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pJBL717

Plasmid

#140387

Purpose

Expresses MobR protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-MobR-T

Tags

TEV-6XHis

Expression

Bacterial

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pMGE-T7

Plasmid

#153021

Purpose

Single-plasmid multi-gene expression system. Each genes under the control of a T7 and the lacO promoter sequences.

Depositor

Vito Valiante

Article

Kufs et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pGT-B3

Plasmid

#122581

Purpose

MAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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B7-1

Plasmid

#111489

Purpose

translational elongation factor G

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational elongation factor G

Tags

6xHis

Expression

Bacterial

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_S-Luc_CLIP

Plasmid

#113923

Purpose

Plasmid encoding the bacterial expression of the chimeric SNAP-NanoLuc fusion dubbed S-Luc c-terminally linked to CLIP-tag for BRET

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

S-Luc_CLIP

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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B6-1

Plasmid

#111485

Purpose

Phe-tRNA synthetase B

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Phe-tRNA synthetase B

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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B3-1

Plasmid

#111471

Purpose

translational initiation factor 2

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational initiation factor 2

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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B4-5

Plasmid

#111479

Purpose

ribosome recycling factor

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

ribosome recycling factor

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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B3-3

Plasmid

#111473

Purpose

Glu-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Glu-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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B6-2

Plasmid

#111486

Purpose

Pro-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Pro-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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B7-3

Plasmid

#111491

Purpose

Tyr-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Tyr-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pPD0029_pIVTRup(BsmBI)_SpCas9

Plasmid

#242535

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

SpCas9

Use

CRISPR

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pEND-320_Cacnes_ori

Plasmid

#225602

Purpose

Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a low-copy pBR322 E. coli ori

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pKJ0463

Plasmid

#228715

Purpose

Bacterial expression of epT7 RNAP; for protein production and purification

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Science 0, eadr6006

Insert

Sumo_epT7

Expression

Bacterial

Mutation

T3M/G47A/E643G

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19/Vc-C

Plasmid

#183158

Purpose

Bimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vc.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_Puro

Plasmid

#167863

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_Blasto

Plasmid

#167856

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S

Plasmid

#149638

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, empty

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK527

Plasmid

#110605

Purpose

BTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with kanamycin resistance)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pJMP1223

Plasmid

#119261

Purpose

rfp "test" strain

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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