19,660 results

1. UAS-mCherry

   Plasmid

   #135457

   Purpose

   Mammalian mCherry reporter to read out FLARE and SPARK activation

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   mCherry
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   UAS

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV4-p52

   Plasmid

   #23289

   Depositor

   Warner Greene

   Article

   Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

   Insert

   p52 (NFKB2 Human)
   

   Expression

   Mammalian

   Available Since

   March 31, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-parkin C431S

   Plasmid

   #45877

   Depositor

   Edward Fon

   Article

   Trempe et al Science. 2013 May 9.

   Insert

   Parkin (PRKN Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C431S mutation (ubiquitin acceptor site)

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pCE059-SiT-Cas12a-[Activ]

   Plasmid

   #128136

   Purpose

   Express both AsCas12a fused to [Activ] activator domain and CRISPR arrays on a single transcript

   Depositor

   Randall Platt

   Article

   Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

   Insert

   AsCas12a-[Activ]-Triplex
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCSII-EF-miRFP709-hCdt(1/100)

   Plasmid

   #80007

   Purpose

   hCdt(1/100) fused with near-infrared fluorescent protein miRFP709 (G1 phase cell cycle reporter)

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

   Insert

   hCdt1(1/100) (CDT1 Human)
   

   Use

   Lentiviral

   Tags

   miRFP709

   Promoter

   EF-1 alpha

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-Tre3G-47xCAG-12xMS2

   Plasmid

   #99148

   Purpose

   Encodes 47xCAG repeats with 12xMS2 tag, 2nd generation lentiviral vector

   Depositor

   Ron Vale

   Article

   Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

   Insert

   47xCAG 12xMS2 hairpins
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKIR1.1

   Plasmid

   #85758

   Purpose

   CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Plant

   Promoter

   AtRPS5A

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. MigR1-AE9a

   Plasmid

   #12433

   Depositor

   Dong-Er Zhang

   Article

   Yan et al Nat Med. 2006 Aug . 12(8):945-9.

   Insert

   AML1-ETO9a fusion (RUNX1, RUNX1T1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   AML-ETO protein with ETO exon 9a.

   Available Since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. NanoLuc-PM

   Plasmid

   #164784

   Purpose

   Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors

   Depositor

   Laszlo Hunyady

   Article

   Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

   Insert

   Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Oplophorus gracilirostris, Human)
   

   Tags

   NanoLuc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-sh1368 knockdown c-myc

    Plasmid

    #29435

    Depositor

    Bob Eisenman

    Article

    Lin et al EMBO J. 2009 Oct 21. 28(20):3157-70.

    Insert

    c-Myc shRNA (MYC Human)
    

    Use

    Lentiviral and RNAi

    Tags

    GFP and WPRE

    Expression

    Mammalian

    Available Since

    May 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5_FRT_GFP

    Plasmid

    #127108

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    GFP
    

    Expression

    Mammalian

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKO403_lacZ_Cm

    Plasmid

    #174725

    Purpose

    Bifidobacterium-E.coli shuttle vector for seamless gene cloning

    Depositor

    Tohru Suzuki

    Article

    Altaib et al Microbiol Resour Announc. 2022 Jan 20;11

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-myc-Atg7

    Plasmid

    #24921

    Depositor

    Toren Finkel

    Article

    Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

    Insert

    Atg7 (ATG7 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDT-sgRNA

    Plasmid

    #138271

    Purpose

    Dual-targeting sgRNA vector that contains both a sgRNA against BFP (sgBG) and a sgRNA for a genomic target site (sgTS)

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

    Insert

    sgBG, sgTS
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. HA-HA-BI-AR

    Plasmid

    #171234

    Purpose

    Mammalian expression of 2xHA-tagged human androgen receptor (hAR)

    Depositor

    Steven Balk

    Article

    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

    Insert

    2xHA-tagged human androgen receptor (AR Human)
    

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRC_RR_VP1_r1c3

    Plasmid

    #65724

    Purpose

    Rescued infectious variant from pAAV2_RaPID library; mCherry insertion in place of the deleted amino acid sequence 453-GTTTQSR

    Depositor

    Junghae Suh

    Article

    Judd et al Mol Ther Nucleic Acids. 2012 Nov 13;1:e5

    Insert

    mCherry
    

    Use

    AAV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOSIP-KL (KanR, Lambda)

    Plasmid

    #45984

    Depositor

    Drew Endy, Keith Shearwin

    Article

    St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMRE145

    Plasmid

    #118497

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mScarlet-I
    

    Expression

    Bacterial

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV CMV-dSaCas9-KRAB-bGHpA

    Plasmid

    #106219

    Purpose

    AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

    Insert

    dead S. aureus Cas9 KRAB
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    D10A and N580A

    Promoter

    CMV

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-N1 human cofilin WT

    Plasmid

    #50859

    Purpose

    expresses human cofilin fused to EGFP in mammalian cells

    Depositor

    James Bamburg

    Article

    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

    Insert

    cofilin 1 (CFL1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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