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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 28,416 results for: REP

Showing: 7461 - 7480 of 28416 results

  1. PB-TAC-Cas9

    Plasmid
    #120352
    Purpose
    piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). SpCas9 is constitutively expressed.
    Depositor
    Knut Woltjen
    Article
    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tet-on and CBh
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    120352-plasmid-map-sequence-id-237238

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  2. PB-TAC-KRAB-dCas9

    Plasmid
    #120355
    Purpose
    piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). KRAB-dCas9 is constitutively expressed.
    Depositor
    Knut Woltjen
    Article
    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tet-on and CBh
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    120355-plasmid-map-sequence-id-237262

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  11. DT960

    Plasmid
    #80412
    Purpose
    Expresses 960 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats
    Depositor
    Thomas Cooper
    Article
    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub
    Insert
    human DMPK exons 11-15 with 960 interrupted CTG repeats (DMPK Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    80412-plasmid-map-sequence-id-151397

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  13. pSL103-SpCas9-FLAG-3XNLS

    Plasmid
    #182032
    Purpose
    Expression of recombinant Streptococcus pyogenes Cas9 protein carrying C-terminal FLAG and 3XNLS for genome editing as Cas9 RNP
    Depositor
    Steven Lin
    Article
    Lin et al Bio Protoc. 2022 May 20;12(10):e4420. do
    Insert
    Streptococcus pyogenes cas9 (cas9 Synthetic)
    Use
    CRISPR
    Tags
    12X His tag, 3XNLS, FLAG, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    182032-plasmid-map-sequence-id-360101

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  14. DT480

    Plasmid
    #80413
    Purpose
    Expresses 480 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats
    Depositor
    Thomas Cooper
    Article
    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub
    Insert
    human DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    80413-plasmid-map-sequence-id-151399

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  16. pCB578

    Plasmid
    #141095
    Purpose
    E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array
    Depositor
    Chase Beisel
    Article
    Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d
    Inserts
    Cas9 and tracrRNA
    repeat-spacer-repeat array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    141095-plasmid-map-sequence-id-292312

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  17. UAS:mCherry

    Plasmid
    #170994
    Purpose
    UAS:GAL4 repeat-containing promoter drives simple mCherry reporter gene. Blasticidin selectable.
    Depositor
    Alice Ting
    Article
    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:
    Insert
    UAS:mCherry
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Synthetic 14x UAS:Gal4
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    170994-plasmid-map-sequence-id-335660

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  20. pTiGc

    Plasmid
    #78314
    Purpose
    Mycobacterial dual reporter plasmid for fluorescence dilution. Description: hsp60-gfp, hsp60(ribo)-turboFP635 (inducible TurboFP635 under control of theophylline - inducible riboswitch).
    Depositor
    Samantha Sampson
    Article
    Mouton et al Microbiology. 2016 Mar 30. doi: 10.1099/
    Inserts
    TurboFP635
    GFP
    Use
    Synthetic Biology; Mycobacterial replicating vect…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    hsp60 and hsp60(ribo)
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 7461 - 7480 of 28416 results