We narrowed to 11,820 results for: cel.2

1. pQE60-mPU.1-ETS

   Plasmid

   #85733

   Purpose

   Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272, in M15 cells already pre-transformed with 2nd plasmid, pREP-4

   Depositor

   Gregory Poon

   Article

   Poon et al J Biol Chem. 2012 May 25;287(22):18297-3

   Insert

   ETS domain of transcription factor PU.1 (Spi1 Mouse)
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-MtPylRS (human-opti)

   Plasmid

   #164080

   Purpose

   To express a Methanosarcina thermophila derived PylRS in mammalian cells for genetic code expansion

   Depositor

   Tao Liu

   Article

   Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

   Insert

   MtPylRS-478(human-opti)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-MfBulKRS (human-opti)

   Plasmid

   #164196

   Purpose

   To express a Methanosarcina flavescens derived BulKRS in mammalian cells for genetic code expansion

   Depositor

   Tao Liu

   Article

   Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

   Insert

   Mf BulKRS(human-opti)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Y269A;Y347F

   Promoter

   CMV

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Ro2

   Plasmid

   #24503

   Depositor

   Bruce Conklin

   Article

   Coward et al Proc Natl Acad Sci U S A. 1998 Jan 6;95(

   Insert

   RASSL Ro2 (OPRK1 Human)
   

   Use

   Tags

   FLAG and NLS from prolactin

   Expression

   Mammalian

   Mutation

   mutations in extracellular loops 2 (see comments …

   Promoter

   Available since

   June 16, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pJB180

   Plasmid

   #235737

   Purpose

   Expression of mCherry- human RAB1A Q70L in mammalian cells

   Depositor

   Roger Williams

   Article

   Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

   Insert

   RAB1A (RAB1A Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Q70L

   Promoter

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. Pum NaVB2

   Plasmid

   #200231

   Purpose

   Expression in mammalian cells or oocytes

   Depositor

   Dan Minor

   Article

   Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

   Insert

   Pitohui uropygialis meridionalis NaV beta 2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. NMUR2-DuET

   Plasmid

   #213350

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   NMUR2 (NMUR2 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. SSTR1-DuET

   Plasmid

   #213376

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   SSTR1 (SSTR1 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTV-Flnc-5prime-1000-24MS96T-3F_NeoR

   Plasmid

   #174881

   Purpose

   Targeting vector for generating Flnc STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   STREAMING-tag with homology arms for Flnc
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTV-Usp5-5prime-1000-24MS96T-3F_NeoR

    Plasmid

    #174885

    Purpose

    Targeting vector for generating Usp5 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    STREAMING-tag with homology arms for Usp5
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTV-Wnk1-5prime-1000-24MS96T-3F_NeoR

    Plasmid

    #174886

    Purpose

    Targeting vector for generating Wnk1 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    STREAMING-tag with homology arms for Wnk1
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAc-His-zfCsf1b

    Plasmid

    #168104

    Purpose

    Express soluble His-tagged zebrafish Csf1b in insect cells

    Depositor

    Petr Bartunek

    Article

    Hason et al Blood Adv. 2022 Jan 3. pii: 483305. doi:

    Insert

    Csf1b (csf1b Zebrafish)
    

    Use

    Tags

    6x His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Halo-WIPI2B H85E K88E

    Plasmid

    #175032

    Purpose

    Expresses WIPI2B (H85E K88E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

    Depositor

    Andrea Stavoe

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2B (H85E K88E) (WIPI2 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Histadine 85 to Glutamate (CAC to GAG); Lysine 88…

    Promoter

    CMV

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF-DEST51 PNRC1

    Plasmid

    #123293

    Purpose

    Expression of PNRC1 in mammalian cells

    Depositor

    Giovanni Tonon

    Article

    Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

    Insert

    PNRC1 (PNRC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFUSE-rIgG-Fc2-ICAM1

    Plasmid

    #156462

    Purpose

    Expresses a rabbit Fc-ICAM1 fusion protein in mammalian cells

    Depositor

    Dirk Mielenz

    Article

    Reimer et al Cell Rep. 2020 Aug 11;32(6):108030. doi:

    Insert

    Intercellular Adhesion Molecule 1 (Icam1 Mouse)
    

    Use

    Tags

    ICAM1-rFc

    Expression

    Bacterial and Mammalian

    Mutation

    endogenous signal peptide replaced with IL2 signa…

    Promoter

    hEF1-HTLC

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-NUP188

    Plasmid

    #159893

    Purpose

    Expresses NUP188 in mammalian cells

    Depositor

    Kozo Tanaka

    Article

    Itoh et al Cancer Sci. 2013 Jul;104(7):871-9. doi:

    Insert

    NUP188 (NUP188 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    To avoid NotI site, first three amino acids are d…

    Promoter

    CMV

    Available since

    Oct. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-CMV-NFE2L2-Puro

    Plasmid

    #181919

    Purpose

    Lentiviral plasmid that directs the expression of NFE2L2/Nrf2 in mammalian cells.

    Depositor

    Scott Dixon

    Article

    Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

    Insert

    NFE2L2 (NFE2L2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAX-EGFP-FBP17

    Plasmid

    #178365

    Purpose

    Expresses FBP17 c-terminal labeled with EGFP in mammalian cells

    Depositor

    Erik Dent

    Article

    Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

    Insert

    Formin Binding Protein 17 (FNBP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta Actin

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMJA285= pSin-TCRab-CMVp-Puro

    Plasmid

    #116875

    Purpose

    stable TCR expression in human T cells

    Depositor

    Marcos Alcocer

    Article

    Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

    Insert

    TCR (TRAV10, TRBV25-1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    deletion of EF1-a promotor

    Promoter

    CMV Promotor

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FP5369 KIF17MD-flag- FIREmate

    Plasmid

    #216724

    Purpose

    Expresses motor domain of KIF17 fused to FIREmate in mamallian cells

    Depositor

    Franck Perez

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    KIF17 (KIF17 Human)
    

    Use

    Tags

    FIREmate and Flag

    Expression

    Mammalian

    Mutation

    1-547

    Promoter

    CMV

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CMV-10-3xFLAG-LGP2

    Plasmid

    #58681

    Purpose

    Expresses 3x Flag-tagged LGP2 in mammalian cells

    Depositor

    Curt Horvath

    Article

    Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

    Insert

    laboratory of genetics and physiology 2 (DHX58 Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TMEM16F-I521K-peGFP-N1

    Plasmid

    #228588

    Purpose

    Expresses TMEM16F-I521K-peGFP in mammalian cells

    Depositor

    Huanghe Yang

    Article

    Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

    Insert

    TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    I521K plus a 3 amino acid N-terminal truncation (…

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TMEM16F-I611K-peGFP-N1

    Plasmid

    #228581

    Purpose

    Expresses mouse TMEM16F-I611K-eGFP in mammalian cells

    Depositor

    Huanghe Yang

    Article

    Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

    Insert

    TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    I611K plus a 3 amino acid N-terminal truncation (…

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3-FLAG-HA-CAD

    Plasmid

    #46238

    Purpose

    For expression of HA and FLAG-tagged murine CAD in mammalian cells

    Depositor

    Brendan Manning

    Article

    Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

    Insert

    carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MoFGFR3b-SV40PA

    Plasmid

    #86493

    Purpose

    mamalian cell expression of FGFR3b

    Depositor

    David Ornitz

    Article

    Chellaiah et al J Biol Chem. 1994 Apr 15;269(15):11620-7

    Insert

    FgfrR3 IIIb 12F5 (Fgfr3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Moloney murine leukemia virus LTR

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-PE2-tagRFP-BleoR

    Plasmid

    #192508

    Purpose

    Expression of PE2 in mammalian cells with co-expression of tagRFP and Zeocin resistance gene.

    Depositor

    Gerald Schwank

    Article

    Mathis et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    PE2-P2A-tagRFP-P2A-BleoR
    

    Use

    Tags

    BleoR, SV40 bpNLS, and tagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSF3-Flag-Cbir-Ago2_Myc-Nbir-Dicer

    Plasmid

    #90013

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-Dicer

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    CBirA*-Ago2
    NBirA*-Dicer
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEGFP-C1-IFT43

    Plasmid

    #218735

    Purpose

    Expresses N-terminally EGFP-tagged IFT43 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

    Insert

    IFT43 (IFT43 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEF-Bos-FLAG MDA5 MI

    Plasmid

    #52875

    Purpose

    Expresses MDA5 with a mutation to motif I in mammalian cells

    Depositor

    Curt Horvath

    Article

    Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

    Insert

    IFIH1 (IFIH1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K335A, E658K

    Promoter

    Available since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSF3-Flag-Cbir-Ago2_Myc-Nbir-GFP

    Plasmid

    #90015

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-GFP

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    CBirA*-Ago2
    NBirA*-GFP
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge