We narrowed to 8,677 results for: sgrna

1. pYPQAT30

   Plasmid

   #223402

   Purpose

   T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 and the sgRNA was driven by separate ZmUbi1; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-ZmUbi-gRNA scaffold 2.0-tRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQAT14

   Plasmid

   #223386

   Purpose

   T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and sgRNA was by AtU3; BASTA for plant selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-hA3A-Y130F-SpCas9-D10A-UGI-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. KL094 - pTRE3G-Flag-Halo-CTCF_WT-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

   Plasmid

   #232930

   Purpose

   Vector to introduce a constitutive rtta3G cassette and a DOX-inducible Flag-Halo-mCTCF WT, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycin selection.

   Depositor

   Danny Reinberg

   Article

   Lucero et al bioRxiv, March 19, 2025

   Insert

   Flag-Halo-CTCF WT
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEIF3D-RNAgateDel-sgEIF3D

   Plasmid

   #222137

   Purpose

   EIF3D variant overexpression vector with sgRNA targeting eIF3D

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   eIF3D (EIF3D Human)
   

   Use

   Lentiviral

   Tags

   BFP-P2A

   Mutation

   V291-F306 deletion

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEIF3D-S528D-S529D-sgEIF3D

   Plasmid

   #222127

   Purpose

   EIF3D variant overexpression vector with sgRNA targeting eIF3D

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   eIF3D (EIF3D Human)
   

   Use

   Lentiviral

   Tags

   BFP-P2A

   Mutation

   S528D, S529D

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEIF3D-NtermDel-CtermDel-sgEIF3D

   Plasmid

   #222131

   Purpose

   EIF3D variant overexpression vector with sgRNA targeting eIF3D

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   eIF3D (EIF3D Human)
   

   Use

   Lentiviral

   Tags

   BFP-P2A

   Mutation

   161 + 527 truncation

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEIF3D-NtermDel-sgEIF3D

   Plasmid

   #222123

   Purpose

   EIF3D variant overexpression vector with sgRNA targeting eIF3D

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   eIF3D (EIF3D Human)
   

   Use

   Lentiviral

   Tags

   BFP-P2A

   Mutation

   S161 truncation

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEIF3D-a11helixDel-sgEIF3D

   Plasmid

   #222135

   Purpose

   EIF3D variant overexpression vector with sgRNA targeting eIF3D

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   eIF3D (EIF3D Human)
   

   Use

   Lentiviral

   Tags

   BFP-P2A

   Mutation

   T317E, N320E, H321A

   Available Since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEIF3D-CtermDel-sgEIF3D

   Plasmid

   #222125

   Purpose

   EIF3D variant overexpression vector with sgRNA targeting eIF3D

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   eIF3D (EIF3D Human)
   

   Use

   Lentiviral

   Tags

   BFP-P2A

   Mutation

   F527 truncation

   Available Since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEIF3D-a5helixDel-sgEIF3D

    Plasmid

    #222133

    Purpose

    EIF3D variant overexpression vector with sgRNA targeting eIF3D

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    eIF3D (EIF3D Human)
    

    Use

    Lentiviral

    Tags

    BFP-P2A

    Mutation

    D249Q, V262I, Y263A

    Available Since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEIF3D-S528N-S529N-sgEIF3D

    Plasmid

    #222129

    Purpose

    EIF3D variant overexpression vector with sgRNA targeting eIF3D

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    eIF3D (EIF3D Human)
    

    Use

    Lentiviral

    Tags

    BFP-P2A

    Mutation

    S528N, S529N

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOTTC1706 - pAAV mGrid1 390F gRNA EF1a EGFP-KASH

    Plasmid

    #131683

    Purpose

    An adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a guide RNA for mGrid1

    Depositor

    Christopher Richie

    Article

    Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

    Inserts

    EGFP-KASH
    SpCas9 sgRNA vs mouse GRID1
    

    Use

    AAV

    Tags

    KASH

    Promoter

    EF1a and mU6

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CMV-dSa VP64 Hbb

    Plasmid

    #99693

    Purpose

    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Hbb promoter, vector allows for activation of mouse Hbb, can be packaged and delivered as AAV

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9 and gRNA targeting Hbb (gRNA: GGGGTAAGGGGAGCAAGGTC) (Hbb Mouse, Synthetic)
    

    Use

    AAV and CRISPR

    Tags

    VP64

    Mutation

    dead Cas9

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Sha3Cas9

    Plasmid

    #192136

    Purpose

    Expresses Sha3Cas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wang et al Adv Sci (Weinh). 2022 Feb;9(4):e2104789.

    Insert

    Sha3Cas9
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTij_sg_mmu_Med6_C_terminal

    Plasmid

    #197869

    Purpose

    CRISPR/Cas9/sgRNA plasmid for cutting Med6 C terminal and building MED6-mEmerald/Halo knock-in mESCs

    Depositor

    James Zhe Liu

    Article

    Dong et al bioRxiv 2022.10.26.513917

    Insert

    Med6 (Med6 Mouse)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CMV-Hsp1-Hsp2Cas9-Y-P2A-puro

    Plasmid

    #192132

    Purpose

    Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

    Depositor

    Yongming Wang

    Article

    Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

    Insert

    Hsp1-Hsp2Cas9-Y
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    Hsp1-Hsp2Cas9 (Y446A)

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiU6-hIL1RN gRNA_1-MS2-Puro

    Plasmid

    #192677

    Purpose

    Lentiviral expression of sgRNA targeting hIL1RN promoter to activate human IL1RN transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human IL1RN activating gRNA #1 (IL1RN Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-dora[B]_rescue_construct

    Plasmid

    #190638

    Purpose

    Puromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Tags

    3xHA

    Expression

    Insect

    Mutation

    Changed threonine 295 to methionine

    Promoter

    Actin-5c

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. HA-dora[A]_rescue_construct

    Plasmid

    #190640

    Purpose

    Puromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Tags

    3xHA

    Expression

    Insect

    Mutation

    Truncated to contain amino acids 1-945

    Promoter

    Actin-5c

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFUGW-scrambled

    Plasmid

    #183455

    Purpose

    This control vector contains a scrambled version of the targeting sequence used in the pFUGW-shRIIα construct

    Depositor

    Matthew Gold

    Article

    Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

    Insert

    scrambled sgRNA
    

    Use

    Lentiviral

    Promoter

    shRNA: H1 / gene: ubiquitin

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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