165,528 results

1. p5xATF6-GL3

   Plasmid

   #11976

   Purpose

   Reporter gene with five ATF6 sites that is highly sensitive to ER stress

   Depositor

   Ron Prywes

   Article

   Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2

   Insert

   5xATF6 binding site
   

   Tags

   Luc and c-fos promoter

   Expression

   Mammalian

   Available Since

   Aug. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSFFV-dNme2Cas9

   Plasmid

   #193190

   Purpose

   Lentiviral vector expressing nuclease-dead Cas9 from Neisseria meningitis (dNme2)

   Depositor

   Erik Sontheimer

   Article

   Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

   Insert

   dNme2Cas9
   

   Tags

   NLS and NLS-3xHA-NLS

   Expression

   Mammalian

   Mutation

   H588A, D16A

   Promoter

   SFFV

   Available Since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMRXIP TMEM41B-3xFLAG

   Plasmid

   #117415

   Purpose

   Expresses TMEM41B tagged with 3xFLAG in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

   Insert

   TMEM41B (TMEM41B Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a MDHM_YEAST

   Plasmid

   #204231

   Purpose

   Bacterial expression of yeast mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   MDHM_YEAST (MDH1 Budding Yeast)
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   Does not include transit peptide and includes a M…

   Promoter

   Lac

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pENTR5'-attP (pCM254)

   Plasmid

   #68311

   Purpose

   Gateway middle entry vector for full-length attP site for phiC31-mediated transgenesis.

   Depositor

   Christian Mosimann, Leonard Zon

   Article

   Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

   Insert

   attP
   

   Use

   Gateway entry vector

   Available Since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCS2+Paxillin-mKate

   Plasmid

   #105974

   Purpose

   in vitro transcription, paxillin coding sequence without stop codon-paxilin is a protein of focal adhesions

   Depositor

   Caren Norden

   Article

   Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

   Insert

   Paxillin
   

   Tags

   mKate

   Expression

   Bacterial

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ODD-Luciferase-pcDNA3

   Plasmid

   #18965

   Depositor

   William Kaelin

   Article

   Safran et al Proc Natl Acad Sci U S A. 2006 Jan 3. 10

   Insert

   ODD-Firefly Luciferase (HIF1A Human)
   

   Expression

   Mammalian

   Mutation

   HIF1a ODD sequence fused in frame to luciferase O…

   Available Since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP

   Plasmid

   #69908

   Purpose

   Expresses a GFP circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ZKSCAN1, GFP (ZKSCAN1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mEmerald-Nesprin3-C-18

   Plasmid

   #54203

   Purpose

   Localization: Nuclear Membrane , Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Nesprin3 (SYNE3 Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pCAG2-EGFP-C-IFT144

    Plasmid

    #218740

    Purpose

    Expresses C-terminally EGFP-tagged IFT144 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Insert

    IFT144 (WDR19 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRU294

    Plasmid

    #167689

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::Cas9i
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pT2/CMV-Luc/PGK-SB100X

    Plasmid

    #192869

    Purpose

    Carries luciferase under CMV promoter flanked with IR/DR elements. Also carries SB100X under PGK promoter outside of the IR/DR elements.

    Depositor

    David Largaespada, Maria Castro

    Article

    Calinescu et al J Vis Exp. 2015 Feb 22;(96). doi: 10.379

    Inserts

    luc
    SB100X
    

    Expression

    Mammalian

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLV-CBA-dt/PGK-CFTR-⌀

    Plasmid

    #163923

    Purpose

    Expresses human CFTR.

    Depositor

    John Engelhardt

    Article

    Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

    Insert

    CFTR (CFTR Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCX-cMyc

    Plasmid

    #19772

    Purpose

    Non-integrating (episomal) expression of mouse c-Myc

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Science. 2008 Nov 7. 322(5903):949-53.

    Insert

    c-Myc (Myc Mouse)
    

    Expression

    Mammalian

    Available Since

    Jan. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIRES_IBB-mCherry-IRES-HA-mAID-nanobody

    Plasmid

    #117720

    Purpose

    Expression of nuclear transfection marker IBB-mCherry and HA-tagged mAID-nanobody from one mRNA

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Inserts

    mAID-nanobody
    IBB-mCherry
    

    Tags

    importin-beta bining domain (IBB) and triple HA

    Expression

    Mammalian

    Mutation

    codon optimized mAID for murine expression

    Promoter

    CMV and None, IRES-driven

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Tad-CBE(TCA3)

    Plasmid

    #235064

    Purpose

    Expresses Tad-CBE(TCA3) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(TCA3)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, bpNLS and bpNLS

    Expression

    Mammalian

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRC-CMV-Rev1b

    Plasmid

    #204153

    Purpose

    Lentivirus helper plasmid coding for Rev

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    Rev
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-nanoHER2-SpyTag003

    Plasmid

    #216312

    Purpose

    Expresses nanoHER2-SpyTag003 in bacterial cells. nanoHER2 is an anti-HER2 nanobody.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    nanoHER2-SpyTag003
    

    Tags

    His6 tag and SpyTag003

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHIV-ODD-EGFP-IRES-dTomato

    Plasmid

    #87218

    Purpose

    lentiviral expression of ODD-EGFP

    Depositor

    Violaine Sée

    Article

    Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

    Insert

    HIF-1a ODD domain (HIF1A Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    ODD domain

    Promoter

    EF1

    Available Since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNK497

    Plasmid

    #219754

    Purpose

    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    

    Expression

    Plant

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge