165,688 results

1. pHel3

   Plasmid

   #102961

   Purpose

   E.coli/H.Pylori shuttle vector

   Depositor

   Rainer Haas

   Article

   Heuermann et al Mol Gen Genet. 1998 Mar;257(5):519-28.

   Type

   Empty backbone

   Use

   Shuttle vector

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1(+) Flag-His-ATM kd

   Plasmid

   #31986

   Depositor

   Michael Kastan

   Article

   Canman et al Science. 1998 Sep 11;281(5383):1677-9.

   Insert

   ATM kd (ATM Human)
   

   Tags

   Flag and His

   Expression

   Mammalian

   Mutation

   3'UTRf 9 nucl/D2870A N2875k. catalytically …

   Available Since

   March 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AAVS1_gRNA

   Plasmid

   #196139

   Purpose

   gRNA targeting the AAVS1 locus in a third generation Cas9 backbone with GFP

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   AAVS1 gRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTarget_CAST

   Plasmid

   #127926

   Purpose

   Target plasmid containing PSP1

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   PSP1 target (GGTT PAM)
   

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pESC-MET

   Plasmid

   #222482

   Purpose

   Co-expression of two genes in yeast with FLAG and MYC tags and MET15 selection

   Depositor

   Tetsuo Matsuzaki, Kiyofumi Yamada

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Tags

   FLAG and Myc

   Expression

   Yeast

   Available Since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pESC-LYS

   Plasmid

   #222483

   Purpose

   Co-expression of two genes in yeast with FLAG and MYC tags and LYS2 selection

   Depositor

   Tetsuo Matsuzaki, Kiyofumi Yamada

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Tags

   FLAG and Myc

   Expression

   Yeast

   Available Since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEF1α-Cre-2A-moxGFP

   Plasmid

   #207744

   Purpose

   Expression of the Cre recombinase linked to a moxGFP fluorescent marker

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   Cre-PGK-moxGFP
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pMXS-GshF-blasticidin

   Plasmid

   #210313

   Purpose

   Expresses humanized Streptococcus thermophilus GshF in mammalian cells

   Depositor

   Kivanc Birsoy

   Article

   Wang et al Nature. 2021 Nov;599(7883):136-140. doi:

   Insert

   Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
   

   Use

   Retroviral

   Tags

   FLAG peptide

   Mutation

   Codon optimized for expression in human cells

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Human Parkin (1-465)

   Plasmid

   #110758

   Purpose

   Bacterial expression of human Parkin

   Depositor

   David Komander

   Article

   Wauer et al EMBO J. 2013 Jul 31;32(15):2099-112. doi

   Insert

   PRKN (PRKN Human)
   

   Tags

   N-His6-GST-3C

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-hSyn-ASAP3-WPRE

    Plasmid

    #132331

    Purpose

    expresses ASAP3(ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

    Depositor

    Michael Lin

    Article

    Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

    Insert

    ASAP3
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSynapsin

    Available Since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1 acGFP

    Plasmid

    #128047

    Purpose

    Mammalian expression vector with N terminal AcGFP

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Tags

    AcGFP

    Expression

    Mammalian

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. gRNA_Cloning Vector BbsI

    Plasmid

    #128433

    Purpose

    Embty backbone vector for expression of gRNA for spCas9 genome editing or epigenome editing

    Depositor

    Izuho Hatada

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HCA2-Tango

    Plasmid

    #66396

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    HCA2 (HCAR2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHAGE-ESR1-Y537N

    Plasmid

    #116373

    Purpose

    Lentiviral expression of ESR1 Y537N

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ESR1 (ESR1 Human)
    

    Use

    Lentiviral

    Mutation

    Y537N

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1i

    Plasmid

    #233080

    Purpose

    Expression vector for sgRNA-S1b and eCas12f1i

    Depositor

    Kyoungmi Kim

    Article

    Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

    Insert

    U6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1i-2xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xFLAG

