We narrowed to 8,578 results for: sgRNA
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Plasmid#139457PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-NCOA7g2 (BB23)
Plasmid#139458PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgGAL4-4
Plasmid#46916PurposeHuman pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter (negative control)DepositorInsertssgGAL4-4
Puromycin resistance and mCherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMV and U6Available sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-pegRNA_KCNA1-EGFP
Plasmid#159787PurposeS. pyogenes pegRNA for C to A mutation at the KCNA1 site of human cells using prime editingDepositorInsertspacer of pegRNA targeting KCNA1 gene (KCNA1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Puro-H3C2
Plasmid#207783PurposeDonor template for moxGFP-2A-Puro insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780DepositorInsertH3C2 Homology Arms flanking a moxGFP-Puro Cassette (H3C2 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJMP1217
Plasmid#119258Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast SHMT2 CD
Plasmid#106302PurposeExpresses SHMT2 K280A catalytic site mutantDepositorInsertserine hydroxymethyltransferase 2 (SHMT2 Human)
UseRetroviralTagsExpressionMammalianMutationK280A catalytic site mutation, Silent mutations d…PromoterAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRCreb5gRNA
Plasmid#195021PurposeSequence specific sgRNA that guide Cas9 to the genomic region encoding the bovine Creb5 DNA binding domainDepositorInsertCreb5 gRNA (CREB5 Bovine)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMP1069
Plasmid#119244Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-AttL1-NotI/BamHI-U6-BsaI-tracrRNAopt-BamHI-U6-SapI-tracrRNAopt-NotI/XbaI-7sk-BsmBI-tracrRNA-XbaI-AttL2
Plasmid#190898PurposeEntry vector for multiple sgRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and MammalianMutationPromoterU6 and 7skAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Hygro-sgSIK3
Plasmid#138699PurposeExpresses a human SIK3-targeting sgRNA and Cas9DepositorInsertsgSIK3 human (SIK3 Human)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Puro-SIK1
Plasmid#138695PurposeExpresses a human SIK1-targeting sgRNA and Cas9DepositorInsertsgSIK1 human (SIK1 Human)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Blast-sgSIK2
Plasmid#138708PurposeExpresses a human SIK2-targeting sgRNA and Cas9DepositorInsertsgSIK2 human (SIK2 Human)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJL1-mRFP1 gRNA
Plasmid#117052PurposeIn vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoterDepositorInsertanti-mRFP1 SpCas9 gRNA
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1071
Plasmid#119245Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Blast-sTagRFP-TUBA1B
Plasmid#207768PurposeDonor template for Blast-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763DepositorInsertTUBA1B Homology Arms flanking a Blast-sTagRFP Cassette (TUBA1B Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSLQ7780 mU6:- || CMV: mCherry~P2A-DD-AcrIIA4
Plasmid#123658PurposeDD-AcrIIA4 fusion for Shield1-mediated AcrIIA4 control without sgRNA following mU6 promoter.DepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromotermU6Available sinceApril 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1290
Plasmid#119267Purposeconstruction intermediateDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1067
Plasmid#119243Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
Direct-seq_lentiGuide-Puro-tRNA-Tail-8A8G
Plasmid#157984PurposeExpresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.DepositorInsertS. pyogenes sgRNA cassette (tRNA-Tail-8A8G)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only