We narrowed to 17,810 results for: SHE

1. pCMV_eA3A-nCas9

   Plasmid

   #163535

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   eA3A-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLHCX-Flag-mPKM2(C358S)

   Plasmid

   #42513

   Depositor

   Dimitrios Anastasiou

   Article

   Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

   Insert

   Pyruvate Kinase M2 Cys358->Ser
   

   Use

   Retroviral

   Tags

   Flag

   Mutation

   Changed Cysteine 358 to Serine

   Promoter

   CMV

   Available Since

   March 19, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pPV-H1-ccdB-mEF1α-RiH

   Plasmid

   #100598

   Purpose

   piggyBac vector for sgRNA cloning

   Depositor

   Akitsu Hotta

   Article

   Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

   Type

   Empty backbone

   Use

   Piggybac vector

   Expression

   Mammalian

   Promoter

   Human H1 PolIII promoter, human FTH1 promoter, mo…

   Available Since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV_Actb NHEJ donor

   Plasmid

   #97316

   Purpose

   NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb NHEJ donor
   

   Use

   AAV and Mouse Targeting

   Expression

   Mammalian

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5-Flag CASTOR1 I280A

   Plasmid

   #84495

   Purpose

   overexpression of CASTOR1 I280A mutant

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

   Insert

   CASTOR1 I280A (GATSL3 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   I280A

   Promoter

   CMV

   Available Since

   Oct. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pLenti-CaMKIIa-eArch 3.0-EYFP

   Plasmid

   #35514

   Purpose

   Lentiviral expression of CaMKIIa-driven Arch 3.0 for optical inhibition.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   eArch3.0-EYFP
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Enhanced trafficking signal and ER export signal …

   Promoter

   CaMKIIa

   Available Since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. LP455

   Plasmid

   #90198

   Purpose

   pFLAG-CMV2 with cDNA encoding HsNPHP4 aa residues 649-1426

   Depositor

   Lotte Pedersen

   Article

   Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

   Insert

   NPHP4 (NPHP4 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   aa residues 649-1426

   Available Since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV-human CSFI-R-L301S-IRES-GFP

   Plasmid

   #133744

   Purpose

   retroviral expression of CSF1-R mutant

   Depositor

   Martine Roussel

   Article

   Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

   Insert

   CSF1-R (CSF1R Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   L301S

   Promoter

   MSCV

   Available Since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV_Actb MMEJ donor

   Plasmid

   #97315

   Purpose

   MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb MMEJ donor
   

   Use

   AAV and Mouse Targeting

   Expression

   Mammalian

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. CDK4-luciferase full length (2135)

    Plasmid

    #86656

    Purpose

    Luciferase reporter containing CDK4 promoter

    Depositor

    Jaime Modiano

    Article

    Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

    Insert

    CDK4 promoter (CDK4 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    2120 base pairs including up to coding nucleotide…

    Promoter

    CDK4

    Available Since

    June 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. LP453

    Plasmid

    #90184

    Purpose

    pFLAG-CMV2 with cDNA encoding HsNPHP4 aa residues 1-839

    Depositor

    Lotte Pedersen

    Article

    Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

    Insert

    NPHP4 (NPHP4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    aa residues 1-839

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKT-mIDH1(R132H)-Myc-FLAG-IRES-Kat

    Plasmid

    #133779

    Purpose

    Expresses IDH1 with a R132H mutation and has Myc and FLAG tags. IDH1(R132H) also has a katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Insert

    Isocitrate dehydrogenase 1 (IDH1 Human)
    

    Tags

    Myc and FLAG

    Expression

    Mammalian

    Mutation

    Arginine is mutated to histidine at amino acid po…

    Available Since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTrc99S-ssDsbA-EPA-DNNNS-DQNRT

    Plasmid

    #128404

    Purpose

    Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Exotoxin A (toxA P. aeruginosa)
    

    Tags

    6xHis tag and DsbA signal sequence for periplasmi…

    Expression

    Bacterial

    Mutation

    242DNNNS246 and 384DQNRT388 engineered glycosylat…

    Promoter

    trc

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRC11-U6-DR-crRNA-BsmbI(x2); EFS-Puro-WPRE

    Plasmid

    #123360

    Purpose

    Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat, and constitutive expression of puromycin resistance.

    Depositor

    Sidi Chen

    Article

    Chow et al Nat Methods. 2019 May;16(5):405-408. doi

    Insert

    Empty LbCpf1 crRNA cloning cassette
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. GA-DEVD-GB-NES

    Plasmid

    #61020

    Purpose

    green ddFP based caspase-3 biosensor

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP A and ddGFP B
    

    Tags

    HindIII and NES sequence LALKLAGLDIGS placed afte…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

16. mCardinal-Paxillin-22

    Plasmid

    #56171

    Purpose

    Localization: Focal Adhesions, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    Paxillin
    

    Tags

    mCardinal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pUASp YFP Ras85D WT

    Plasmid

    #52242

    Purpose

    Drosophila expression of YFP-Ras85D

    Depositor

    Matthew Scott

    Insert

    Ras85D (Ras85D Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Promoter

    GAL4

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pSR357.10

    Plasmid

    #125106

    Purpose

    Expression of narL(1-131aa)-ydfI(126-213aa) chimera under PLtetO-1, output Promoter PydfJ115

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    narL(REC)-ydfI(DBD)131
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBV-luc TOP2A 1kb promoter

    Plasmid

    #138274

    Purpose

    Luciferase reporter plasmid driven by TOP2A promoter

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    TOP2A promoter sequence
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTrc99S-ssDsbA-TTc-4xDQNAT

    Plasmid

    #128401

    Purpose

    Periplasmic expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Tetanus toxin fragment C domain
    

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pTrc99S-ssDsbA-TTlight-4xDQNAT

    Plasmid

    #128402

    Purpose

    Periplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Tetanus toxin light chain domain
    

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Mutation

    Inactivating E234A mutation

    Promoter

    trc

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pRK5 CASTOR2-Flag Cterm

    Plasmid

    #84490

    Purpose

    overexpression of CASTOR2 with Flag tag

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

    Insert

    CASTOR2 (GATSL2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

23. pET151-Vps4(101-437)-Hcp1

    Plasmid

    #87737

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100

    Promoter

    T7

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. pET28a-His-hPKM2

    Plasmid

    #42515

    Depositor

    Dimitrios Anastasiou

    Article

    Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

    Insert

    Pyruvate Kinase M2 (PKM Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

25. pGEMHE-Pom121-mScarlet

    Plasmid

    #171491

    Purpose

    T7 promotor drives in vitro transcription of mScarlet-tagged mouse Pom121 mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    Pom121 (Pom121 Mouse)
    

    Use

    Pgehme

    Tags

    mScarlet

    Promoter

    T7

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pLenti-CaMKIIa-Mac 3.0-EYFP

    Plasmid

    #35515

    Purpose

    Lentiviral expression of CaMKIIa-driven Mac 3.0 for optical inhibition.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    eMac 3.0-EYFP
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Trafficking signal, ER export

    Promoter

    CaMKIIa

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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27. pCMV_Anc689-nCas9

    Plasmid

    #163526

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    Anc689-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Available Since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pVRC8400-SARS-CoV-2-S2P-BA.4/5-AVI

    Plasmid

    #192112

    Purpose

    Expresses SARS-CoV-2 S2P protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Insert

    SARS-CoV-2-S2P-BA.4/5 (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    T19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, …

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mCardinal-PMP-N-10

    Plasmid

    #56173

    Purpose

    Localization: Peroxisomes, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    PMP (PXMP2 Human)
    

    Tags

    mCardinal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

30. pCDNA3-MARS-p85(iSH2)

    Plasmid

    #205236

    Purpose

    Expresses PLEKAH5 aa 143-271 (K163A and R164A) fused to the iSH2 domain of mouse p85beta

    Depositor

    Jeremy Baskin

    Inserts

    PLEKHA5(143-271)-K163A/R164A (PLEKHA5 Human)
    p85(iSH2) (Pik3r1 Mouse)
    

    Tags

    Nuclear Export Sequence and mCherry

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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