We narrowed to 8,809 results for: CAG

1. paavCAG-JxON-pre-mGRASP-mCerulean

   Plasmid

   #51903

   Purpose

   JxOn-pre

   Depositor

   Jinhyun Kim

   Article

   Druckmann et al Neuron. 2014 Feb 5;81(3):629-40. doi: 10

   Insert

   presynaptic mGRASP
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Jan. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-CAG-DIO-VARNAM2-Kv2.1PR

   Plasmid

   #195533

   Purpose

   Cre-dependent expression of a red fluorescent, negative response-polarity voltage indicator; soma-targeted

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   VARNAM2-Kv2.1 proximal restriction sequence
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   VARNAM SI linker

   Promoter

   CAG

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-CAG-Brainbow4.2-PGK-HygTK

   Plasmid

   #214126

   Purpose

   Gene expression by Piggybac transposase in mammalian cells

   Depositor

   Sho Yoshimatsu

   Insert

   Brainbow
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized for mammalian cells

   Promoter

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLL3.7-CAG-hMGT-Ef1a-puro

   Plasmid

   #172395

   Purpose

   Lentiviral expression vector for human direct cardiac reprogramming

   Depositor

   Li Qian

   Article

   Garbutt et al STAR Protoc. 2020 Jun 19;1(1). doi: 10.1

   Insert

   Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAVS1-SA-neo-CAGGS-PE2-2A-GFP

   Plasmid

   #180014

   Purpose

   Targeting vector for knocking in constitutively expressed PE2-2A-GFP into the human AAVS1 locus

   Depositor

   Dirk Hockemeyer

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   PE2-2A-GFP
   

   Use

   CRISPR; Human genome engineering

   Tags

   GFP

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAGGS-Flag-hsDicer (D1320A/D1709A)

   Plasmid

   #41585

   Depositor

   Phil Sharp

   Article

   Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

   Insert

   hsDicer (DICER1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D1320A/D1709A

   Promoter

   CMV

   Available since

   Feb. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

7. AAV-CAG-LSL-mCherry-shPtbp1

   Plasmid

   #178591

   Purpose

   Cre-dependent expression of mCherry and a shRNA against Ptbp1 under the constitutive promoter

   Depositor

   Chun-Li Zhang

   Article

   Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

   Insert

   miRE-Ptbp1
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV-CAG-FLPX-rc [ChrimsonR-tdTomato]

   Plasmid

   #130909

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the CAG promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Has Service

   AAV8

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato (codon diversified version)

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   CAG

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKS004 - pCAGGS-3XFLAG-CTCF-eGFP

   Plasmid

   #156438

   Purpose

   For transient expression of mouse CTCF tagged with eGFP

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   mouse CTCF (Ctcf Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-CAG-fDIO-VARNAM2-Kv2.1PR

    Plasmid

    #195536

    Purpose

    Flp recombinase-dependent red fluorescent, negative response-polarity voltage indicator; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    VARNAM2-Kv2.1 proximal restriction sequence
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    VARNAM SI linker

    Promoter

    CAG

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pB-CAG-FLEX-F14F15-BFP-(mCherry)'

    Plasmid

    #201831

    Purpose

    piggyBac transposon plasmid for making Cre and Flpo reporter cell lines using piggyBac transposase

    Depositor

    Ian Wickersham

    Article

    Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Inserts

    CAG
    BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAGG>nls-hCas9-nls-GFP

    Plasmid

    #99141

    Purpose

    Cas9-GFP fusion protein under the regulation of chicken beta-actin promoter

    Depositor

    Marianne Bronner

    Article

    Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

    Insert

    Cas9-GFP
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Chicken beta actin (CAG)

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAVS1-Puro CAG CLTX-CAR

    Plasmid

    #157743

    Purpose

    knock CLTX-CAR into AAVS1 safe harbor locus for constitutive expression

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    chlorotoxin containing chimeric antigen receptor targeting glioblastoma
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBRPyCAG-Mettl3-dsRed-IRES-puro

    Plasmid

    #61516

    Purpose

    Constitutive expression of murine wild-type Mettl3

    Depositor

    Jacob Hanna

    Article

    Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

    Insert

    Mettl3 (Mettl3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGG

    Available since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-1BPNLS-Cas9-1BPNLS-2AGFP

    Plasmid

    #87109

    Purpose

    BPNLS-Cas9-BPNLS-2AGFP expression plasmid

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    1BPNLS-Cas9-1BPNLS-2A-EGFP
    

    Use

    CRISPR

    Tags

    3xFLAG (on Cas9)

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-CFP-SaCas9-HF (without sgRNA)

    Plasmid

    #134470

    Purpose

    Human expression vector for SaCas9-HF variant.

    Depositor

    Zongli Zheng

    Article

    Tan et al Proc Natl Acad Sci U S A. 2019 Sep 30. p

    Insert

    Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
    

    Use

    CRISPR

    Tags

    SV40NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    R245A, N413A, N419A, R654A

    Promoter

    CAG

    Available since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pD2529-CAG-Flag-mouse TGFb1

    Plasmid

    #180260

    Purpose

    Mouse full length TGFβ1 expression

    Depositor

    Timothy Springer

    Article

    Jiang et al Cancer Immunol Res. 2022 Feb 18. pii: 68

    Insert

    TGFβ1 (Tgfb1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPB-CAG-empty-pgk-hph

    Plasmid

    #48753

    Purpose

    PiggyBac backbone, CAG promoter, Hygromycin selection

    Depositor

    Austin Smith

    Article

    Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

    Type

    Empty backbone

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pAAV-CAG-FLEX-rc [Chronos-tdTomato]

    Plasmid

    #84484

    Purpose

    AAV-mediated expression of Chronos-tdTomato under the CAG promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CAGGS-AsCpf1-2A-GFP-U6-INS-sgRNA

    Plasmid

    #159283

    Purpose

    A multicistronic vector with CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) targeting the human INS locus (sgINS).

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

    Inserts

    Human INS sgRNA
    Human INS guide RNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV2/1-CAG-Flex-FlpO-WPRE

    Plasmid

    #171690

    Purpose

    Cre-dependent expression of Flp-recombinase

    Depositor

    Tobias Bonhoeffer, Mark Huebener, Tobias Rose

    Article

    Bauer et al Neuron. 2021 Aug 4;109(15):2457-2468.e12

    Insert

    Flip-recombinase
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEN-CAG-mRFP-PCNA pc2729

    Plasmid

    #166040

    Purpose

    Expresses mRFP tagged human PCNA in mammalian cells.

    Depositor

    Cristina Cardoso

    Article

    Heinz et al Nucleic Acids Res. 2018 Jul 6;46(12):611

    Insert

    Proliferating Cell Nuclear Antigen (PCNA Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-CAG-FLEx-iSeroSnFR-EnhancedExport

    Plasmid

    #129180

    Purpose

    Fluorescent reporter for serotonin imaging (conditional + membrane localization tag with enhanced export sequence)

    Depositor

    Lin Tian

    Article

    Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

    Insert

    iSeroSnFR
    

    Use

    AAV

    Tags

    Ig-kappa leader, Myc, and PDGFR + enhanced ER exp…

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAGGS-mTagBFP2-T2A-iCre-ERT2

    Plasmid

    #128168

    Purpose

    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAPGSTMA N-terminus)

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    mTagBFP2-T2A-iCreERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAGGS SARS-CoV-2 BA.2.75 Spike

    Plasmid

    #190012

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 BA.2.75 Spike

    Depositor

    Marceline Côté

    Article

    Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

    Insert

    SARS-CoV-2 BA.2.75 Spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains the BA.2 mutations (T19I, LPPA24S, G142D…

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available since

    Sept. 7, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pCAG-Myc-GRM1-IRES-CyRFP1

    Plasmid

    #195355

    Purpose

    mGluR1-IRES-cyRFP1 under the control of the pCAG promoter

    Depositor

    Brenda Bloodgood

    Article

    Campbell et al Cell Rep. 2023 Jan 31;42(1):111943. doi:

    Insert

    GRM1-IRES-CyRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAGGS-PDGFRb559-562del-IRES2-EGFP

    Plasmid

    #204355

    Purpose

    Expresses a mutant PDGFRb gene (559-562 deletion).

    Depositor

    Hirofumi Nakatomi

    Article

    Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

    Insert

    Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)
    

    Use

    Tags

    Expression

    Mutation

    559-562 deletion

    Promoter

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAGGS SARS-CoV-2 D614G Spike

    Plasmid

    #185692

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 D614G Spike

    Depositor

    Marceline Côté

    Article

    Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

    Insert

    SARS-CoV-2 D614G Spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains the following mutation: D614G

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available since

    June 8, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. AAV-CAG-dDIO-MCS--4x6T

    Plasmid

    #196422

    Purpose

    Empty vector for astrocyte-selective Dre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Type

    Empty backbone

    Use

    AAV; Dre/rox; astrocyte-selective

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PD2529-CAG-Flag-mouse TGFb2

    Plasmid

    #180261

    Purpose

    Mouse full length TGFβ2 expression

    Depositor

    Timothy Springer

    Article

    Jiang et al Cancer Immunol Res. 2022 Feb 18. pii: 68

    Insert

    TGFβ2 (Tgfb2 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. YA1532: pAAV_ CAG-FLEX-paQuasAr3

    Plasmid

    #107702

    Purpose

    in vivo voltage imaging

    Depositor

    Adam Cohen

    Article

    Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

    Insert

    paQuasAr3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPyCAG-tagBFP-3xNLS-IRES-Hph

