We narrowed to 19,089 results for: iNOS

1. mGFP-hNET

   Plasmid

   #193364

   Purpose

   expression of human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cells

   Depositor

   Harald Sitte

   Article

   Luethi et al Commun Biol. 2022 Nov 17;5(1):1259. doi:

   Insert

   human Norepinephrine Transporter (SLC6A2 Human)
   

   Use

   Tags

   monomeric GFP (mGFP)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS D614G soluble Spike

   Plasmid

   #164651

   Purpose

   Expresses SARS-CoV-2 soluble Spike protein with a D614G mutation

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   SARS-CoV-2 soluble spike (S SARS-CoV-2)
   

   Use

   Tags

   HIS

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   CAG

   Available Since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

3. pCW57.1 KBTBD4 WT

   Plasmid

   #184624

   Purpose

   Doxycycline inducible lentiviral vector for N-terminal Flag tagged KBTBD4 WT expression in mammalian cells

   Depositor

   Vincenzo D'Angiolella

   Article

   Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

   Insert

   N-terminal Flag tagged KBTBD4 WT (KBTBD4 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   no mutation

   Promoter

   TRE promoter, Tet ON

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Ef1a-Con/Foff 2.0-GCaMP6M

   Plasmid

   #137120

   Purpose

   Intersectional viral expression of GCaMP6M in cells expressing Cre AND NOT Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Foff 2.0-GCaMP6M
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   Removed RSET tag

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223_YY1_WT

   Plasmid

   #82171

   Purpose

   Gateway Donor vector containing YY1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   YY1 (YY1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT3-EFa1-HA-Jag1

   Plasmid

   #46051

   Depositor

   Xin Chen

   Article

   Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

   Insert

   Jag1 (Jag1 Rat)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-nEF-Coff/Fon-NpHR3.3-EYFP

   Plasmid

   #137154

   Purpose

   Intersectional viral expression of NpHR3.3-EYFP in cells expressing Flp AND NOT Cre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-NpHR3.3-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   W179F

   Promoter

   nEF

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOT_4 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-LTBR

   Plasmid

   #181973

   Purpose

   Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

   Insert

   FMC6.3-BBz CAR; LTBR (LTBR Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pscALPSpuro-SsACE2 (Pig)

   Plasmid

   #158085

   Purpose

   Expresses pig ACE2 in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   Pig ACE2 (ACE2 Sus scrofa)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP

    Plasmid

    #137148

    Purpose

    Intersectional viral expression of Arch3.3-EYFP in cells expressing both Cre AND Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-Arch3.3-p2a-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    nEF

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-nEF-Con/Fon-ChR2-mCherry

    Plasmid

    #137142

    Purpose

    Intersectional viral expression of ChR2-mCherry in cells expressing both Cre AND Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-ChR2-mCherry
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    H134R

    Promoter

    nEF

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-nEF-Con/Foff 2.0-ChR2-EYFP

    Plasmid

    #137163

    Purpose

    Intersectional viral expression of ChR2-EYFP in cells expressing Cre AND NOT Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-ChR2-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    H134R

    Promoter

    nEF

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEMS2230

    Plasmid

    #158420

    Purpose

    AAV plasmid with Ple253 (PITX3 MiniPromoter) driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple253 (PITX3 )
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ple253

    Available Since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-EFS-BirAopt-GSQ-RNF4ca-P2A-BLAST

    Plasmid

    #208047

    Purpose

    Enables constitutive expression of N-terminal BirAopt-fused RNF4ca (catalytically inactive RING mutant); to perform bioE3; selection with blasticidin

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    RNF4 (RNF4 Human)
    

    Use

    Lentiviral

    Tags

    BirAopt

    Expression

    Mutation

    Cys>Ala mutations in conserved RING domain

    Promoter

    EFS

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. alpha actinin delta ABD-GFP

    Plasmid

    #66935

    Purpose

    Mammalian expression of alpha actinin with actin binding domain (amino acids 30–253) deleted

    Depositor

    Pere Roca-Cusachs, Michael Sheetz

    Article

    Roca-Cusachs et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

    Insert

    Alpha-actinin 1 (ACTN1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    actin binding domain (amino acids 30–253) is dele…

    Promoter

    CMV

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-nEF-Coff/Fon-ChR2-mCherry

    Plasmid

    #137144

    Purpose

    Intersectional viral expression of ChR2-mCherry in cells expressing Flp AND NOT Cre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-ChR2-mCherry
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    H134R

    Promoter

    nEF

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pscALPSpuro-ClACE2 (Dog)

    Plasmid

    #158083

    Purpose

    Expresses dog ACE2 in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    Dog ACE2 (ACE2 )
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-2xFLAG-2xSTREP-CCND1

    Plasmid

    #172648

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged cyclin D1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOT_3 - lenti-EFS-FMC6.3-28z-2A-puro-2A-LTBR

    Plasmid

    #181972

    Purpose

    Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    FMC6.3-28z CAR; LTBR (LTBR Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC178: pAAV.U6.SapI.CMV.Cas13bt3

    Plasmid

    #204215

    Purpose

    Plasmid AAV vector expressing Cas13bt3 and hU6-driven expression of guide RNAs. Contains SapI sites for guide cloning flanked by 5' and 3' full-length DRs.

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Insert

    Cas13bt3 and U6.SapI sgRNA cloning site
    

    Use

    AAV and CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Codon optimisation by GenScript tool

    Promoter

    CMV/U6

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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