We narrowed to 13,006 results for: Hal

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pFUW-TetO-MXD1

Plasmid

#178443

Purpose

doxycycline-inducible expression of Human MXD1 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

MXD1 (MXD1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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Bact2-APEX2-GBP in pDEST-Tol2-pA2-acrys-cherry

Plasmid

#67668

Purpose

Zebrafish expression vector for ubiquitous expression of APEX2-GBP

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Use

Zebrafish expression with tol2

Available Since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pCCM

Plasmid

#162708

Purpose

Second CCM operon cloned from H. neapolitanus; pFA expressing the remaining 11 genes in the H. neapolitanus CCM cluster

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

Second CCM operon cloned from H. neapolitanus

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-N-G204R

Plasmid

#177953

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (G204R). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (G204R) (N )

Expression

Mammalian

Mutation

G204R

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET14b-dabAB2

Plasmid

#133013

Purpose

pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a c-terminal strep tag fusion and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

dabB2
dabA2

Tags

6xHis, StrepII tag, and sfGFP

Expression

Bacterial

Promoter

t7

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV EFIa DIO LMO11 (GeNL_SS-ChRmine)

Plasmid

#219426

Purpose

Cre-dependent expression of luciferase opsin fusion protein (luminopsin) for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Ikefuama et al iScience. 2025 Feb 13;28(3):112022. doi:

Insert

mNeonGreen, ISN-IE, ChRmine

Use

AAV

Tags

Gaussia luciferase signal sequence

Promoter

EF-1alpha

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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CLPIT Cry2PHR-mCh-RhoA

Plasmid

#42959

Depositor

David Schaffer

Article

Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

Insert

Cry2PHR-mCherry-RhoA (RHOA Mustard Weed, Human)

Use

Retroviral

Expression

Mammalian

Promoter

Tet-Off promoter

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pSL0812 (pCRISPR(BbsI)_Vch)

Plasmid

#200898

Purpose

Expresses V. cholerae CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchCRISPR (entry)

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pEY26

Plasmid

#191031

Purpose

cho-1(3.3-2.6k) fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

cho-1(3.3-2.6k) (cho-1 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEY39

Plasmid

#191043

Purpose

acr-2(2.1k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

acr-2(2.1k) (acr-2 Nematode)

Expression

Worm

Mutation

None

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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optoPKAW196R/Y204A

Plasmid

#104546

Purpose

Expresses Cry2-mCherry-PKA W196R/Y204A in mammalian cells.

Depositor

David Lawrence

Article

O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

Insert

Cry2-mCherry-PKA W196R/Y204A

Tags

mCherry

Expression

Mammalian

Mutation

PKA W196R and Y204A

Promoter

CMV

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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pEBG-PELP1

Plasmid

#65209

Purpose

Full length mammalian expression vector for hPELP1

Depositor

Ratna Vadlamudi

Article

Nair et al Cancer Res. 2010 Sep 15;70(18):7166-75.

Insert

PELP1 (PELP1 Human)

Tags

GST

Expression

Mammalian

Promoter

EF1a

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-S109A

Plasmid

#33088

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 )

Tags

Flag

Expression

Mammalian

Mutation

S109A

Promoter

CMV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EYFP

Plasmid

#167898

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-RFP(pLxIS-PLPLR)

Plasmid

#221284

Purpose

Expression of RFP (pLxIS-PLPLR) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

RFP (pLxIS-PLPLR) (Mkrn1 Synthetic)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-TRIF(Nterm)

Plasmid

#221244

Purpose

Expression of TRIF N-terminus (1-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF N-terminus (1-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-flex-GFP-shRNA-scramble

Plasmid

#182503

Purpose

Expresses scramble shRNA for control expts. Flexed cassette driven by pan neuronal gene promoter

Depositor

William Wisden

Article

Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

Insert

pan neuronal promoter driving flexed GFP-shRNA scramble from mir 30 cassette

Use

AAV, Cre/Lox, and RNAi

Expression

Mammalian

Promoter

hSyn promoter

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37a, 46]

Plasmid

#26569

Depositor

Diane Lipscombe

Article

Bell et al Neuron. 2004 Jan 8. 41(1):127-38.

Insert

calcium channel alpha-1B subunit (Cacna1b Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

Deletion of exons a10, 18a, and 24a. Contains …

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pME-CreLite

Plasmid

#131782

Purpose

Middle entry vector (Tol2 kit) with bi-cistronic CreLite system components, PhyBΔCreC and PIF6CreN, linked by P2A.

Depositor

George Eisenhoffer

Article

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Insert

PhyBCreC-P2A-PIF6CreN

Use

Cre/Lox and Gateway Cloning

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pJY-RpABE-PDS3_IMS

Plasmid

#112875

Purpose

binary vector for IMS (Induced Mis-Splicing) of PDS3 in Arabidopsis

Depositor

Jin-Soo Kim, Jae-Young Yun

Article

Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

Inserts

RPS5A promoter
ABE7.10
U6-sgRNA targeting PDS3

Use

CRISPR

Expression

Plant

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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