We narrowed to 12,031 results for: cel.2

1. pv6_CBX7_Chromo_TAF3_Phd

   Plasmid

   #179400

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_Phd

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX7_Chromo - TAF3_Phd
   

   Use

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available Since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFLAG-Tetherin

   Plasmid

   #41070

   Depositor

   Paul Spearman

   Article

   Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

   Insert

   Tetherin (BST2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pv6_dPC_Chromo_TAF3_Phd

   Plasmid

   #179403

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of dPC_Chromo - TAF3_Phd

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   dPC_Chromo - TAF3_Phd
   

   Use

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEX-PML B-box1 C4C4

   Plasmid

   #185640

   Purpose

   expression of GST-tagged mutant PML B-box1 H155C/H161C domain in bacterial cells

   Depositor

   Irina Bezsonova

   Article

   Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

   Insert

   promyelocytic leukemia protein (PML Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   H155C H161C

   Promoter

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pv6_CBX7_2xChromo-W35A

   Plasmid

   #179397

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_2xChromo(W35A)

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX7_2xChromo(W35A)
   

   Use

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Mutation

   Chromodomain W35A

   Promoter

   CAGGS

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pv6_CBX1_2xChromo-W42A

   Plasmid

   #179394

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo(W42A)

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX1_2xChromo(W42A)
   

   Use

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Mutation

   Chromodomain W42A

   Promoter

   CAGGS

   Available Since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pv7_MBD1_MBD_noEGFP

   Plasmid

   #179406

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   MBD1_MBD
   

   Use

   Tags

   AviTag

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available Since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pv7_CBX1_2xChromo_noEGFP

   Plasmid

   #179395

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo_noEGFP

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX1_2xChromo_noEGFP
   

   Use

   Tags

   AviTag

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available Since

   Aug. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pv6_MBD1_MBD-R22A

   Plasmid

   #179405

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD(R22A)

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   MBD1_MBD(R22A)
   

   Use

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Mutation

   MBD R22A

   Promoter

   CAGGS

   Available Since

   Aug. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAGGS-Omicron.BA.5-RBD

    Plasmid

    #213478

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.BA.5 variant

    Depositor

    Priyamvada Acharya

    Article

    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

    Insert

    pCAGGS/Omicron.BA.5 RBD (S )
    

    Use

    Tags

    6 His Tag

    Expression

    Mammalian

    Mutation

    G339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…

    Promoter

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pv9_TAF3_2xPHD-13XL-BASU

    Plasmid

    #179414

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of TAF3.2xPHD-13XL-BASU

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    TAF3.2xPHD-13XL-BASU
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pv9_CBX1_2xChromo-13XL-BASU

    Plasmid

    #179412

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1.2xChromo-13XL-BASU

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX1.2xChromo-13XL-BASU
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pv9_CBX7_2xChromo-13XL-BASU

    Plasmid

    #179413

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7.2xChromo-13XL-BASU

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX7.2xChromo-13XL-BASU
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pv9_MBD1_MBD-WT-13XL-BASU

    Plasmid

    #179416

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1.MBD-WT-13XL-BASU

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    MBD1.MBD-WT-13XL-BASU
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFLAG-4S Tetherin

    Plasmid

    #41073

    Depositor

    Paul Spearman

    Article

    Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

    Insert

    Tetherin (BST2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S74, S84, 137S, and 144S

    Promoter

    CMV

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pFLAG-137.144S Tetherin

    Plasmid

    #41072

    Depositor

    Paul Spearman

    Article

    Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

    Insert

    Tetherin (BST2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    137S, 144S

    Promoter

    CMV

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pFLAG-74.84S Tetherin

    Plasmid

    #41071

    Depositor

    Paul Spearman

    Article

    Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

    Insert

    Tetherin (BST2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    74S, 84S

    Promoter

    CMV

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pRK5-Myc-PSK2 (K57A)

    Plasmid

    #197118

    Purpose

    Expression of kinase-defective MYC-tagged PSK2 (K57A) / TAOK1 (K57A) in mammalian cells

    Depositor

    Jonathan Morris

    Article

    Zihni et al J Biol Chem. 2006 Mar 17;281(11):7317-23

    Insert

    TAOK1 (K57A) (TAOK1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Changed K 57 to A

    Promoter

    SV40

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pv6_CBX7_Chromo-W35A_TAF3_Phd

    Plasmid

    #179401

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo(W35A) - TAF3_Phd

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX7_Chromo(W35A) - TAF3_Phd
    

    Use

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    Chromodomain W35A

    Promoter

    CAGGS

    Available Since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSMT3-FBF2 SS/R

    Plasmid

    #166976

    Purpose

    Expresses FBF-2 with SS/R mutations in repeat 5 in E. coli for purification and crystallization studies

    Depositor

    Traci Hall

    Article

    Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

    Insert

    FBF-2 (fbf-2 Nematode)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    FBF-2 PUM RNA binding domain; amino acids 164-575…

    Promoter

    T7

    Available Since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSMT3-FBF2 AQ/Y

    Plasmid

    #166975

    Purpose

    Expresses FBF-2 with AQ/Y mutations in repeat 5 in E. coli for purification and crystallization studies

    Depositor

    Traci Hall

    Article

    Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

    Insert

    FBF-2 (fbf-2 Nematode)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    FBF-2 PUM RNA binding domain; amino acids 164-575…

    Promoter

    T7

    Available Since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Dendra2-NS3

    Plasmid

    #238004

    Purpose

    Expresses NS3 in mammalian/mouse cell line

    Depositor

    Partha Mondal

    Insert

    NS3
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-UbC

    Plasmid

    #62806

    Purpose

    To express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.

    Depositor

    Jae Lee, Pantelis Tsoulfas

    Article

    Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

    Type

    Empty backbone

    Use

    AAV; Eucaryotic

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available Since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMTgR2

    Plasmid

    #107286

    Purpose

    Plasmid can be used for Drosophila S2 driven secretion of extracellular part of human interferon-_ receptor 2.

    Depositor

    Bohdan Schneider

    Article

    Mikulecky et al Acta Crystallogr D Struct Biol. 2016 Sep

    Insert

    Interferon gamma receptor 2 (IFNGR2 Human)
    

    Use

    Tags

    CATCATCACCATCACCATGA

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein promoter

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pv9_MBD1_MBD-R22A-13XL-BASU

    Plasmid

    #179417

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1.MBD-R22A-13XL-BASU

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    MBD1.MBD-R22A-13XL-BASU
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pv6_CBX7_Chromo_TAF3_Phd-DW890/891AA

    Plasmid

    #179402

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_Phd(DW890/891AA)

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX7_Chromo - TAF3_Phd(DW890/891AA)
    

    Use

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    Phd domain DW890/891AA

    Promoter

    CAGGS

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSMT3-FBF2 AS/Y

    Plasmid

    #166974

    Purpose

    Expresses FBF-2 with AS/Y mutations in repeat 5 in E. coli for purification and crystallization studies

    Depositor

    Traci Hall

    Article

    Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

    Insert

    FBF-2 (fbf-2 Nematode)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    FBF-2 PUM RNA binding domain; amino acids 164-575…

    Promoter

    T7

    Available Since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBScMXT + egl2

    Plasmid

    #16209

    Depositor

    Larry Salkoff

    Article

    Weinshenker et al J Neurosci. 1999 Nov 15. 19(22):9831-40.

    Insert

    K+-channel KCNQ (egl-2 Nematode)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available Since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

29. pcDNA3.1-IDH2-WT

    Plasmid

    #87926

    Purpose

    Expresses IDH2 (wild-type) in mammalian cells

    Depositor

    Reproducibility Project Cancer Biology

    Article

    Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

    Insert

    isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA 3.1-NbON10-NanoLuc

    Plasmid

    #198691

    Purpose

    Expresses anti-SARS-Cov-2 Nucleocapsid nanobody ON10 fused to NanoLuc in HEK293 cell culture

    Depositor

    Gualberto González Sapienza

    Article

    Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

    Insert

    anti-SARS-CoV-2 Nucleocapsid nanobody ON10
    

    Use

    Tags

    6xHis tag, IgK leader sequence, NanoLuc, and twin…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCMV6-AN-DDK_S433A POLI

    Plasmid

    #131235

    Purpose

    Mammalian expression of S433A DNA polymerase iota with an N-terminal FLAG-tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains a point mutation converting Pol iota ser…

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMV6-AN-DDK_S497A POLI

    Plasmid

    #131236

    Purpose

    Mammalian expression of S497A DNA polymerase iota with an N-terminal FLAG-tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains a point mutation converting Pol iota ser…

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV6-AN-DDK_S520A POLI

    Plasmid

    #131237

    Purpose

    Mammalian expression of S520A DNA polymerase iota with an N-terminal FLAG-tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains a point mutation converting Pol iota ser…

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV6-AN-DDK_S580A POLI

    Plasmid

    #131238

    Purpose

    Mammalian expression of S580A DNA polymerase iota with an N-terminal FLAG-tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains a point mutation converting Pol iota ser…

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV6-AN-DDK_3KA POLI

    Plasmid

    #131239

    Purpose

    Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A and K446A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains point mutations that convert lysine resi…

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJH23

    Plasmid

    #159314

    Purpose

    Punc-25 GFP::SYD-2

    Depositor

    Mei Zhen

    Article

    Yeh et al J Neurosci. 2005 Apr 13;25(15):3833-41.

    Insert

    syd-2 (syd-2 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    Punc-25

    Available Since

    Sept. 22, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pk335-CARGO-3mer-30kb-DSF

    Plasmid

    #227486

    Purpose

    3-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to 30kb Downstream Fgf5
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pk335-CARGO-3mer-35kb-DSF

    Plasmid

    #227492

    Purpose

    3-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to 35kb Downstream Fgf5
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pk335-CARGO-6mer-Fgf5Pro

    Plasmid

    #227479

    Purpose

    6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to Fgf5 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pk335-CARGO-5mer-Fgf5Pro

    Plasmid

    #227480

    Purpose

    5-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to Fgf5 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pk335-CARGO-4mer-Fgf5Pro

    Plasmid

    #227481

    Purpose

    4-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to Fgf5 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pk335-CARGO-4mer-PrPro

    Plasmid

    #227455

    Purpose

    4-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to Prdm8 promoter Upstream Prdm8
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pk335-CARGO-3mer-PrPro

    Plasmid

    #227456

    Purpose

    3-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to Prdm8 promoter Upstream Prdm8
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pk335-CARGO-3mer-36kb-USF

    Plasmid

    #227464

    Purpose

    3-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to 36kb Upstream Fgf5
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pcDNA3.1-IDH2-R172K

    Plasmid

    #87927

    Purpose

    Expresses IDH2 (R172K mutant) in mammalian cells

    Depositor

    Reproducibility Project Cancer Biology

    Article

    Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

    Insert

    isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    R172K

    Promoter

    CMV

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pAAV-hSyn-DIO {mCAR}off-{DTR-GFP}on-WPRE

    Plasmid

    #111395

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection), Cre-ON DTR-EGFP (diphtheria toxin receptor for cell ablation)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    mCAR (Cxadr Mouse)
    DTR-EGFP
    

    Use

    AAV

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pCMV6-AN-DDK_RKK/AAA POLI

    Plasmid

    #131240

    Purpose

    Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations R524A, K526A and K530A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains point mutations that convert arginine re…

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pk335-CARGO-6mer-70kb-DSF

    Plasmid

    #227498

    Purpose

    6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to 70kb Downstream Fgf5
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pk335-CARGO-6mer-73kb-DSF

    Plasmid

    #227499

    Purpose

    6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to 73kb Downstream Fgf5
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pk335-CARGO-6mer-26kb-DSF

    Plasmid

    #227482

    Purpose

    6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to 26kb Downstream Fgf5
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge