12,469 results

1. Venus-BimEL-4E-pEGFP-C1

   Plasmid

   #166742

   Purpose

   Express Venus fused to the N-terminus of the Bcl-2 family protein, BimEL with mutation in BH3 domain to disrupt binding anti-apoptotic proteins

   Depositor

   David Andrews

   Article

   Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

   Insert

   BimEL-4E (Bcl2l11 Mouse)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   4 hydrophobic residues in the BH3 region mutated …

   Promoter

   CMV

   Available since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Venus-BimL-4E-pEGFP-C1

   Plasmid

   #166741

   Purpose

   Express Venus fused to the N-terminus of the Bcl-2 family protein, BimL with mutation in BH3 domain to disrupt binding anti-apoptotic proteins

   Depositor

   David Andrews

   Article

   Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

   Insert

   BimL-4E (Bcl2l11 Mouse)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   4 hydrophobic residues in the BH3 region mutated …

   Promoter

   CMV

   Available since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Venus-BimEL(Bid)-pEGFP-C1

   Plasmid

   #166759

   Purpose

   To examine effect of swapping the BH3 region in full length BimEL with that of Bid

   Depositor

   David Andrews

   Article

   Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

   Insert

   BimEL(Bid) (Bcl2l11, Bid Mouse)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   BH3 region removed and inserted BH3 of mouse Bid …

   Promoter

   CMV

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Venus-BimEL(Bad)-pEGFP-C1

   Plasmid

   #166758

   Purpose

   To examine effect of swapping the BH3 region in full length BimEL with that of Bad

   Depositor

   David Andrews

   Article

   Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

   Insert

   BimEL(Mus musculus)-(Bad BH3) (Bcl2l11, BAD Mouse, Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   BH3 region removed and inserted BH3 of human Bad …

   Promoter

   CMV

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Venus-tBid-2E-pEGFP-C1

   Plasmid

   #166747

   Purpose

   Express Venus fused to the N-terminus of the Bcl-2 family protein, tBid with mutation to 2 positions in the BH3 domain

   Depositor

   David Andrews

   Article

   Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

   Insert

   tBid-2E (Bid Mouse)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   2 hydrophobic residues in the BH3 region mutated …

   Promoter

   CMV

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRNATU6.1 HDAC3 shRNA1

   Plasmid

   #112249

   Purpose

   HDAC3 KD

   Depositor

   Simone Di Giovanni

   Insert

   shRNA against mouse HDAC3 (Hdac3 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRNATU6.1 HDAC3 shRNA4neg

   Plasmid

   #112273

   Purpose

   shRNA negative control

   Depositor

   Simone Di Giovanni

   Insert

   shRNA negative control
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLV-EF1a-KIF5AR280H-HA-IRES-Puro

   Plasmid

   #166953

   Purpose

   Lentiviral plasmid expressing HA-tagged KIF5A R280H protein with IRES-Puro from the EF1a promoter

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   KIF5A-R280H (Kif5a Mouse)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   KIF5A-R280H

   Promoter

   eF1a

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Myc-KLC1 delta Tail

   Plasmid

   #166965

   Purpose

   Expresses Myc-tagged KLC1 protein (1-495 aa) in pcDNA3.1

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   KLC1 (Klc1 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   KLC1 (1-495aa)

   Promoter

   CMV

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FLAG-KIF5AR280H

    Plasmid

    #166957

    Purpose

    Expresses FLAG-tagged KIF5A R280H protein in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KIF5A-R280H (Kif5a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    KIF5A-R280H

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. KLC1N343S-Myc

    Plasmid

    #166963

    Purpose

    Expresses Myc-tagged KLC1 N343S protein in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KLC1-N343S (Klc1 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    KLC1-N343S

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-CAG-GFP-mirMecp2

    Plasmid

    #163704

    Purpose

    pAAV plasmid to induce expression of micro-RNA targeted against mouse Mecp2 and EGFP

    Depositor

    Michisuke Yuzaki

    Article

    Ito-Ishida et al J Neurosci. 2020 Nov 4;40(45):8746-8766.

    Insert

    mirMecp2 and EGFP (Mecp2 Mouse, Aequorea victoria)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. px330 WAPL gRNA

    Plasmid

    #165595

    Purpose

    Insertion of WAPL degrom

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    WAPL (Wapl Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. px330 BRM gRNA

    Plasmid

    #165586

    Purpose

    Insertion of BRM degron

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    BRM (Smarca4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GP821

    Plasmid

    #164552

    Purpose

    Expresses Smo-FLAG K434R

    Depositor

    Gregory Pazour

    Article

    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    Smoothened K434R (Smo Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    One of the three Lysines in loop three of Smoothe…

    Promoter

    GgCryD1

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMin-DHS-4D-GFP

    Plasmid

    #159653

    Purpose

    Reporter plasmid for the DHS-4D Otx2 enhancer element.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    DHS-4D
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TATA

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. KIF13B

    Plasmid

    #163907

    Purpose

    Full length mouse KIF13B for purification from insect cells

    Depositor

    Martin Loose

    Article

    Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    kif13b (Kif13b Mouse)
    

    Use

    Tags

    Cleavable 6 x HIS tag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. KIF13B 1-584

    Plasmid

    #163908

    Purpose

    Motile fragment of mouse KIF13B (aa 1-584) for purification from insect cells.

    Depositor

    Martin Loose

    Article

    Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    kif13b (Kif13b Mouse)
    

    Use

    Tags

    Cleavable 6 x HIS tag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mWasabi-ADAP1

    Plasmid

    #163906

    Purpose

    Full length mouse ADAP1 with N terminal fluorescent tag for purification from insect cells

    Depositor

    Martin Loose

    Article

    Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    ADAP1 (Adap1 Mouse)
    

    Use

    Tags

    mWasabi fluorescent tag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GP758

    Plasmid

    #164541

    Purpose

    Expresses Sstr3-FLAG-Ub

    Depositor

    Gregory Pazour

    Article

    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    Somatostatin receptor type 3 (Sstr3 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG-Ub

    Expression

    Mutation

    WT

    Promoter

    CMV

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Talin1-FERM

    Plasmid

    #110563

    Purpose

    bacterial expression of Talin 1

    Depositor

    Tina Izard

    Insert

    Talin 1 (Tln1 Mouse)
    

    Use

    Tags

    (His)8

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. GP826

    Plasmid

    #164551

    Purpose

    Expresses Smo-FLAG K444, 448R

    Depositor

    Gregory Pazour

    Article

    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    Smoothened K444, 448R (Smo Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Two of the three Lysines in loop three of Smoothe…

    Promoter

    GgCryD1

    Available since

    Feb. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GP825

    Plasmid

    #164550

    Purpose

    Expresses Smo-FLAG K434, 448R

    Depositor

    Gregory Pazour

    Article

    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    Smoothened K434, 448R (Smo Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Two of the three Lysines in loop three of Smoothe…

    Promoter

    GgCryD1

    Available since

    Feb. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GP823

    Plasmid

    #164554

    Purpose

    Expresses Smo-FLAG K448R

    Depositor

    Gregory Pazour

    Article

    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    Smoothened K448R (Smo Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    One of the three Lysines in loop three of Smoothe…

    Promoter

    GgCryD1

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GP822

    Plasmid

    #164553

    Purpose

    Expresses Smo-FLAG K444R

    Depositor

    Gregory Pazour

    Article

    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    Smoothened K444R (Smo Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    One of the three Lysines in loop three of Smoothe…

    Promoter

    GgCryD1

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. GP824

    Plasmid

    #164549

    Purpose

    Expresses Smo-FLAG K434, 444R

    Depositor

    Gregory Pazour

    Article

    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    Smoothened K434, 444R (Smo Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Two of the three Lysines in loop three of Smoothe…

    Promoter

    GgCryD1

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PD30

    Plasmid

    #164543

    Purpose

    Expresses Drd1-Ub

    Depositor

    Gregory Pazour

    Article

    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    Dopamine receptor D1 (Drd1 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG-Ub

    Expression

    Mutation

    WT

    Promoter

    CMV

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GP940

    Plasmid

    #164539

    Purpose

    Expresses SmoPi-FLAG-Ub

    Depositor

    Gregory Pazour

    Article

    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    Smoothened R455A (Smo Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG-Ub

    Expression

    Mutation

    R455A

    Promoter

    CMV

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. GP938

    Plasmid

    #164538

    Purpose

    Expresses SmoPi-FLAG

    Depositor

    Gregory Pazour

    Article

    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    Smoothened R455A (Smo Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    R455A

    Promoter

    CMV

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GP807

    Plasmid

    #164535

    Purpose

    Expresses Smo noK-FLAG-Ub noK

    Depositor

    Gregory Pazour

    Article

    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    Smoothened noK (Smo Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG_Ub noK

    Expression

    Mutation

    All 21 cytoplasmic Lysines of Smoothened and all…

    Promoter

    CMV

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge