19,660 results

1. GSK3 beta pGEX

   Plasmid

   #15898

   Depositor

   Jim Woodgett

   Insert

   GSK3 beta (GSK3B Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Nov. 1, 2007

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. Flag-HA-USP8

   Plasmid

   #22608

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP8 (USP8 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Dec. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCEV-G1-Km

   Plasmid

   #46813

   Purpose

   TEF1 and PGK1 promoter controlled expression cassettes with G418 resistance for yeast transformation

   Depositor

   Lars Nielsen, Claudia Vickers

   Article

   Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

   Type

   Empty backbone

   Tags

   FLAG and c-myc

   Expression

   Yeast

   Promoter

   TEF1 and PGK1

   Available Since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO5d.SFFV.dCas9-KRAB.P2A.BSD

   Plasmid

   #90332

   Purpose

   CRISPR interference

   Depositor

   Dirk Heckl

   Article

   Schwarzer et al Nat Commun. 2017 Aug 9;8(1):218. doi: 10

   Inserts

   dCas9
   na
   SFFV
   P2A-BSD
   

   Use

   Lentiviral

   Tags

   HA

   Available Since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pY108 (lenti-AsCpf1)

   Plasmid

   #84739

   Purpose

   Lenti virus delivery of AsCpf1 and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huAsCpf1
   puromycin resistance gene
   

   Use

   Lentiviral

   Tags

   3xHA, NLS, and P2A

   Promoter

   EFS

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOPINB-AMSH*

   Plasmid

   #66712

   Purpose

   Expresses activated AMSH in E. coli

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

   Inserts

   hbp (Stam2 Mouse)
   AMSH (STAMBP Human)
   

   Tags

   His-3C

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

7. HA-AMOT p130

   Plasmid

   #32821

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

   Insert

   AMOT p130 (AMOT Human)
   

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   E5D

   Promoter

   CMV

   Available Since

   Jan. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQE80L-IL2 C125S

   Plasmid

   #104348

   Purpose

   Expresses human IL-2 (C125S)

   Depositor

   Alexander McLellan

   Insert

   Human IL-2 (C125S) (IL2 Human)
   

   Tags

   MRGSHHHHHGS-tag

   Expression

   Bacterial

   Mutation

   C125S mutation (TGT -> TCC)

   Promoter

   T5

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV.rTH.PI.Cre.SV40

   Plasmid

   #107788

   Purpose

   AAV vector expressing Cre from rTH promoter

   Depositor

   James M. Wilson

   Has Service

   AAV Retrograde and AAV9

   Insert

   Cre
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   rTH

   Available Since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. GADD45 WT-luc

    Plasmid

    #8356

    Depositor

    Michael Greenberg

    Article

    Tran et al Science 2002 Apr 19;296(5567):530-4.

    Insert

    GADD45 promoter WT (GADD45A Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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11. hTLR4

    Plasmid

    #13086

    Depositor

    Ruslan Medzhitov

    Insert

    TLR4 (TLR4 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCGN N-Ras wt

    Plasmid

    #14723

    Depositor

    Adrienne Cox

    Article

    Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

    Insert

    N-ras (NRAS Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Aug. 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVS1-idCas9-vpr

    Plasmid

    #89985

    Purpose

    inducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locus

    Depositor

    Jie Na

    Article

    Guo et al Protein Cell. 2017 Jan 23. doi: 10.1007/

    Insert

    dCas9-VPR
    

    Expression

    Mammalian

    Promoter

    TRE-tight

    Available Since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. CaV1.2

    Plasmid

    #26572

    Depositor

    Diane Lipscombe

    Article

    Helton et al J Neurosci. 2005 Nov 2. 25(44):10247-51.

    Insert

    Calcium channel, voltage-dependent, L type, alpha 1C subunit (Cacna1c Mouse)
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. Gi3-CASE

    Plasmid

    #168122

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAI2, GNB1, GNG2 (GNAI1, GNB1, GNG2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG2

    Expression

    Mammalian

    Mutation

    NLuc is inserted at A114/E115 within GNAI1

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGWB517

    Plasmid

    #74859

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEX_306

    Plasmid

    #41391

    Purpose

    Constitutive lentiviral expression, SV40-puro; PGK-gateway-V5 tag

    Depositor

    David Root

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSCV-human Erbb2-IRES-GFP

    Plasmid

    #91888

    Purpose

    Retroviral expression of human Erbb2

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    Erbb2 (ERBB2 Human)
    

    Use

    Retroviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Promoter

    MMLV gag

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWZL Neo Myr Flag ADRBK1

    Plasmid

    #20418

    Depositor

    William Hahn, Jean Zhao

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    ADRBK1 (GRK2 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Available Since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

20. Fuw-dCas9-Tet1CD_IM

    Plasmid

    #84479

    Purpose

    Lentiviral construct to express dCas9 fused with an inactive catalytic domain of Tet1 (H1672Y, D1674A)

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Tet1CD_IM
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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