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pALOD4
Plasmid#111026PurposeExpresses domain 4 of anthrolysin O cholesterol sensor, has N-terminal His6-tag and two point mutations, S404C and C472ADepositorInsertDomain 4 of Anthrolysin O
UseTagsHis6 tagExpressionBacterialMutationMutations: S404C, C472APromoterT7Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PBD-EYFP
Plasmid#13723DepositorInsertPBD-EYFP (PAK1 Human)
UseTagsEYFPExpressionMammalianMutationp21 binding domain of human Pak1(amino acids 65-1…PromoterAvailable sinceMarch 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pACYCT2
Plasmid#45799DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
AIO-mCherry
Plasmid#74120PurposeAll-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editingDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
AXBEv2
Plasmid#204606PurposeMammalian expression of evolved adenine transversion base editor AXBEv2DepositorInsertbpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP
UseCRISPRTags6*HisExpressionMammalianMutationmAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)PromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
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WT A1-LCD +NLS
Plasmid#178658PurposeBacterial expression of N-terminally 6His tagged WT A1-LCD (LCD: low-complexity domain, aa 186-320)DepositorInsertWT A1-LCD +NLS (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Hygro DEST ERKKTRmCerulean3
Plasmid#90229PurposeLentiviral vector to express ERK KTR mCerulean3 under PGK promoter (With Hygromycin Resistance)DepositorInsertERK Kinase Translocation Reporter
UseLentiviralTagsmCerulean3ExpressionMammalianMutationPromoterPGKAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pQCXIZ-turq-3xFLAG-TREX1 D18N
Plasmid#164233Purposeretroviral plasmid for turq-3xFLAG-TREX1 D18N (catalytically-deficient)DepositorInsertTREX1 (TREX1 Human)
UseRetroviralTagsFLAG and mturquoiseExpressionMammalianMutationD18N ->nuclease deficientPromoterCMVAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
MYC_pLX307
Plasmid#98363PurposeLentiviral expression of MYCDepositorInsertMYC (MYC Human)
UseLentiviralTagsNAExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT3EF1aH-myr-Akt
Plasmid#179909PurposeThis plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.DepositorInsertAkt1 (Akt1 Mouse)
UseTagsHA and MyrExpressionMammalianMutationPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hPTGS2-2flag
Plasmid#102498PurposeMammalian expression of PTGS2DepositorInsertPTGS2 (PTGS2 Human)
UseTagsFlag-FlagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15-UbE2D2
Plasmid#15783DepositorInsertUbiquitin conjugating enzyme UbE2D2 (UBE2D2 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHR-DDX4N-mCh-Cry2WT
Plasmid#101225PurposeDDX4(1-236) fused to mCh-Cry2WTDepositorInsertDDX4(1-236)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceFeb. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSP574
Plasmid#98807PurposeExpression VectorDepositorInsertpET28 MBP-ENLYFQS-GST
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSEVA221pmCDA1-T7RNAP
Plasmid#167978PurposeExpresses fusion protein pmCDA1-T7RNAP in E. coliDepositorInsertpmCDA1-T7RNAP (T7p07 Synthetic)
UseTagsT7 tag and T7RNAPExpressionBacterialMutationPromoterTetR-PtetAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE52
Plasmid#90397PurposeSTAT1 - STAT1 gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterSTAT1Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
GAPDH-CPG-TV
Plasmid#70148Purposea targeting vector, to target the endogenous Gapdh CPG island with Snrp-GFP-polyADepositorInsert5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh
UseMouse Targeting and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL410_Ins2500
Plasmid#49058PurposeExpresses luciferase under control of mouse insulin 2 gene promoterDepositorInsertmouse Ins2 promoter (Ins2 Mouse)
UseTagsExpressionMammalianMutationPromoterIns2 promoterAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 CCNE1
Plasmid#164144PurposeDoxycycline inducible expression of Cyclin E1DepositorInsertCCNE1 (CCNE1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCherryGFP-CoV2-WT
Plasmid#177618PurposeDual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshiftingDepositorInsertSARS-CoV-2 FSE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pinducer 20 DN-KASHΔPPPL
Plasmid#129280PurposeTet-ON inducible lentivirus expressing mcherry-labeled DN KASHΔPPPLDepositorInsertSYNE1 (SYNE1 Human)
UseLentiviralTagsmcherryExpressionMutationthe last 4 amino acids (PPPL) of the KASH domain …PromoterCMVAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEW100v5-177-HYG
Plasmid#24013DepositorInsertLuciferase
UseTrypanosome expressionTagsExpressionMutationK520RPromoterAvailable sinceFeb. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEx-4T-1-YTHDF3
Plasmid#70088PurposeEncode YTHDF3 isoform a with N-terminal GST tag for E. coli expressionDepositorInsertYTH N(6)-methyladenosine RNA binding protein 3 (YTHDF3 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGolt-mCherry / pGolt3-mCherry
Plasmid#73297PurposeVisualization of Dendritic Golgi SatelliteDepositorInsertsER export signal of Scap
ER export signal of Scap
TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)
UseTagsER export and mCherryExpressionMammalianMutationPromoterCMV immediate early promoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv1(mCherry-FLAG) in pTwist-CMV
Plasmid#216165PurposeExpression of mCherry specifically in cells with TDP-43 knockdown. Uses AARS1-derived cryptic exon to control mCherry expression. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor CommentsDepositorInsertmCherry with C-terminal FLAG tag
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET His6 NusA TEV LIC cloning vector (1N)
Plasmid#29657DepositorTypeEmpty backboneUseTags6xHis, NusA, and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only
