We narrowed to 9,707 results for: crispr plasmids

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AAVS1-iPE2-Hygro

Plasmid

#214019

Purpose

inducible PE2 system for controllable prime editing; this plasmid is used to insert PE2 prime editor in one allele of AAVS1 locus

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

PE2

Expression

Mammalian

Promoter

TRE-tight

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-SA-WTmLdlrEx14-gRNA2-N22-HLP-SACas9-HA-OLLAS-spA

Plasmid

#206860

Purpose

An AAV plasmid with U6 promoter driving a gRNA against LDLR and liver specific HLP promoter driving SaCas9

Depositor

William Lagor

Article

De Giorgi et al Methods Mol Biol. 2022;2419:673-713. doi

Insert

mLdlr gRNA (Ldlr Mouse)

Use

AAV and CRISPR

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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FUGW U6 gL1HSg1dCas9-KRAB-T2a-GFP

Plasmid

#234882

Purpose

L1HS-silencing plasmid (CRISPRi gRNA1)

Depositor

Johan Jakobsson

Article

Adami et al bioRxiv 2025.01.17.633315

Insert

L1HS-silencing plasmid (CRISPRi gRNA1)

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-ABE-NT-sgRNA

Plasmid

#112734

Purpose

AAV inverted terminal repeat based vector plasmid encoding E. coli TadA, the N-terminal half of nCas9 and sgRNA

Depositor

Jin-Soo Kim

Article

Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

Insert

ABE-NT-sgRNA

Use

AAV

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pL90-Nat-2xHO

Plasmid

#139069

Purpose

CRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selection

Depositor

Gianni Liti

Article

Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

Insert

Two guide RNAs targeting the HO endonuclease

Expression

Bacterial and Yeast

Promoter

TDH3

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1

Plasmid

#121824

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Sa-dCas9/LSD1 (open)

Use

Synthetic Biology; Pmagic gateway entry plasmid

Tags

NLS

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSLiP-G2-RacE

Plasmid

#188966

Purpose

L-Rhamnose inducible catalytically inactive Cas9 with sgRNA and racE gene for bacterial gene knockdown

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

dCas9
sgRNA: gttagacgctgattacatggactagg
racE

Use

Synthetic Biology

Expression

Bacterial

Promoter

PrhaBAD

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.U6gLacZ.hSyn.H2B.RFP

Plasmid

#170369

Purpose

Negative control plasmid used for Crispr/cas9 based disruption. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B RFP

Use

AAV

Promoter

human Synapsin

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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KL301: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-p300core

Plasmid

#121839

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS/p300core scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Synthetic Biology; Pmagic gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LPUtopia-7_CoV-2

Plasmid

#224782

Purpose

Landing Pad donor vector containing partial RdRp and N gene sequences from SARS-CoV-2 for fluorescent reporter assay

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Insert

partial RdRp and N gene (N, ORF1ab SARS-CoV-2)

Expression

Mammalian

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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MTOR-F2108L_DARIC(1)_CD19-scFv_AAV6_Donor

Plasmid

#211905

Purpose

Vector for AAV6 donor production. Targeted CD19-scFv DARIC transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.

Depositor

Yannick Doyon

Article

Levesque et al bioRxiv 2023.09.14.557485

Insert

DARIC-CD19-scFv

Use

AAV

Expression

Mammalian

Promoter

hPGK1

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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hsLEMD2-pX330

Plasmid

#179511

Purpose

Encodes gRNA for human LEMD2

Depositor

Yasushi Hiraoka

Article

Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

Insert

gRNA for LEMD2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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Cas9_nonPuro_OSS

Plasmid

#186652

Purpose

Cas9 plasmid to express the Cas9 in OSS cells

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS Cas9 Expression Plasmid

Expression

Insect

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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IF405: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-p300core

Plasmid

#121825

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the p300 catalytic core for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Sa-dCas9/p300core (open)

Use

Synthetic Biology; Pmagic gateway entry plasmid

Tags

NLS

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KL001: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-VPR

Plasmid

#121836

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS/VPR scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Synthetic Biology; Pmagic gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KL202: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-LSD1

Plasmid

#121838

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS/LSD1 scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Synthetic Biology; Pmagic gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IA304: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-VPR

Plasmid

#121822

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the VPR transcriptional activator for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Sa-dCas9/VPR (open)

Use

Synthetic Biology; Pmagic gateway entry plasmid

Tags

NLS

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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Ring2-FLAG-AID-mClover

Plasmid

#246226

Purpose

Expression of Ring2-FLAG-AID

Depositor

Hengbin Wang

Insert

Ring2 (RNF2 Human)

Use

CRISPR

Tags

Flag-AID-mClover

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-puro-hGSDMD

Plasmid

#185377

Purpose

For mammalian expression of guide RNA: CGCGCCAGACGCGCCACCCT that targets human GSDMD (Gasdermin D)

Depositor

Ayaz Najafov

Insert

GSDMD (GSDMD Human)

Expression

Mammalian

Mutation

WT

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9_mouse_Adar1_ccB

Plasmid

#158122

Purpose

deletion mAdar1

Depositor

Emanuela Felley-Bosco

Insert

Adar1 (Adar Mouse)

Expression

Mammalian

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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