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pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR

Plasmid

#172982

Purpose

control & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusion

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

control sgRNA

Use

CRISPR and Lentiviral

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-CBA-dt/PGK-CFTR-⌀

Plasmid

#163923

Purpose

Expresses human CFTR.

Depositor

John Engelhardt

Article

Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

Insert

CFTR (CFTR Human)

Use

Lentiviral

Expression

Mammalian

Mutation

WT

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_CD8alpha SNIPR

Plasmid

#188357

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD8alpha-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pBS CMV GAG

Plasmid

#234992

Purpose

For production of Viral like particles (VLPs) by expressing the MLV GAG protein in mammalian cells

Depositor

Monica Gonzalez-Magaldi, Daniel Leahy

Article

Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

Insert

GAG

Expression

Mammalian

Mutation

Deleted polymerase coding sequence

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)

Plasmid

#175574

Purpose

Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

Depositor

Joseph Brzezinski

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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CLYBL-TO-PAX7

Plasmid

#204346

Purpose

Knock-in vector to insert a dox-inducible PAX7 transgene into the CYBL locus in human pluripotent stem cells. PAX7 induction results in myogenic differentiation.

Depositor

Ivo Lieberam

Article

Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

Insert

human PAX7

Expression

Mammalian

Promoter

TRE3G

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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mCh-Rab5

Plasmid

#49201

Purpose

expression of Rab5 fused to mCherry

Depositor

Gia Voeltz

Article

Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

Insert

Rab5 (RAB5B Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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mcherry-DN KASH

Plasmid

#125553

Purpose

mcherry labeled DN-KASH

Depositor

Daniel Conway

Article

Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

Insert

SYNE1 (SYNE1 Human)

Tags

mcherry

Expression

Mammalian

Mutation

Truncated form: only the KASH domain of nesprin-…

Promoter

CMV

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pICH47742::p35S-ePEmax3::tEURb7

Plasmid

#227692

Purpose

Golden Gate level 1, position 2, ePEmax3 expression cassette.

Depositor

Jae-Yean Kim

Article

Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

Insert

ePEmax3 being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).

Use

CRISPR

Expression

Plant

Promoter

CaMV 35S

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_FASN

Plasmid

#107138

Purpose

His tagged human FASN cloned in pcDNA3.1 vector for expression in mammalian cells

Depositor

Brijesh Kumar Singh

Insert

Fatty acid synthase (FASN Human)

Tags

His

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-OGT_opt

Plasmid

#190858

Purpose

For mammalian expression of wild-type full-length human ncOGT. The ncOGT is not epitope-tagged, but is codon-optimized for human cells.

Depositor

Gerald Hart

Article

Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

Insert

O-GlcNAc Transferase (OGT Human)

Expression

Mammalian

Mutation

Codon optimized for expression in human cells.

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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SlugCas9-HF

Plasmid

#163796

Purpose

Expresses highly specific SlugCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Mutation

SlugCas9 (R247A, N415A, T421A, R656A)

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3-GFP

Plasmid

#74165

Purpose

Mammalian expression of EGFP

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pLVET-HA-K-RasG12V-IRES-GFP

Plasmid

#107140

Purpose

A bicistronic lentiviral mammalian expression vector for HA-KRasG12V(4B) and GFP

Depositor

Aki Manninen

Article

Zhang et al Oncogene. 2017 Oct 12;36(41):5681-5694.

Insert

HA-tagged Kras4B(G12V) and GFP (KRAS Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hEF-1alpha

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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Zika NS5 RdRp

Plasmid

#204788

Purpose

Bacterial expression of Zika NS5 RdRp

Depositor

ASAP Discovery, Frank von Delft

Insert

Zika NS5 RdRp domain

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pMRX-IP-GFP-LC3-RFP

Plasmid

#84573

Purpose

Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Retroviral

Tags

EGFP and mRFP1

Expression

Mammalian

Available Since

Jan. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-pTH-iCre-WPREpA

Plasmid

#213130

Purpose

Truncated rat TH promoter expressing iCre

Depositor

Ulrik Gether

Article

Lycas et al Cell Rep. 2022 Sep 27;40(13):111431. doi

Insert

iCre

Use

AAV and Mouse Targeting

Tags

None

Expression

Mammalian

Promoter

Truncated TH promoter from rat (Rattus norvegicus…

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-C1

Plasmid

#60560

Purpose

Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis T5 Exonuclease

Plasmid

#206209

Purpose

Inducible expression of T5 exonuclease

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2018 Aug 15;13(8):e0201681. do

Insert

T5 exonuclease

Expression

Bacterial

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pUC57-mitoLbNOX

Plasmid

#74448

Purpose

This plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

Depositor

Vamsi Mootha

Article

Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

Insert

mitoLbNOX

Tags

Flag tag with linker and mitochondrial targeting …

Mutation

Human codon optimized

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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