We narrowed to 26,401 results for: Vit

1. pET28a-T5-ASG_ClpXP

   Plasmid

   #153299

   Purpose

   Expresses acoustic sensor genes or ClpXP-sensitive gas vesicles in E. coli Nissle 1917

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

   Insert

   ASG
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCASP-SptP120-K27-HilA

   Plasmid

   #153328

   Purpose

   Triggers secretion of SptP120 fused to the K27 DARPin from Salmonella upon induction with arabinose

   Depositor

   Josef Penninger

   Article

   Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

   Insert

   SptP120-K27 DARPin
   

   Use

   Salmonella expression

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKDsgRNA-rpsL

   Plasmid

   #89953

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rpsL.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   rpsL gRNA
   

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. IBMc097

   Plasmid

   #161945

   Purpose

   Contains Type IIS restriction site free elements for IBM staging vectors. Staging plasmid for IBM on mCherry

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB1C3(BsmBI-)-BsaI-mCherry*(27-218)-BsaI
   

   Expression

   Bacterial

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)MBII-85-5'/3'ss mut

   Plasmid

   #67645

   Purpose

   MBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 5' and 3' splice sites were mutated to consensus sequences.

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

   Insert

   MBII85 snoRNA
   

   Expression

   Mammalian

   Mutation

   5' and 3' splice sites mutations

   Available Since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-h-mmPtch1-B

   Plasmid

   #120888

   Purpose

   Expresses Ptch1-B for function assays

   Depositor

   Philip Beachy

   Article

   Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

   Insert

   Ptch1 (Ptch1 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPK1E-1+t

   Plasmid

   #48147

   Purpose

   Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1E

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX6P1‐Afu‐rnhBΔPIP

   Plasmid

   #108696

   Purpose

   For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with C-terminal PIP box deletion

   Depositor

   Andrew Jackson, Martin Reijns

   Article

   Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

   Insert

   rnhB
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   C-terminal truncation (7aa) removing PIP box

   Promoter

   tac

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX6P1‐Afu‐rnhB D101N

   Plasmid

   #108695

   Purpose

   For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with D101N catalytic site mutation

   Depositor

   Andrew Jackson, Martin Reijns

   Article

   Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

   Insert

   rnhB
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   D101N catalytic site mutation

   Promoter

   tac

   Available Since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOmpW

    Plasmid

    #112121

    Purpose

    Bacterial expression of outer membrane protein W (OmpW), wild type

    Depositor

    Karen Fleming

    Article

    Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

    Insert

    OmpW (ompW E. coli)
    

    Expression

    Bacterial

    Mutation

    N-terminal signal sequence not included and repla…

    Promoter

    T7

    Available Since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-hSyntenin2b

    Plasmid

    #89437

    Purpose

    mammalian expression of EGFP tagged human Syntenin2b

    Depositor

    Fritz Rathjen

    Article

    Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

    Insert

    SDCBP2 (SDCBP2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    *see below

    Promoter

    CMV

    Available Since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. SaABE8e-dimer

    Plasmid

    #138501

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-nSaCas9
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. enAsABE8e-dimer

    Plasmid

    #138507

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-dAsCas12a (En)
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-3xFLAG-Puro PAK5 E144K

    Plasmid

    #167193

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 E144K

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 E144K
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-3xFLAG-Puro PAK5 M173I

    Plasmid

    #167192

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 M173I

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 M173I
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFLAG-mISG15-4EHP

    Plasmid

    #17354

    Depositor

    Dong-Er Zhang

    Article

    Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

    Insert

    mISG15-4EHP (EIF4E2 Human, Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

17. FLAG-SLC30A10-N43H

    Plasmid

    #82344

    Purpose

    Expresses FLAG-tagged SLC30A10-N43H in mammalian cells

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

    Insert

    SLC30A10 (SLC30A10 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N43H

    Promoter

    CMV

    Available Since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. FLAG-SLC30A10-D248A

    Plasmid

    #82346

    Purpose

    Expresses FLAG-tagged SLC30A10-D248A in mammalian cells

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

    Insert

    SLC30A10 (SLC30A10 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D248A

    Promoter

    CMV

    Available Since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLL7.0: Venus-oLID-CAAX (from KRas4B)

    Plasmid

    #60412

    Purpose

    Mammalian Expression of Venus-oLID-CAAX

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    Venus-oLID-CAAX
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5 FRT/TO MycLAP-STIL ∆CC

    Plasmid

    #80268

    Purpose

    mammalian expression plasmid for MycLAP-STIL coiled-coil deletion

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    STIL (STIL Human)
    

    Tags

    Myc-GFP

    Expression

    Mammalian

    Mutation

    missing conserved coiled-coil (amino acids 721-74…

    Promoter

    CMV

    Available Since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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