169,885 results
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pAAV-EF1a-ArgiNLS-EGFP
Plasmid#220594PurposeCre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein.DepositorInsertEGFP
UseAAVTagsArgiNLSExpressionMammalianPromoterEF1aAvailable SinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Spike-deltaC18 KP.2
Plasmid#223273PurposeEncodes SARS-CoV-2 spike KP.2 for pseudovirus productionDepositorInsertSARS-CoV-2 Spike KP.2 (S Severe acute respiratory syndrome coronavirus 2)
ExpressionMammalianMutationSee Depositor Comments.PromoterCAGAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-Lmo2
Plasmid#60666PurposePiggyBac expression vector encoding murine Lmo2 downstream of a doxycycline inducible promoterDepositorAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_RACK1:1-317
Plasmid#211110PurposeMammalian expression of full-length human RACK1. N-terminal HiBiT tag.DepositorInsertRACK1:M1-R317 (RACK1 Human)
TagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable SinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
BirA in pET28a (w400-2)
Plasmid#26624DepositorInsertBirA
TagsHisExpressionBacterialAvailable SinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
C8orf55-CD4d3+4-bio
Plasmid#73126PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding C8orf55 with rat CD4d3+4-bioDepositorInsertC8orf55 (THEM6 Human)
Tagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianPromoterCMVAvailable SinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAM5505
Plasmid#132664PurposepRL623-based helper plasmid for conjugations, with RSF1010 mobA geneDepositorInsertMobA
ExpressionBacterialMutationplease see depositor comments belowPromoterp1,p3Available SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Anillin
Plasmid#68027PurposeFull length Anillin rendered RNAi-resistant) in mammalian expression vector pEGFP-C1DepositorInsertAnillin (ANLN Human)
TagseGFPExpressionMammalianMutationsilent mutations at 798 5'-TGCC TCTTTGAATAAA…PromoterCMVAvailable SinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-NFIA.1-SOX9 (bi-directional promoter)
Plasmid#182306PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expressionUsePiggybacTagsNone and T2A-mycNLS-mTagBFP2ExpressionMammalianPromoterTRE3G-Bi-directionalAvailable SinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-Tet3
Plasmid#60940PurposeExpresses Tet3 in mammalian cellsDepositorInsertTen-Eleven Translocation 3
TagsflagExpressionMammalianPromoterCMVAvailable SinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSC-ISL1-T2A-LHX3
Plasmid#90215PurposeTo convert human skin fibroblasts into induced motor neurons (hiMN) in combination with NGN2, Sox11, FGF2 and two small molecules, forskolin and dorsomorphin.DepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF1713
Plasmid#142832PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMLS360
Plasmid#73687PurposePunc-47::2xNLS-FLP-D5 Expression vectorDepositorInsertPunc-47::2xNLS-FLP-D5::let-858 3'-UTR
UseFlp/frtExpressionWormPromoterPunc-47Available SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQCasTns(Ptr)-array 8
Plasmid#175580PurposeExpression of CRISPR-associated transposasesDepositorInsertPtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(8 CRISPR array)
ExpressionBacterialMutationNOAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbS8k-RFP
Plasmid#35276DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterBADAvailable SinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
IVT-nCas9(H840A)
Plasmid#207455Purposein vitro transcription of mRNADepositorInsertH840A
ExpressionBacterialAvailable SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFB2G
Plasmid#195290PurposeBicistronic HSV-TK and mTagBFP resistance for positive/negative selectionDepositorInsertHSV-TK 2A mTagBFP
ExpressionMammalianPromoterPGKAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-OTI-TCRA_p2_TCRB
Plasmid#131033PurposeExpresses OT-I TCRA linked to TCRB with the P2A self-cleaving peptideDepositorArticleAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-CD19-Fc(DAPA)-AviTag-6xHis
Plasmid#156714PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertCD19 (CD19 Human)
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(EF1a no GFP) mnuctdTomato
Plasmid#172342PurposemnuctdTomatoDepositorInsertsmonomeric nuclear Tomato
HygR
UseLentiviral and Synthetic BiologyTagsSV40 NLSExpressionMammalianPromoterEF1alphaAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K27
Plasmid#22902DepositorInsertUbiquitin C (UBC Human)
TagsHAExpressionMammalianMutationK27 only, all other lysines mutated to argininesAvailable SinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_MMP-9-HA
Plasmid#121172PurposeExpression of mouse MMP9DepositorInsertMMP9 (matrix metallopeptidase 9) (Mmp9 Mouse)
TagsHAExpressionMammalianMutationSee depositor comments belowPromoterCMV immediate/earlyAvailable SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNLS-TetR-GFP
Plasmid#103838PurposeFlourescent marker for tetO arraysDepositorAvailable SinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
1XDR4
Plasmid#32614DepositorInsert1XDR4
ExpressionMammalianAvailable SinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLYS5-SDHB-Flag
Plasmid#50055PurposeExpression of human SDHB in mammalian cellsDepositorAvailable SinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBSKΔB- CAG-rtTA2sM2-IRES-tTSkid-IRES-Neo
Plasmid#62346PurposeExpresses rtTA2sM2 and tTSkid in mammalian cellsDepositorInsertrtTA2sM2-IRES-tTSkid
ExpressionMammalianPromoterCAGAvailable SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJFRC-nSyb-IVS-PhiC31
Plasmid#133868PurposeReplace the Nsyb promoter to express PhiC31 under the control of any promoter of interest.DepositorInsertPhiC31 Recombinase (int )
ExpressionInsectAvailable SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJK369
Plasmid#73594PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitopeDepositorInsertdelta-12 fatty acid acetylenase
TagsFLAG tagExpressionYeastPromoterGAL10Available SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJMP1356
Plasmid#119276Purposefor cloning new sgRNAsDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only