    Expression

    Mammalian

    Promoter

    hU6, CBh

    Available Since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.4_7C11-1 light chain

    Plasmid

    #198252

    Purpose

    recombinant expression of light chain of monoclonal anti-AMP antibody 7C11-1

    Depositor

    Aymelt Itzen

    Article

    Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

    Insert

    light chain of monoclonal anti-AMP antibody 7C11-1 (mouse kappa)
    

    Tags

    IL10 signal peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.4_7C11-1 heavy chain

    Plasmid

    #198251

    Purpose

    recombinant expression of heavy chain of monoclonal anti-AMP antibody 7C11-1

    Depositor

    Aymelt Itzen

    Article

    Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

    Insert

    heavy chain of monoclonal anti-AMP antibody 7C11-1 (mouse IgG2a)
    

    Tags

    IL10 signal peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP-APEX2-NIK3x

    Plasmid

    #129274

    Purpose

    Expression of APEX2 in the nucleolus

    Depositor

    Alice Ting

    Article

    Fazal et al Cell. 2019 Jun 14. pii: S0092-8674(19)30

    Insert

    GFP-APEX2-NIK3x
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. luxB:cp157Venus+luxA/pRSETB Duet

    Plasmid

    #197583

    Purpose

    BRET lux luciferase with cp157Venus for bacterial expression

    Depositor

    Takeharu Nagai

    Article

    Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

    Insert

    LuxA LuxBcp157Venus
    

    Use

    E.coli expression

    Tags

    His X 6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AIO-Puro

    Plasmid

    #74630

    Purpose

    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing

    Depositor

    Steve Jackson

    Article

    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Puromycin resistant marker

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    May 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pmCherry-C1 actin-3XNLS P2A mCherry

    Plasmid

    #58475

    Purpose

    Expresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

    Inserts

    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDONR223_FYN_WT

    Plasmid

    #82211

    Purpose

    Gateway Donor vector containing FYN , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    FYN (FYN Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. epB_UbC_CymRV5-nls-GFP_DEx2

    Plasmid

    #119906

    Purpose

    ePiggyBac mammalian expression plasmid for CymR-GFP fusion

    Depositor

    Orion Weiner

    Article

    Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

    Insert

    CymRV5-nls-GFP
    

    Expression

    Mammalian

    Available Since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pROSA26-CreERT2

    Plasmid

    #149437

    Purpose

    ROSA26 targetting vector with tamoxifen-inducible Cre recombinase

    Depositor

    Primo Schaer

    Article

    Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

    Insert

    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    ERT2

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CRY2-GFP-homer1c

    Plasmid

    #89442

    Purpose

    Localizes CRY2 to excitatory synapses

    Depositor

    Matthew Kennedy

    Article

    Sinnen et al Neuron. 2017 Feb 8;93(3):646-660.e5. doi

    Insert

    CRY2-GFP-homer1c
    

    Expression

    Mammalian

    Mutation

    Contains Full length CRY2

    Promoter

    CAG

    Available Since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRF-TetR

    Plasmid

    #49374

    Depositor

    Christopher Voigt

    Article

    Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

    Insert

    TetR repressor
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLV CMV FST-FLAG

    Plasmid

    #175167

    Purpose

    Lentiviral expression of human FST-FLAG

    Depositor

    Larry Gerace

    Insert

    FST (FST Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mCh-KIF5B*-strep

    Plasmid

    #120164

    Purpose

    Expresses a chimera of mCherry (fluorescent tag), a KIF5B-based anterograde motor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member 5B (Kif5b Mouse)
    

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated KIF5B: 1-555 aa

    Promoter

    CMV

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDONR221_SLC6A15

    Plasmid

    #131877

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC6A15 (SLC6A15 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 9, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. qTAG-N-Puro-mStayGold-ACTB

    Plasmid

    #227320

    Purpose

    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    ACTB Homology Arms flanking a Puro-2A-mStayGold Cassette (ACTB Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge