We narrowed to 3,042 results for: NAK

1. pcDNA C12 ecDHFR DD YFP HA

   Plasmid

   #192815

   Purpose

   Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.

   Depositor

   John Hulleman

   Article

   Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021

   Insert

   E. coli dihydrofolate reductase
   

   Tags

   enhanced yellow fluorescent protein and hemagglut…

   Expression

   Mammalian

   Mutation

   W74R/T113S/E120D/Q146L

   Promoter

   CMV

   Available Since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-Foxo1ADA (pCMV5)

   Plasmid

   #12143

   Depositor

   Domenico Accili

   Article

   Nakae et al J Clin Invest. 2001 Nov . 108(9):1359-67

   Insert

   Foxo1 ADA (Foxo1 Mouse)
   

   Tags

   HA and myc

   Expression

   Mammalian

   Mutation

   Phosphorylation-deficient, also referred to as co…

   Promoter

   CMV

   Available Since

   Aug. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-Myc-CEBPA

   Plasmid

   #219393

   Purpose

   Express CEBPA in mammalian cells

   Depositor

   Yoichi Furukawa

   Article

   Nakagawa et al Commun Biol. 2024 Apr 29;7(1):510. doi:

   Insert

   CEBPA (CEBPA Human)
   

   Tags

   N terminal Myc tag

   Expression

   Mammalian

   Available Since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCXLE-hGLIS1

   Plasmid

   #37625

   Purpose

   Non-integrating (episomal) expression of human GLIS1

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

   Insert

   human GLIS1 (GLIS1 Human)
   

   Expression

   Mammalian

   Mutation

   T110A, A187G, I221T

   Promoter

   CAG

   Available Since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. Myc-Foxo1 D256

   Plasmid

   #12145

   Depositor

   Domenico Accili

   Article

   Nakae et al EMBO J. 2000 Mar 1. 19(5):989-96.

   Insert

   Foxo1 (Foxo1 Mouse)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Contains a deletion truncating the protein after …

   Promoter

   CMV

   Available Since

   Aug. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-Myc-FOXA1

   Plasmid

   #219394

   Purpose

   Express FOXA1 in mammalian cells

   Depositor

   Yoichi Furukawa

   Article

   Nakagawa et al Commun Biol. 2024 Apr 29;7(1):510. doi:

   Insert

   FOXA1 (FOXA1 Human)
   

   Tags

   N terminal Myc tag

   Expression

   Mammalian

   Available Since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-NUP188

   Plasmid

   #159893

   Purpose

   Expresses NUP188 in mammalian cells

   Depositor

   Kozo Tanaka

   Article

   Itoh et al Cancer Sci. 2013 Jul;104(7):871-9. doi:

   Insert

   NUP188 (NUP188 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   To avoid NotI site, first three amino acids are d…

   Promoter

   CMV

   Available Since

   Oct. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-EGFP-C-IFT88

   Plasmid

   #218733

   Purpose

   Expresses N-terminally EGFP-tagged IFT88 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Ishida et al Mol Biol Cell. 2022 Aug 1;33(9):ar83. do

   Insert

   IFT88 (IFT88 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. peSpCas9(1.1)-2×sgRNA (empty, donor)

   Plasmid

   #80768

   Purpose

   All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains eSpCas9(1.1) and two sgRNA expression cassettes. The first gRNA cloning site is empty.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. dmBACCS2-IRES-dOrai-IRES-mCherry

    Plasmid

    #72894

    Purpose

    Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2-IRES-dOrai-IRES-mCherry
    

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-Sox2-IRES-Neo

    Plasmid

    #13462

    Depositor

    Shinya Yamanaka

    Article

    Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

    Insert

    Sox2 (Sox2 Mouse)
    

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pG2J

    Plasmid

    #102882

    Purpose

    Expresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.

    Depositor

    Matthias Heinemann

    Article

    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

    Inserts

    OsTIR1
    mGFP-AID
    

    Tags

    AID(71-114)

    Expression

    Yeast

    Promoter

    ADH1 and TEF1

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET22b Ag43 160N 6H sfGFP-R5 AmpR

    Plasmid

    #225139

    Purpose

    Recombinant Ag43 protein fused to silaffin-R5 and sfGFP. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.

    Depositor

    Urartu Seker

    Article

    Kirpat Konak et al Mater Today Bio. 2022 Oct 10;17:100461.

    Insert

    Ag43 protein fused to silaffin-R5 and sfGFP
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-ecDHFR(DD)-MS2CP-VPg(FCV)

    Plasmid

    #167313

    Purpose

    Template pDNA to prepare DD-CaVT mRNA

    Depositor

    Hirohide Saito

    Article

    Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

    Insert

    DD-CaVT
    

    Use

    Synthetic Biology

    Tags

    DYK-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. 1099 pcDNA GFP FKHR AAA

    Plasmid

    #9023

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    FKHR AAA (FOXO1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    T24A S256A S319A

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-CHAMP1

    Plasmid

    #159892

    Purpose

    Expresses CHAMP1 (CAMP) in mammalian cells

    Depositor

    Kozo Tanaka

    Article

    Itoh et al EMBO J. 2011 Jan 5;30(1):130-44. doi: 10

    Insert

    CHAMP1 (CHAMP1 Human)
    

    Tags

    pEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax

    Plasmid

    #173871

    Purpose

    Mammalian expression of mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax (opto-Rac1)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax
    

    Tags

    C-terminal CAAX-box and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-EGFP-C-IFT81

    Plasmid

    #218732

    Purpose

    Expresses N-terminally EGFP-tagged IFT81 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Ishida et al Mol Biol Cell. 2022 Aug 1;33(9):ar83. do

    Insert

    IFT81 (IFT81 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-C1-IFT43

    Plasmid

    #218735

    Purpose

    Expresses N-terminally EGFP-tagged IFT43 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

    Insert

    IFT43 (IFT43 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNAz-mBACEflag (C474A/C478A/C482A/C485A)

    Plasmid

    #26736

    Depositor

    Gopal Thinakaran

    Article

    Vetrivel et al J Biol Chem. 2009 Feb 6. 284(6):3793-803

    Insert

    BACE (Bace1 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Cys 474, 478, 482, and 485 mutated to Ala

    Available Since

    Dec. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

21. pCAG-EGFP-C-IFT74

    Plasmid

    #218730

    Purpose

    Expresses N-terminally EGFP-tagged IFT74 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Nishijima et al Mol Biol Cell. 2017 Jun 15;28(12):1652-1

    Insert

    IFT74 (IFT74 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3-HA-GBF1(E794K)

    Plasmid

    #206416

    Purpose

    Mammalian expression of hGBF1(E794K)

    Depositor

    Kazuhisa Nakayama

    Article

    Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

    Insert

    GBF1 (GBF1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E794K

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. dmBACCS2-IRES-dOrai-IRES-GFP

    Plasmid

    #72893

    Purpose

    Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2-IRES-dOrai-IRES-GFP
    

    Tags

    IRES-GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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24. dmBACCS2NS-IRES-dOrai-IRES-GFP

    Plasmid

    #72895

    Purpose

    Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2NS-IRES-dOrai-IRES-GFP
    

    Tags

    IRES-GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Igfbp1-promoter/pGL3

    Plasmid

    #12146

    Depositor

    Domenico Accili

    Article

    Nakae et al EMBO J. 2000 Mar 1. 19(5):989-96.

    Insert

    IGFBP1 promoter (Igfbp1 Rat)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Contains the canonical insulin response sequence …

    Available Since

    Aug. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

26. pEGFP-C1-IFT27

    Plasmid

    #218722

    Purpose

    Expresses N-terminally EGFP-tagged IFT27 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

    Insert

    IFT27 (IFT27 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. 1037 pGL3 FasL promoter

    Plasmid

    #9028

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    Fas ligand promoter (FASLG Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

28. pcDNA4-Xpress-GBF1

    Plasmid

    #206417

    Purpose

    Mammalian expression of hGBF1

    Depositor

    Kazuhisa Nakayama

    Article

    Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

    Insert

    GBF1 (GBF1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 1129 pcDNA3 GFP FKHR H215R AAA

    Plasmid

    #9034

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    FKHR H215R AAA (FOXO1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    H215R T24A S256A S319A

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

30. mCherry-PHR-iSH-T2A-CIBNcaax

    Plasmid

    #173860

    Purpose

    Mammalian expression of mCherry-PHR-iSH-T2A-CIBNcaax (opto-PI3K)

    Depositor

    Takao Nakata

    Article

    Kakumoto et al PLoS One. 2013 Aug 7;8(8):e70861. doi: 1

    Insert

    mCherry-PHR-iSH-T2A-CIBNcaax
    

    Tags

    C-terminal CAAX-box and mCherry

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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