2,223 results

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pminiTol2-4kb wnt3 promoter-Wnt3EGFP

Plasmid

#203793

Purpose

Tol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of Wnt3-EGFP for zebrafish transgenesis

Depositor

Thorsten Wohland

Article

Teh et al Development. 2015 Nov 1;142(21):3721-33.

Insert

Wnt3 (wnt3 Zebrafish)

Use

Zebrafish expression

Tags

mEGFP

Promoter

4kb Wnt3 promoter

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pminiTol2-4kb Wnt3 promoter-EGFP

Plasmid

#203792

Purpose

Tol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of EGFP for zebrafish transgenesis

Depositor

Thorsten Wohland

Article

Teh et al Development. 2015 Nov 1;142(21):3721-33.

Insert

EGFP

Use

Zebrafish expression

Promoter

4kb Wnt3 promoter

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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acvr1l-HA

Plasmid

#201904

Purpose

in vitro transcription of RNA encoding HA-tagged Acvr1l

Depositor

Mary Mullins

Article

Tajer et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

acvr1l (acvr1l Zebrafish)

Tags

HA

Promoter

SP6

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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p5E_(-3kb)nrg1 type II

Plasmid

#199393

Purpose

pDEL61; Gateway 5' Entry clone encoding nrg1 type II upstream regulatory sequence

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

(-3kb)nrg1 type II

Use

Gateway Cloning

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2_claudink:Cas9green; erbb2_gRNA171

Plasmid

#199339

Purpose

pDEL142; transgenic construct to express cell-specific Cas9 in oligodendrocytes; ubiquitous erbb2 sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

claudink
Cas9
erbb2 sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2_(-3kb)nrg1_typeII:GFP-CAAX

Plasmid

#199394

Purpose

pDEL63; transgenic construct to express GFP-CAAX under nrg1 type II regulatory region control

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

(-3kb)nrg1 type II
GFP-CAAX
afp_3'_UTR

Use

Tol2 gateway destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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zCREST3:Cas9green; T3_gRNA

Plasmid

#199335

Purpose

pDEL161; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type III sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
nrg1 type III sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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zCREST3:Cas9green; EGF_gRNA

Plasmid

#199333

Purpose

pDEL158; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 EGF sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
nrg1 EGF sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCK074 p5E exorh:EGFP

Plasmid

#195951

Purpose

Gateway p5E vector with reporter

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

exorh:EGFP

Use

Other

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCK072 p5E 147bp exorh:EGFP

Plasmid

#195950

Purpose

Gateway p5E vector with reporter

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

exorh minimal regulatory elements and EGFP

Use

Other

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-GFP-U6ac-rac2-guides

Plasmid

#168241

Purpose

"neutrophil specific GFP with ubiquitous rac2 sgRNAs"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

rac2 sgRNAs

Use

CRISPR

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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mKate2-DrCavin1b in pT3TS-Dest

Plasmid

#194295

Purpose

In vitro transcription of mKate2 tagged zebrafish cavin1b from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAC

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

cavin1b (cavin1b Zebrafish)

Use

In vitro transcription of mrna

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST_6xUAS:miRFP670-cetn4

Plasmid

#192357

Purpose

zfCETN4 tagged with miRFP670, expressed under the UAS promoter

Depositor

Darren Gilmour

Article

Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

Insert

CETN4 (cetn4 Zebrafish)

Tags

miRFP670

Expression

Mammalian

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pBK-CMV-mpx

Plasmid

#191826

Purpose

mpx probe generation for in situ

Depositor

Jason Berman

Article

Biggs et al JCI Insight. 2022 Dec 22;7(24):e150849.

Insert

mpx cDNA (mpx Zebrafish)

Expression

Mammalian

Promoter

CMV promoter

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAc-His-zfCsf1a

Plasmid

#168103

Purpose

Express soluble His-tagged zebrafish Csf1a in insect cells

Depositor

Petr Bartunek

Article

Hason et al Blood Adv. 2022 Jan 3. pii: 483305. doi:

Insert

Csf1a (csf1a Zebrafish)

Tags

6x His

Expression

Insect

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-zfIl34

Plasmid

#168112

Purpose

Express zebrafish Il34, mRNA synthesis

Depositor

Petr Bartunek

Article

Hason et al Blood Adv. 2022 Jan 3. pii: 483305. doi:

Insert

Il34 (il34 Zebrafish)

Use

Vertebrate expression (zebrafish, chicken, xenopu…

Expression

Mammalian

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-mCherry-6H

Plasmid

#187104

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Tags

mCherry

Expression

Bacterial

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-Y90F-V91F-EGFP-6H

Plasmid

#187186

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Bacterial

Mutation

Y90F, V91F

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2z-tal1

Plasmid

#164656

Purpose

for the synthesis of zebrafish tal1 mRNA

Depositor

Didier Stainier

Article

Mattonet et al Sci Adv. 2022 Sep 2;8(35):eabn2082. doi:

Insert

tal1 (tal1 Zebrafish)

Use

Mrna synthesis

Promoter

SP6

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pTOPO-col10a1-KI-donor

Plasmid

#184874

Purpose

Donor template for knock-in of p2a-CreERT2 into the medaka col10a1 locus via CRISPR/Cas9-mediated homology directed repair

Depositor

Christoph Winkler

Article

Hui et al Development. 2022 May 20. pii: 275483. d

Inserts

Collagen10a1 5' homology arm
Collagen10a1 3' homology arm
p2a-CreERT2
Myosin light chain 2 promoter
eGFP

Use

CRISPR and Cre/Lox

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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