We narrowed to 1,270 results for: grna cloning vector

1. pKLV-EF1aGFP2AKAT7-W

   Plasmid

   #159292

   Purpose

   Lentiviral vector expressing GFP and KAT7 (gRNA-resistant) driven by human EF1a promoter

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   KAT7 (KAT7 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   CRISPR sites mutated (gRNA ID. 5 and A10)

   Promoter

   human EF1a promoter

   Available Since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLV-EF1aGFP2AKAT7-linker-mAID-W

   Plasmid

   #159294

   Purpose

   Lentiviral vector expressing GFP and KAT7 (gRNA-resistant, mAID-tagged) driven by human EF1a promoter

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   KAT7 (KAT7 Human)
   

   Use

   Lentiviral

   Tags

   mAID (auxin-inducible degron)-tagged

   Expression

   Mammalian

   Mutation

   CRISPR sites mutated (gRNA ID. 5 and A10)

   Promoter

   human EF1a promoter

   Available Since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-crRNA(TARDBP)

   Plasmid

   #109323

   Purpose

   AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TARDBP

   Depositor

   Feng Zhang

   Article

   Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

   Insert

   mCherry-KASH (TARDBP Human)
   

   Use

   AAV

   Promoter

   hSyn1

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiCRISPRv2GFP

   Plasmid

   #82416

   Purpose

   3rd generation lentiviral vector expressing GFP alongside Cas9 and an sgRNA cloning site

   Depositor

   David Feldser

   Article

   Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

   Insert

   GFP
   

   Use

   CRISPR and Lentiviral

   Promoter

   EFS (P2A)

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. AA151

   Plasmid

   #215938

   Purpose

   Fragmid fragment: (guide cassette) for dual expression sgRNA

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; BsmBI_v0; BsmBI_v4; rev{H1_v1}
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRRL-mU6-mScarletGO-P2A-Blas-PGK-Neo

   Plasmid

   #136904

   Purpose

   Lentivirus for base editing activatable mScarletI expression in mammalian cells. All-in-one vector with sgmScarletGO.

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   mScarletGo-p2a-Blas
   

   Use

   Lentiviral

   Mutation

   ATG>ACG

   Promoter

   SFFV

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPRyl

   Plasmid

   #70007

   Purpose

   CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2015 Dec 29.

   Inserts

   Codon optimized Cas9 from S. pyogenes
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available Since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPRa_VPR_yl

   Plasmid

   #107677

   Purpose

   CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertion

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al Biotechnol J. 2018 May 5:e1700584. doi:

   Inserts

   Codon optimized dCas9-VPR
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRRL-mU6-GFPGO2-IRES-mScarletI

   Plasmid

   #136896

   Purpose

   Lentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO.

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   GFPGO2-IRES-mScarletI
   

   Use

   Lentiviral

   Tags

   2xNLS on GFP, 3xNLS on mScarlet

   Mutation

   ATG>ACG

   Promoter

   SFFV

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRRL-mU6-GFPGO-IRES-mScarletI

    Plasmid

    #136895

    Purpose

    Lentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO2.

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    GFPGO-IRES-mScarletI
    

    Use

    Lentiviral

    Tags

    3xNLS on mScarlet

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRRL-mU6-GFPGO-IRES-mScarletI-PGK-Neo

    Plasmid

    #136897

    Purpose

    Lentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO.

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    GFPGO-IRES-mScarletI
    

    Use

    Lentiviral

    Tags

    3xNLS on mScarlet

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-mU6-Luc2GO-PGK-Neo

    Plasmid

    #136905

    Purpose

    Lentivirus for base editing activatable Luciferase2 expression in mammalian cells. All in one vector with sgLuc2GO.

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    Luc2CyGo
    

    Use

    Lentiviral

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti_MCP-VP64_Hygro

    Plasmid

    #138458

    Purpose

    lenti vector encoding the MS2-VP64 with a 2A Hygro resistance marker

    Depositor

    Jian Xu

    Article

    Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

    Insert

    vp64
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKM126

    Plasmid

    #105134

    Purpose

    Clostridium difficile CRISPR-cas9 mutagenesis plasmid Tn916 oriT

    Depositor

    Joseph Sorg

    Article

    McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

    Inserts

    Upstream & Downstream pyrE deletion region
    tetR
    Cas9
    gRNA
    

    Use

    E. coli - c. difficile shuttle vector

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCast

    Plasmid

    #200169

    Purpose

    E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme

    Depositor

    Yong-Su Jin

    Article

    Kuanyshev et al Biotechnol Bioeng. 2021 Jan;118(1):372-3

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-hU6-mU6-BlasGO

    Plasmid

    #136906

    Purpose

    Lentivirus for base editing activatable Blasticidin resistance gene expression in mammalian cells. All in one vector with sgBlasGO.

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    BlasCyGO
    

    Use

    Lentiviral

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. 2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO

    Plasmid

    #172225

    Purpose

    Cas9-2A-GFP expression vector bearing two sgRNAs targeting the centromeric human SOX17 CTCF-boundary.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. BoxB-MS2 adRNA (RAB7A-ACG site)

    Plasmid

    #170155

    Purpose

    AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript for C-U editing

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    BoxB-RAB7A(ACG)-MS2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. BoxB-MS2 adRNA (RAB7A-UAG site)

    Plasmid

    #170138

    Purpose

    AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    BoxB-RAB7A(UAG)-MS2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. 2X_pX458_pSpCas9(BB)-2A-GFP_NANOG_bKO

    Plasmid

    #172224

    Purpose

    Cas9-2A-GFP expression vector bearing two sgRNAs targeting the telomeric human NANOG CTCF-boundary.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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