We narrowed to 36,578 results for: KAN

Showing: 7381 - 7400 of 36578 results

Results per page:

Sort results by:

pFX-mCherry-Rab11

Plasmid

#174461

Purpose

Express mCherry with recycling endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with recycling endosome-targeting sequence

Tags

small GTPase (Rab11)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
L1_lacZgRNA-Ck2

Plasmid

#136137

Purpose

L1 in position 2, to clone gRNA target using BbsI, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpU6:lacZgRNA

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pmr101A-cPr-mCherry (KmR)

Plasmid

#177207

Purpose

Plasmid with p15A origin for constitutive mCherry expression in E. coli

Depositor

Alexey Merz

Insert

mCherry

Expression

Bacterial

Promoter

Synthetic constitutive promoter

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PtxS1-c003

Plasmid

#173076

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PtxS1

Expression

Bacterial

Mutation

D35-I221

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pN1-QUE37C

Plasmid

#129318

Purpose

ATP measurement

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN-37C

Expression

Mammalian

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET41sIII_GST-ALC1

Plasmid

#173932

Purpose

Bacterial expression of ALC1

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

ALC1 (CHD1L Human)

Tags

GST

Expression

Bacterial

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAT9651-BEAR-GFP

Plasmid

#162989

Purpose

BEAR target plasmid with split EGFP and disrupted 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-PLIN2-tdTOMATO

Plasmid

#172533

Purpose

PLIN2-tdTOMATO fusion (zebrafish lipid droplet reporter) in middle entry vector for Gateway cloning

Depositor

Richard White

Article

Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

Insert

plin2-tdtomato

Mutation

S80R, A81S, E144D, I148F, and R415H mutations in …

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-SLCO1B1_STOP

Plasmid

#161354

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLCO1B1 (SLCO1B1 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
p3E-TurboID

Plasmid

#166570

Purpose

Multisite Gateway 3' entry clone for fusions onto the N-terminus of TurboID. Parton lab clone JSS

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID

Use

Gateway Cloning

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-NES-SspB-MCS-P2A-iLIDcaax

Plasmid

#173869

Purpose

Mammalian expression of mCherry-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-MCS-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-EGFP (no signal control)

Plasmid

#170167

Purpose

Mammalian Expression of no signal control

Depositor

Christopher Moxham

Type

Empty backbone

Expression

Mammalian

Mutation

n/a

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSF279

Plasmid

#167674

Purpose

Intermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pK27Sumo_His-SUMO-nsp8 (SARS-CoV-2)

Plasmid

#169187

Purpose

To express His-SUMO-nsp8 (SARS-CoV-2) in E. coli

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

14His-SUMO-nsp8 (ORF1ab SARS-CoV-2, Synthetic)

Tags

14His-SUMO (Ulp1-cleavable)

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T5

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
LiPOP1

Plasmid

#168264

Purpose

A single-component optogenetic tool for photoswitchable necroptosis

Depositor

Yubin Zhou

Article

He et al Adv Sci (Weinh). 2021 May 6;8(13):210042

Insert

MLKL(1-125) (MLKL Human)

Tags

mCherry, CRY2-PHR

Expression

Mammalian

Mutation

H15A, K16A, R17A

Promoter

CMV

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJCX4

Plasmid

#170756

Purpose

Express in mammalian cells a target protein C-terminally fused to the Mxe GryA intein - chitin binding domain (CBD) tag.

Depositor

Roberto Dominguez

Article

Carman et al J Biol Chem. 2021 Oct;297(4):101154. doi

Type

Empty backbone

Tags

Mxe GyrA intein - chitin binding domain (CBD)

Expression

Mammalian

Promoter

CMV

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EPS15-GFP-His

Plasmid

#170860

Purpose

Mammalian expression of EPS15-GFP-His.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Epidermal growth factor receptor pathway substrate 15 (EPS15) (EPS15 Human)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVA221AID-T7RNAP

Plasmid

#167977

Purpose

Expresses fusion protein AID-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

AID-T7RNAP

Tags

T7 tag and T7RNAP

Expression

Bacterial

Promoter

TetR-Ptet

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-GATA4

Plasmid

#101417

Purpose

Donor Vector containing GATA4 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

GATA4 (GATA4 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-StrepII (Human autophagy E3-like enzyme)

Plasmid

#169076

Purpose

Insect codon optimized ATG genes (excluded tags):10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-TEV-StrepII

Depositor

Sascha Martens

Article

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Inserts

ATG5 (ATG5 Human)
ATG7 (ATG7 Human)
ATG10 (ATG10 Human)
ATG12 (ATG12 Human)
ATG16 (ATG16L1 Human)

Tags

GGlinker, TEV cleavage site, StrepII and HIS10 Ta…

Expression

Bacterial and Insect

Promoter

Polyhedrin promoter

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge