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pD649-HAsp-mCherry-Fc(DAPA)-AviTag-6xHis

Plasmid

#156581

Purpose

Mammalian expression of mCherry fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

mCherry

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pHDM-Tat1b

Plasmid

#164442

Purpose

Encodes HIV-1 Tat accessory protein for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

HIV-1 Tat

Expression

Mammalian

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pRDB_347

Plasmid

#231114

Purpose

CBE activity-based selection cloning vector

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FluoSTEP-ICUE

Plasmid

#181845

Purpose

Genetically encoded FRET-based sensor for monitoring cAMP dynamics near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

FluoSTEP-ICUE (RAPGEF3 Human)

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5F Smad2

Plasmid

#12623

Depositor

Rik Derynck

Article

Zhang et al Nature. 1998 Aug 27. 394(6696):909-13.

Insert

Smad2 (SMAD2 Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsDCP2-E148Q_U

Plasmid

#147643

Purpose

Mammalian Expression of HsDCP2-E148Q

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsDCP2-E148Q (DCP2 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-sfGFP

Plasmid

#199171

Purpose

used to express a superfolder GFP in E. coli for comparison to supercharged variants

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

superfolder GFP

Tags

MGHHHHHHGGA

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR v2-sgControl

Plasmid

#230080

Purpose

Crispr knock out control

Depositor

Xingguo Zhu

Article

Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

Insert

non-specific sequence control

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pMN-334

Plasmid

#195468

Purpose

Lentiviral Integratable Inducible synZiFTR ZF10 + Reporter

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

inverted[8X ZF10 BS - ybTATA - mCherry] - pSFFV - ZF10 - NS3 - p65

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLY093-EFS-BCMA-Blast-WPRE

Plasmid

#192194

Purpose

Lenti-BCMA-OE-Blast

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Lenti-BCMA-OE-Blast (TNFRSF17 Synthetic)

Use

Lentiviral; Mammalian expression

Mutation

NA

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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MF82_PB-TRE3G-12x42bs-10x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA

Plasmid

#176626

Purpose

TF A self-activation cassette in MultiFate-2.2 and MultiFate-3

Depositor

Michael Elowitz

Article

Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

Insert

TRE3G-12x42bs-10x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA

Use

Synthetic Biology

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5 TGF beta type I receptor (T202D) Flag

Plasmid

#14833

Depositor

Rik Derynck

Article

Feng et al J Biol Chem. 1996 May 31. 271(22):13123-

Insert

TGF beta type I receptor (Tgfbr1 Rat)

Tags

flag

Expression

Mammalian

Mutation

Threonine 202 mutated to aspartate. Constitutivel…

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

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pMK262 (RAD21-mAC Neo)

Plasmid

#140538

Purpose

RAD21 tagging with mAID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

RAD21 (RAD21 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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HA-Elongin B-pcDNA3.1(+)-Zeo

Plasmid

#20000

Depositor

William Kaelin

Article

Ohh et al Nat Cell Biol. 2000 Jul . 2(7):423-7.

Insert

transcription elongation factor B (ELOB Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wild type

Available Since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

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TFORF2031

Plasmid

#142906

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

PRDM1 (PRDM1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pNC-Cam2304-RNAi

Plasmid

#193400

Purpose

Nimble cloning-compatible vector for plant RNAi

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Expression

Plant

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Myc-ASC

Plasmid

#73952

Purpose

Express c-Myc-tagged human ASC in mammalian cells

Depositor

Christian Stehlik

Article

Bryan et al J Immunol. 2009 Mar 1;182(5):3173-82. do

Insert

PYD and CARD domain containing, PYCARD (PYCARD Human)

Tags

c-myc

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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HA-ERalpha

Plasmid

#215093

Purpose

Expresses HA-ERalpha in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

ESR1 (ESR1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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Tcf/Lef:H2B-GFP

Plasmid

#32610

Depositor

Anna-Katerina Hadjantonakis

Article

Ferrer-Vaquer et al BMC Dev Biol. 2010 Dec 21;10:121.

Insert

Tcf/Lef:H2B-GFP

Promoter

hsp68

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-REL

Plasmid

#27253

Depositor

Thomas Gilmore

Article

Leeman et al Oncogene. 2008 Dec 4. 27(53):6770-81.

Insert

REL (REL Human)

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 1, 2011

Availability

Academic Institutions and Nonprofits only

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pET28b-ULP1-405-621

Plasmid

#229224

Purpose

For expression of ULP1 protein

Depositor

Andy LiWang

Insert

ULP1 (ULP1 Budding Yeast)

Expression

Bacterial

Mutation

405-621 (truncated)

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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SpRY-PCE(cm24Cre)

Plasmid

#242426

Purpose

for chromosome editing

Depositor

Caixia Gao

Article

Sun et al Cell. 2025 Aug 1:S0092-8674(25)00800-1.

Insert

SpRY-PE, cm24Cre

Use

CRISPR

Expression

Plant

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Hs.cargo(Cas9)

Plasmid

#174864

Purpose

Expresses human Peg10 cargoRNA encoding SpCas9

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

SpCas9 flanked by HsPeg10 UTRs

Tags

HA-SV40 NLS and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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CCR5_gRNA_pX459

Plasmid

#192268

Purpose

gRNA expression for CRISPR Genome Editing of CCR5

Depositor

Shantanu Chowdhury

Article

Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

Insert

CCR5 guide RNA

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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ADRA2B-Tango

Plasmid

#66217

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

ADRA2B (ADRA2B Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pBAD-Citroff1

Plasmid

#134301

Purpose

Bacterial expression of inverse-response citrate sensor with high binding affinity

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citroff1

Tags

6-His tag

Expression

Bacterial

Promoter

pBAD

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pFBOH-LIC_DDB1:1-1140

Plasmid

#210886

Purpose

Insect expression of full-length human DDB1. Untagged.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DDB1:M1-H1140 (DDB1 Human)

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pWZL Neo Myr Flag LIMK2

Plasmid

#20513

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

LIMK2 (LIMK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pCAX APPs-695-alpha

Plasmid

#30147

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

Amyloid Precursor Protein - soluble (APP Human)

Expression

Mammalian

Mutation

amino acids 1–612

Available Since

Aug. 15, 2011

Availability

Academic Institutions and Nonprofits only
pLVX-hNINJ1-GFP

Plasmid

#208779

Purpose

Inducibly expresses human NINJ1 tagged with GFP

Depositor

Petr Broz

Article

Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

Insert

NINJ1 (NINJ1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Aug. 2, 2024

Availability

Academic Institutions and Nonprofits only

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