165,307 results

1. pFastBac-M13-6His-KIF1C-GFP

   Plasmid

   #130975

   Purpose

   Expression of 6His-KIF1C-GFP in insect cells for protein purification.

   Depositor

   Anne Straube

   Article

   Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

   Insert

   KIF1C-GFP (KIF1C Human)
   

   Tags

   6His and GFP

   Expression

   Insect

   Mutation

   5 silent mutations that make this construct RNAi …

   Promoter

   Polyhedrin

   Available Since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3puro-EGFP-GAPDH

   Plasmid

   #225475

   Purpose

   Express mEGFP-fusion protein of GAPDH

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   GAPDH (GAPDH Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP(N3)-PP1beta

   Plasmid

   #44223

   Depositor

   Laura Trinkle-Mulcahy, Angus Lamond

   Article

   Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

   Insert

   PP1B (PPP1CB Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiCRISPRv2_cGAS_gRNA_3

   Plasmid

   #235531

   Purpose

   gRNA against human cGAS

   Depositor

   Nicolas Manel

   Article

   Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

   Insert

   cGAS (CGAS Human)
   

   Use

   Lentiviral

   Available Since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGMC00030 (aka pRC0707)

   Plasmid

   #209673

   Purpose

   Lentiviral vector expressing PP7-P65-HSF1

   Depositor

   Raj Chari

   Article

   Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

   Insert

   PP7-P65-HSF1 (HSF1, RELA Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

   Plasmid

   #134661

   Purpose

   Expresses EGFP-tagged synaptic vesicle protein

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   vesicle-associated membrane protein 2 (VAMP2 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-2E2-HA-mEGFP

   Plasmid

   #129595

   Purpose

   The encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.

   Depositor

   Tim Stasevich

   Article

   Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

   Insert

   Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. HSA3_His

   Plasmid

   #202765

   Purpose

   human serum albumin design HSA3, in pET29b with C-terminal 6His-tag

   Depositor

   Sarel Fleishman

   Article

   Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

   Insert

   HSA3
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSMT3_SRSF1

   Plasmid

   #201056

   Purpose

   Express sumo-tagged human SRSF1

   Depositor

   Jun Zhang

   Article

   Fargason et al Elife. 2023 Mar 2;12:e84412. doi: 10.755

   Insert

   SRSF1 (SRSF1 Human)
   

   Tags

   sumo

   Expression

   Bacterial

   Mutation

   none

   Available Since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti6.3/TO/CD9-GFP

    Plasmid

    #104402

    Purpose

    Expresses CD9 with mEmerald tag (NOT GFP) at the N terminal

    Depositor

    Jens Gruber

    Article

    Boker et al Mol Ther. 2018 Feb 7;26(2):634-647. doi:

    Insert

    CD9 (CD9 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Promoter

    CMV

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHelper_ShCAST_sgRNA

    Plasmid

    #127921

    Purpose

    Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
    

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. RfxCas13d-backbone

    Plasmid

    #165078

    Purpose

    Expression of RfxCas13d sgRNAs from a U6 promoter

    Depositor

    Ling-Ling Chen

    Article

    Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

    Insert

    Scaffold gRNA
    

    Expression

    Mammalian

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6-PspCas13b-GEMSgRNA2

    Plasmid

    #204768

    Purpose

    pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

    Depositor

    Kate Meyer

    Article

    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    guide RNA targeting GEMS m6A sensor mRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6-mir451 EF1Alpha-puro-T2A-BFP

    Plasmid

    #164792

    Purpose

    Express miR-451 under the U6 promoter in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    microRNA 451a (MIR451A Human)
    puro-T2A-BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1Alpha and U6

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRE-Tn5-160

    Plasmid

    #118540

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    TagBFP2
    

    Use

    Minitn5 delivery vector

    Expression

    Bacterial

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTHSSe_46

    Plasmid

    #109244

    Purpose

    pSC101 var4 BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Expression

    Bacterial

    Promoter

    PJ23105

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Ef1a-DIO-mCherry-WPRE-pA

    Plasmid

    #37083

    Purpose

    Cre-dependent expression of mCherry

    Depositor

    Bernardo Sabatini

    Article

    Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

    Insert

    mCherry
    

    Use

    AAV and Cre/Lox; Cre-on

    Promoter

    EF1a

    Available Since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. TALED_Right-RNR2-1397N

    Plasmid

    #183897

    Purpose

    Expresses TALED_Right-RNR2-1397N in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

    Insert

    TALED for RNR2 mutation
    

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. TALED_Left-RNR2-1397C-AD

    Plasmid

    #183896

    Purpose

    Expresses TALED_Left-RNR2-1397C-AD in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

    Insert

    TALED for RNR2 mutation
    

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJFRC150-20XUAS-IVS-Flp1::PEST

    Plasmid

    #32132

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

    Insert

    Flippase1::PEST
    

    Tags

    PEST

    Expression

    Insect

    Mutation

    Aspartic Acid at Amino Acid 5

    Available Since

    Sept. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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21. 35S-GAL4BD-GWY/pAM

    Plasmid

    #201233

    Purpose

    Used to produce and express in planta N-terminal fusion proteins with the GAL4 BD

    Depositor

    Michael Ayliffe

    Article

    Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

    Insert

    35S-GAL4BD-GWY
    

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. Tol2_B-act_dCas9-Vp64-T2A-Citrine

    Plasmid

    #200248

    Purpose

    Zebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesis

    Depositor

    Tatjana Sauka-Spengler

    Article

    Pelea et al bioRxiv 2023.05.08.539738

    Insert

    dCas9-Vp64-T2A-Citrine
    

    Use

    Zebrafish expression

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. p20StAD

    Plasmid

    #190268

    Purpose

    Constitutively expresses a protein of interest fused to a GAL4 transcription activating domain. Targeted integration to genomic locus YPRC-delta15, Chromosome XVI.

    Depositor

    Assaf Mosquna

    Article

    Zimran et al ACS Synth Biol. 2022 Aug 19;11(8):2880-2

    Type

    Empty backbone

    Use

    Synthetic Biology; Yeast genome-integrating vector

    Tags

    GAL4-AD

    Promoter

    TDH3

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCytERM-mTagBFP2

    Plasmid

    #184940

    Purpose

    ER Visualization (OSER assay) Expresses mTagBFP2 in fusion with N-terminal part of p450

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    CytERM-mTagBFP2
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. pEBTet-SNAP-Cep72

    Plasmid

    #136819

    Purpose

    Mammalian expression of the centrosomal protein Cep72 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep72 (CEP72 Synthetic, Human)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pTetOFF SNCA-A53T

    Plasmid

    #215372

    Purpose

    All-in-one lentiviral construct for the dox repressible expression of the A53T mutant of alpha-synuclein.

    Depositor

    Franz-Ulrich Hartl

    Article

    Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

    Insert

    SNCA (SNCA Human)
    

    Expression

    Mammalian

    Mutation

    Ala53Thr

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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27. pcDNA5/FRT/TO_H2B-HaloTag11-T2A-EGFP

    Plasmid

    #175522

    Purpose

    Nuclear expression of HaloTag11 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag11
    

    Expression

    Mammalian

    Mutation

    HaloTag11 = HaloTag7-M175W

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. pSOX11-donor

    Plasmid

    #176360

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Barbara Stranger, Kevin White

    Insert

    SOX11 (SOX11 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Available Since

    Nov. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. pRham-CALM1

    Plasmid

    #182500

    Purpose

    Bacterial expression of the Homo sapiens calmodulin, CALM1

    Depositor

    Dan Mulvihill

    Article

    Baker et al FEBS Lett. 2022 Jan 31. doi: 10.1002/187

    Insert

    CALM1 (CALM1 Human)
    

    Expression

    Bacterial

    Promoter

    rhamnose

    Available Since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. pminiTol2

    Plasmid

    #31829

    Depositor

    Stephen Ekker

    Article

    Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

    Type

    Empty backbone

    Use

    Cloning vector

    Available Since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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