpose

Expresses the transactivation domain of mouse Nanog and mCherry via t2A linker.

Depositor

Jennifer Mitchell

Article

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Insert

Nanog (Nanog Mouse)

Expression

Mammalian

Mutation

Codes for the last 110 a.a of Nanog

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shNuf2

Plasmid

#160961

Purpose

Nuf2 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nuf2 shRNA (Nuf2 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCas9n-Nanog-L

Plasmid

#122302

Purpose

Expresses sgRNA targeting mouse Nanog and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Nanog (Nanog Synthetic)

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-mIFITM3-Y20,27A

Plasmid

#58465

Purpose

Expresses murine IFITM3-Y20,27A with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (Ifitm3 Mouse)

Tags

myc

Expression

Mammalian

Mutation

Tyrosines 20 and 27 to alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-myc-mIFITM3-Y27A

Plasmid

#58464

Purpose

Expresses murine IFITM3-Y27A with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (Ifitm3 Mouse)

Tags

myc

Expression

Mammalian

Mutation

Tyrosine 27 to alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-H136T

Plasmid

#58413

Purpose

Expresses murine IFITM3-H136T with an N-terminal HA tag in mammalian cells (adds a possible glycosylation site)

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Histidine 236 to Threonine

Promoter

CMV IE

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-HA-CAD

Plasmid

#46238

Purpose

For expression of HA and FLAG-tagged murine CAD in mammalian cells

Depositor

Brendan Manning

Article

Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

Insert

carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Zmynd8+exon minimal CMV pCDNA5

Plasmid

#212079

Purpose

LR vector for integration of Zmynd8+exon(48nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Zmynd8+exon(48nt) (Zmynd8 Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-PB-SRT-tdTomato

Plasmid

#154889

Purpose

piggyBac self-reporting transposon with tdTomato marker in AAV backbone

Depositor

Joseph Dougherty, Rob Mitra

Article

Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

Insert

piggyBac SRT

Use

Adenoviral

Expression

Mammalian

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 p37deltaSEP

Plasmid

#113501

Purpose

Bacterial expression of GST-tagged p37 lacking the SEP domain

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Tags

GST-PreScission protease site

Expression

Bacterial

Mutation

delta 140-206

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-3XFLAG-Vcp

Plasmid

#123600

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

valosin containing protein (Vcp Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pHD-T2A-split-intein-Gal4[21-881]-C-int

Plasmid

#199204

Purpose

To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[21-881]C-int

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[21-881]-C-int

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS mSynCAM1

Plasmid

#115496

Purpose

expression of mouse full-length SynCAM 1, CAG promoter suited for neuronal expression

Depositor

Thomas Biederer

Article

Fogel et al J Neurosci. 2007 Nov 14;27(46):12516-30.

Insert

Cadm1 (Cadm1 Mouse)

Expression

Mammalian

Promoter

chicken beta-actin

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only
aav-tnt-actn2-gfp

Plasmid

#165034

Purpose

AAV-based delivery of ACTN2-GFP into cardiomyocytes in vivo

Depositor

William Pu

Article

Guo et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

Insert

ACTN2 (Actn2 Mouse)

Use

AAV

Tags

GFP

Promoter

cTNT

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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HA-mRIN2 dRA

Plasmid

#108941

Purpose

Mammalian expression of mRIN2 dRA

Depositor

Guido Serini

Article

Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

Insert

Ras and Rab interactor 2 (Rin2 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Deletion of RA domain (aa 751-836)

Promoter

CMV

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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HA-mRIN2 dRHVPS9

Plasmid

#108942

Purpose

Mammalian expression of mRIN2 dRHVPS9

Depositor

Guido Serini

Article

Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

Insert

Ras and Rab interactor 2 (Rin2 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Deletion of RHVPS9 domain (aa 455-738)

Promoter

CMV

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Nanog_2

Plasmid

#174871

Purpose

CRISPR vector for generating Nanog STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Nanog (Nanog Synthetic)

Use

CRISPR

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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