We narrowed to 13,072 results for: BASE;
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Plasmid#89578PurposecaCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locusDepositorInsertLeu2 gene drive
ExpressionYeastAvailable SinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIRESpuro-Flag-Pol b
Plasmid#23257DepositorAvailable SinceMay 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1_3xFLAG-TAOK1
Plasmid#172991Purposeconstitutive expression of FLAG-tagged TAOK1DepositorAvailable SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HLA-cMyc-EcopT1R1
Plasmid#113962Purposemammalian expression plasmid for c-myc-tagged E. coli codon-optimized human T1R1 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chainDepositorInsertT1R1 (TAS1R1 Human)
TagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationtruncate N-terminal 24 residuesAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-TorA(sp)-mNeonGreen
Plasmid#205020PurposeBroad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorA signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasmDepositorInsertTorA-mNeonGreen
ExpressionBacterialMutationmNeonGreen is codon optimized for expression in P…PromoterPcAvailable SinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
TC1316
Plasmid#164883PurposeCMV-bpNLS-dCas13d-bpNLS-ADARdd-mCherry, ADARdd embeded in loop3DepositorInsertdcas13d-ADARdd
ExpressionMammalianMutationdCas13d L560-G586 deletedPromoterCMV, T7Available SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQ54-VC
Plasmid#175060Purposeexpresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ54) in mammalian cellsDepositorInsertpolyQ54 (HTT Human)
TagsmVenus + mCherryExpressionMammalianMutationmVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…PromoterCMV PromoterAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQ25-VC
Plasmid#175062Purposeexpresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ25) in mammalian cellsDepositorInsertpolyQ25 (HTT Human)
TagsmVenus + mCherryExpressionMammalianMutationmVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…PromoterCMV PromoterAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Blast-mNeon-ACTB
Plasmid#207751PurposeDonor template for Blast-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748DepositorInsertACTB Homology Arms flanking a Blast-mNeon Cassette (ACTB Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
YET1000: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X4)
Plasmid#104571PurposeMammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to four DmrA domainsDepositorInserthuman codon optimized ‘dead’ Cpf1 fused to four DmrA domains
UseCRISPRTagsNLS-3xHA-3xFLAG-4xDmrAExpressionMammalianMutationD832APromoterCAGAvailable SinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-2
Plasmid#228984PurposeFor bacterial expression of anti-GST nanobody GST-2, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-2
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG94-1--LaG94-14
Plasmid#228973PurposeFor bacterial expression of anti-GFP nanobody dimer of LaG94-1 and LaG94-14, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertLaG94-1--LaG94-14
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-1
Plasmid#228983PurposeFor bacterial expression of anti-GST nanobody GST-1, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-1
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaTdT-1
Plasmid#228974PurposeFor bacterial expression of anti-tdTomato nanobody LaTdT-1, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.DepositorInsertLaTdT-1
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaRIgG-7
Plasmid#228997PurposeFor bacterial expression of anti-rabbit IgG nanobody LaRIgG-7, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaRIgG-7
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaRIgG-1
Plasmid#228995PurposeFor bacterial expression of anti-rabbit IgG nanobody LaRIgG-1, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaRIgG-1
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaMIgG-5
Plasmid#228991PurposeFor bacterial expression of anti-mouse IgG nanobody LaMIgG-5, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaMIgG-5
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
CD3-2A_pMI-LO
Plasmid#153418PurposeRetroviral (MSCV) expression of all four WT CD3 subunits, co-expressed with LSSmOrangeDepositorInsertmurine CD3 delta-gamma-epsilon-zeta subunits (Cd3d Mouse)
UseRetroviralExpressionMammalianAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEL066
Plasmid#124897PurposeFor genome editing in plant by SpCas9 variant(SpCas9-NG) (D10A)-PmCDA1-UGI base editorDepositorTypeEmpty backboneUseCRISPRTags3xFlagExpressionPlantAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA GFP10-RHOA
Plasmid#133068PurposeExpresses RHOA GTPase with tripartite split GFP10DepositorInsertras homolog family member A (RHOA Human)
TagsGFP10 (split-GFP tripartite)ExpressionMammalianPromoterCMVAvailable SinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only