2,246 results

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pminiTol2-4kb Wnt3 promoter-EGFP

Plasmid

#203792

Purpose

Tol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of EGFP for zebrafish transgenesis

Depositor

Thorsten Wohland

Publication

Teh et al Development. 2015 Nov 1;142(21):3721-33.

Insert

EGFP

Use

Zebrafish expression

Promoter

4kb Wnt3 promoter

Available since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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acvr1l-HA

Plasmid

#201904

Purpose

in vitro transcription of RNA encoding HA-tagged Acvr1l

Depositor

Mary Mullins

Publication

Tajer et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

acvr1l (acvr1l Zebrafish)

Tags

HA

Promoter

SP6

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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p5E_(-3kb)nrg1 type II

Plasmid

#199393

Purpose

pDEL61; Gateway 5' Entry clone encoding nrg1 type II upstream regulatory sequence

Depositor

William Talbot

Publication

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

(-3kb)nrg1 type II

Use

Gateway Cloning

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2_claudink:Cas9green; erbb2_gRNA171

Plasmid

#199339

Purpose

pDEL142; transgenic construct to express cell-specific Cas9 in oligodendrocytes; ubiquitous erbb2 sgRNA

Depositor

William Talbot

Publication

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

claudink
Cas9
erbb2 sgRNA

Use

Tol2 destination vector

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2_(-3kb)nrg1_typeII:GFP-CAAX

Plasmid

#199394

Purpose

pDEL63; transgenic construct to express GFP-CAAX under nrg1 type II regulatory region control

Depositor

William Talbot

Publication

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

(-3kb)nrg1 type II
GFP-CAAX
afp_3'_UTR

Use

Tol2 gateway destination vector

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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zCREST3:Cas9green; T3_gRNA

Plasmid

#199335

Purpose

pDEL161; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type III sgRNA

Depositor

William Talbot

Publication

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

zCREST3
Cas9
nrg1 type III sgRNA

Use

Tol2 destination vector

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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zCREST3:Cas9green; EGF_gRNA

Plasmid

#199333

Purpose

pDEL158; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 EGF sgRNA

Depositor

William Talbot

Publication

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

zCREST3
Cas9
nrg1 EGF sgRNA

Use

Tol2 destination vector

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCK074 p5E exorh:EGFP

Plasmid

#195951

Purpose

Gateway p5E vector with reporter

Depositor

Christian Mosimann

Publication

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

exorh:EGFP

Use

Other

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCK072 p5E 147bp exorh:EGFP

Plasmid

#195950

Purpose

Gateway p5E vector with reporter

Depositor

Christian Mosimann

Publication

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

exorh minimal regulatory elements and EGFP

Use

Other

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-GFP-U6ac-rac2-guides

Plasmid

#168241

Purpose

"neutrophil specific GFP with ubiquitous rac2 sgRNAs"

Depositor

Qing Deng

Publication

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

rac2 sgRNAs

Use

CRISPR

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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mKate2-DrCavin1b in pT3TS-Dest

Plasmid

#194295

Purpose

In vitro transcription of mKate2 tagged zebrafish cavin1b from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAC

Depositor

Rob Parton

Publication

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

cavin1b (cavin1b Zebrafish)

Use

In vitro transcription of mrna

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST_6xUAS:miRFP670-cetn4

Plasmid

#192357

Purpose

zfCETN4 tagged with miRFP670, expressed under the UAS promoter

Depositor

Darren Gilmour

Publication

Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CETN4 (cetn4 Zebrafish)

Tags

miRFP670

Expression

Mammalian

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pBK-CMV-mpx

Plasmid

#191826

Purpose

mpx probe generation for in situ

Depositor

Jason Berman

Publication

Biggs et al JCI Insight. 2022 Dec 22;7(24):e150849.

Insert

mpx cDNA (mpx Zebrafish)

Expression

Mammalian

Promoter

CMV promoter

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAc-His-zfCsf1a

Plasmid

#168103

Purpose

Express soluble His-tagged zebrafish Csf1a in insect cells

Depositor

Petr Bartunek

Publication

Hason et al Blood Adv. 2022 Jan 3. pii: 483305. doi:

Insert

Csf1a (csf1a Zebrafish)

Tags

6x His

Expression

Insect

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-zfIl34

Plasmid

#168112

Purpose

Express zebrafish Il34, mRNA synthesis

Depositor

Petr Bartunek

Publication

Hason et al Blood Adv. 2022 Jan 3. pii: 483305. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Il34 (il34 Zebrafish)

Use

Vertebrate expression (zebrafish, chicken, xenopu…

Expression

Mammalian

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-mCherry-6H

Plasmid

#187104

Purpose

Protein Expression

Depositor

Philipp Niethammer

Publication

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Tags

mCherry

Expression

Bacterial

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-Y90F-V91F-EGFP-6H

Plasmid

#187186

Purpose

Protein Expression

Depositor

Philipp Niethammer

Publication

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Bacterial

Mutation

Y90F, V91F

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2z-tal1

Plasmid

#164656

Purpose

for the synthesis of zebrafish tal1 mRNA

Depositor

Didier Stainier

Publication

Mattonet et al Sci Adv. 2022 Sep 2;8(35):eabn2082. doi:

Insert

tal1 (tal1 Zebrafish)

Use

Mrna synthesis

Promoter

SP6

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pTOPO-col10a1-KI-donor

Plasmid

#184874

Purpose

Donor template for knock-in of p2a-CreERT2 into the medaka col10a1 locus via CRISPR/Cas9-mediated homology directed repair

Depositor

Christoph Winkler

Publication

Hui et al Development. 2022 May 20. pii: 275483. d

Inserts

Collagen10a1 5' homology arm
Collagen10a1 3' homology arm
p2a-CreERT2
Myosin light chain 2 promoter
eGFP

Use

CRISPR and Cre/Lox

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+ Zf cdh1-deltaCyot-GFP

Plasmid

#185412

Purpose

GFP tagged zebrafish cdh1 lacking the cytoplasmic domain.

Depositor

Nicolas David

Publication

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

cdh1 (cdh1 Zebrafish)

Use

In vitro synthesis of mrna

Tags

eGFP

Expression

Mammalian

Mutation

Cytoplasmic domain deleted

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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