We narrowed to 22,711 results for: Sis;

1. pJL1-aadA

   Plasmid

   #117050

   Purpose

   In vitro expression of aadA (streptomycin resistance gene) from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

   Insert

   aadA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHH0103_STAM-1/1

   Plasmid

   #91428

   Purpose

   Protein expression and purification of human SH3 domain construct STAM-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   STAM-1/1 (STAM Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC 3GLA UAS HAi

   Plasmid

   #71763

   Purpose

   gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant, with 5xUAS and HA tag

   Depositor

   Matthias Soller

   Article

   Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. sh-Myocardin

   Plasmid

   #100768

   Purpose

   expression of shRNA targeting MYOCD

   Depositor

   Joseph Gordon

   Article

   Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

   Insert

   sh-Myocardin
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   H1

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHH0103_PACSIN3-1/1

   Plasmid

   #91335

   Purpose

   Protein expression and purification of human SH3 domain construct PACSIN3-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   PACSIN3-1/1 (PACSIN3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. RAB14 3'UTR D1

   Plasmid

   #61490

   Purpose

   luciferase reporter containing RAB14 3' UTR with the first predicted MiR144 binding site deleted

   Depositor

   Curt Civin

   Article

   Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

   Insert

   RAB14 3' UTR (RAB14 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   contains position 837-1637 (numbering relative to…

   Promoter

   PGK

   Available Since

   Jan. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pOT2 3GLA

   Plasmid

   #71764

   Purpose

   gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, chloramphenicol resistant

   Depositor

   Matthias Soller

   Article

   Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMath3-luc

   Plasmid

   #51312

   Purpose

   Luciferase reporter driven by the Math3 promoter

   Depositor

   Ryoichiro Kageyama

   Article

   Tsuda et al J Biol Chem. 1998 Mar 13;273(11):6327-33

   Insert

   Math3 promoter (-2.8k- +196) (Neurod4 Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mutation

   Promoter

   Available Since

   March 7, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pEG302 5aa SunTag VP64 gRNA17 (FWA)

   Plasmid

   #115482

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g17_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDF1-ERBB4 (E563K)

    Plasmid

    #29541

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

    Insert

    ERBB4 (ERBB4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pBT307_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PhiC31o-pA-FRT5

    Plasmid

    #52540

    Purpose

    Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attPx3 (70 bp each)
    Neo
    PhiC31o integrase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBabe hygro NFS1 CD

    Plasmid

    #102978

    Purpose

    Expression of NFS1 C381A mutant

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    NFS1 C381A (NFS1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    C381A catalytic site mutant, Silent mutations to …

    Promoter

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

13. pDESTFLAGHApuro3-Ago2Q545AK570A

    Plasmid

    #14105

    Depositor

    Thomas Tuschl

    Article

    Ma et al Nature. 2005 Mar 31. 434(7033):666-70.

    Insert

    Ago2 (AGO2 Human)
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    changed Gln545 to Ala and Lys570 to Ala

    Promoter

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pMf3tvtaulacZ

    Plasmid

    #37264

    Depositor

    Patricia Labosky

    Article

    Kloetzli et al Genesis. 2001 Feb;29(2):60-71.

    Insert

    Foxb1 (Foxb1 Mouse)
    

    Use

    Tags

    taulacZ

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pCDNA3 Flag BimL (S44D)

    Plasmid

    #24235

    Depositor

    Roger Davis

    Article

    Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

    Insert

    BimL (S44D) (BCL2L11 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed serine 44 to aspartic acid

    Promoter

    Available Since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pIS47 C20orf139 3'UTR mut

    Plasmid

    #14490

    Depositor

    David Bartel

    Article

    Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

    Insert

    C20orf139 3'UTR mutant (SRXN1 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    CATTCC’s were changed to AAGTAC's

    Promoter

    Available Since

    March 7, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL2-MCT2Prom4.2

    Plasmid

    #87347

    Purpose

    Human monocarboxylate transporter 2 (MCT2) promoter 4.2 kbp

    Depositor

    Saroj Mathupala

    Article

    Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

    Insert

    MCT2 (SLC16A7 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    4.2 kbp proximal promoter

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET24a_UDP_Xase_E113

    Plasmid

    #73159

    Purpose

    Expresses in E. coli, S. pneumoniae TIGR4 UDP-Xase SP_1669 mutant E113A

    Depositor

    Sandra Gabelli

    Article

    Duong-Ly et al PLoS One. 2013 May 15;8(5):e64241. doi:

    Insert

    pET24a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    change glutamate 113 to alanine

    Promoter

    Available Since

    March 22, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-HA-REDD1 C140S (#622)

    Plasmid

    #99947

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C140S

    Promoter

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET151-Vta1(280-330) S278C

    Plasmid

    #87739

    Purpose

    Bacterial expression of a VSL domain variant of S. cerevisiae Vta1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-277, mutated Ser 278 to Cys

    Promoter

    T7

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3-FLAG/MKRN1(110-482)

    Plasmid

    #78754

    Purpose

    To overexpress MKRN1(110-482) in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

    Insert

    MKRN1 (MKRN1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    110-482

    Promoter

    CMV

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pNUT N6His mICA NG

    Plasmid

    #70162

    Purpose

    Expresses N-His tagged nonglycosylated mouse inhibitor of carbonic anhydrase

    Depositor

    Ross MacGillivray, Anne Mason

    Article

    Wang et al Biochem J. 2007 Aug 15;406(1):85-95.

    Insert

    mouse inhibitor of carbonic anhydrase
    

    Use

    Tags

    N-terminal signal peptide, 4 aa link, 6 His, Fact…

    Expression

    Mammalian

    Mutation

    Asn470Asp and Asn645Asp

    Promoter

    SV40

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p5A7-T

    Plasmid

    #92020

    Purpose

    Expresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain and a second in the C-terminal domain, anhydrotetracycline inducible TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting lac operator with N and C-terminal TEV protease cleavage sites
    Tobacco Etch Virus Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Expression

    Mutation

    S219V

    Promoter

    Available Since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p5A3-T

    Plasmid

    #92019

    Purpose

    Expresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting lac operator with N-terminal TEV cleavage site
    Tobacco Etch Virus Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Expression

    Mutation

    S219V

    Promoter

    Available Since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET151-Vps4(81-437)

    Plasmid

    #87734

    Purpose

    Bacterial expression of N-terminally truncated S. cerevisiae Vps4

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-80

    Promoter

    T7

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBGO

    Plasmid

    #62671

    Purpose

    Donor vector for mFTH1 knock-in via landing pad

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

    Insert

    FTH1
    

    Use

    Cre/Lox

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-HA-RIPK3 V460P

    Plasmid

    #78814

    Purpose

    To overexpress RIPK3 V460P in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    V460P

    Promoter

    CMV

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGB1-mCRT(P-domain)

    Plasmid

    #83519

    Purpose

    Bacterial expression of Calreticulin mutant

    Depositor

    Malini Raghavan

    Article

    Wijeyesakere et al J Immunol. 2016 May 1;196(9):3896-909. d

    Insert

    CALR (Calr Mouse)
    

    Use

    Tags

    GB1 and His

    Expression

    Bacterial

    Mutation

    P domain (contains residues 187-283)

    Promoter

    T7

    Available Since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

29. pGB1-mCRT(C-domain)

    Plasmid

    #83518

    Purpose

    Bacterial expression of Calreticulin mutant

    Depositor

    Malini Raghavan

    Article

    Wijeyesakere et al J Immunol. 2016 May 1;196(9):3896-909. d

    Insert

    CALR (Calr Mouse)
    

    Use

    Tags

    GB1 and His

    Expression

    Bacterial

    Mutation

    C domain (contains residues 340-399)

    Promoter

    T7

    Available Since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

30. pCDNA3 Flag BimL (S44A)

    Plasmid

    #24234

    Depositor

    Roger Davis

    Article

    Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

    Insert

    BimL (S44A) (BCL2L11 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed Serine 44 to Alanine

    Promoter

    Available Since

    June 1, 2010

    Availability

    Academic Institutions and Nonprofits only