    Plasmid

    #183608

    Purpose

    Mammalian expression vector for tagBFP-3xNLS from the CAG promoter. Confers hygromycin B resistance.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    tagBFP-3xNLS
    

    Use

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCAGGS-ACE2-his expression plasmid

    Plasmid

    #158089

    Purpose

    Expresses ACE2 in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    ACE2 (ACE2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP

    Plasmid

    #114111

    Purpose

    fusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Prakash et al J Neurosci Res. 2018 Aug 28. doi: 10.100

    Insert

    Gaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-CAG-miRFP713-P2A-GFP

    Plasmid

    #197163

    Purpose

    Expresses the protein of miRFP713-P2A-GFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    miRFP713-P2A-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCAG-Golden-Gate-Esp3I-Destination

    Plasmid

    #51278

    Purpose

    CAG promoter-driven expression vector suitable for ligation of multiple inserts by Golden Gate Cloning using Esp3I

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. AAVS1-Pur-CAG-hM3Dq-mCherry

    Plasmid

    #80948

    Purpose

    human AAVS1 site targeting donor plasmid for knock-in hM3Dq-mCherry expression cassette driven by CAG promoter

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

    Insert

    hM3Dq-mCherry
    

    Use

    Tags

    mcherry fusion

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_3

    Plasmid

    #178049

    Purpose

    CAG tdGFP Lrig1 shRNAmir-3 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Lrig1 shRNAmir
    

    Use

    Cre/Lox and RNAi; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_2

    Plasmid

    #178048

    Purpose

    CAG tdGFP Lrig1 shRNAmir-2 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Lrig1 shRNAmir
    

    Use

    Cre/Lox and RNAi; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_1

    Plasmid

    #178047

    Purpose

    CAG tdGFP Lrig1 shRNAmir-1 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Lrig1 shRNAmir
    

    Use

    Cre/Lox and RNAi; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS

    Plasmid

    #199252

    Purpose

    Expression construct encoding the high specificity eSpCas9(1.1)-D10A nickase

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Insert

    High specificity eSpCas9(1.1)-D10A nickase
    

    Use

    CRISPR

    Tags

    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A K848A K1003A R1060A

    Promoter

    CAG promoter

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pD2529-CAG-Flag-mouse TGFb3

    Plasmid

    #180262

    Purpose

    Mouse full length TGFβ3 expression

    Depositor

    Timothy Springer

    Article

    Jiang et al Cancer Immunol Res. 2022 Feb 18. pii: 68

    Insert

    TGFβ3 (Tgfb3 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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43. pAAV-CAG-FRT-Rev-3xGFP

    Plasmid

    #191204

    Purpose

    FlpO dependent GFP expression

    Depositor

    Janelia Viral Tools

    Article

    Winnubst et al Cell. 2019 Sep 19;179(1):268-281.e13. do

    Insert

    3X GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. AAV-CAG-flex-lck-smV5

    Plasmid

    #196423

    Purpose

    Cre-dependent AAV expression of membrane-targeted V5 spaghetti monster reporter; no cell-type specificity

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    Lck-smV5
    

    Use

    AAV and Cre/Lox

    Tags

    Lck

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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45. pZ P TetON-3XFLAG-tdT CAGG-m2rtTA v2

    Plasmid

    #112668

    Purpose

    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression of your protein of interest.

    Depositor

    Catherine Verfaillie

    Article

    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

    Insert

    tdT
    

    Use

    AAV; Donor plasmid for recombinase-mediated casse…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. AAVS1-Pur-CAG-hM4Di-mCherry

    Plasmid

    #80947

    Purpose

    human AAVS1 site targeting donor plasmid for knock-in hM4Di-mCherry expression cassette driven by CAG promoter

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

    Insert

    hM4Di-mCherry
    

    Use

    Tags

    mcherry fusion

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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47. MMLV-CAG-G_oT7pol-IRES-BSD

    Plasmid

    #172369

    Purpose

    Generation of oT7pol-expressing stable cell lines, resistant to the antibiotic Blasticidin

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    oT7pol + BSD
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pAAV-CAG-EBFP2-P2A-EGFP

    Plasmid

    #184938

    Purpose

    Expresses blue fluorescent protein EBFP2 and EGFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    EBFP2-P2A-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCAG-mCherry-FKBP-GAP(ARHGAP29)

    Plasmid

    #222632

    Purpose

    Rho inactivation using rapamycin

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    mCherry-FKBP-GAP(ARHGAP29) (ARHGAP29 Human)
    

    Use

    Tags

    mCherry-FKBP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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50. MMLV-CAG-SADB19_oG-IRES-Puro

    Plasmid

    #172367

    Purpose

    Generation of stable cell lines expressing the rabies optimized glycoprotein (oG), resistant to the antibiotic Puromycin

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    oG + Puro
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge