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pGADT7-GW

Plasmid

#61702

Purpose

Gateway compatible Y2H vector - AD

Depositor

Yuhai Cui

Article

Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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PCDH-WT-ALKBH5-3xFLAG

Plasmid

#203736

Purpose

Overexpression of ALKBH5-WT

Depositor

Jianjun Chen

Article

Shen et al Cell Stem Cell. 2020 Jul 2;27(1):64-80.e

Insert

ALKBH5 (ALKBH5 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-cmyc

Plasmid

#16011

Purpose

mammalian expression of human cmyc

Depositor

Wafik El-Deiry

Article

Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

Insert

cmyc (MYC Human)

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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His-Tev-UbE1

Plasmid

#186804

Purpose

Codon-optimized bacterial expression plasmid. Expresses ubiquitin activating enzyme His-Tev-UbE1.

Depositor

Lalit Deshmukh

Article

Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

Insert

His-Tev-UbE1 (UBA1 Human)

Tags

His6-Tev

Expression

Bacterial

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBiFCt-2in1-CC

Plasmid

#105114

Purpose

ratiometric BiFC analysis in plants (transient transformation)

Depositor

Christopher Grefen

Article

Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

Type

Empty backbone

Tags

cYFP-Myc/nYFP-HA

Expression

Plant

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-Cre-iBSD

Plasmid

#140284

Purpose

Expression of Cre-IRES-BSD

Depositor

Yusuke Miyanari

Article

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

Cre

Expression

Mammalian

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS-GFP(ATG mut)

Plasmid

#197885

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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19ag-SOD2-3xHA-TALE(RNR2-Left)-MutH

Plasmid

#209294

Purpose

mitoBE expression vector, target mtRNR2 gene.

Depositor

Wensheng Wei

Article

Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

Insert

SOD2-3xHA-TALE(RNR2-Left)-MutH

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pARSCL504

Plasmid

#198636

Purpose

Expresses a transgene encoding RUBY

Depositor

Shaun Curtin

Insert

35S:Ω:Ruby

Expression

Plant

Available Since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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pGLO-GFP-1UAG

Plasmid

#82500

Purpose

Expresses GFP with 1 UAG codon at the amino acid position 3

Depositor

Chang Liu

Article

Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

Insert

Green Fluorescent Protein with 1 UAG codon

Expression

Bacterial

Mutation

Added an UAG codon at the amino acid position 3

Promoter

AraBAD

Available Since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-Cre

Plasmid

#196140

Purpose

AAV-mediated expression of Cre under the CAG promoter .

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Cre

Use

AAV

Expression

Mammalian

Mutation

codon diversified

Promoter

CAG

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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CTCF-mAC donor (Neo)

Plasmid

#140645

Purpose

CTCF tagging with mAID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

CTCF (CTCF Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCHC005 (∆RE)

Plasmid

#226199

Purpose

Conjugation knockout plasmid (pK18mobsacB) to delete H16_0006 (Type I restriction endonuclease subunit).

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for type I restriction-modification system restriction subunit H16_A0006

Use

Synthetic Biology

Available Since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pET30-R5-GFP

Plasmid

#17838

Depositor

Jay Keasling

Article

Shaikh et al Anal Chem. 2008 Feb 1. 80(3):886-90.

Insert

pET30-GFP

Use

Synthetic Biology

Tags

His, R5, and S-tag

Expression

Bacterial

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only

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pAM5505

Plasmid

#132664

Purpose

pRL623-based helper plasmid for conjugations, with RSF1010 mobA gene

Depositor

Susan Golden

Article

Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

Insert

MobA

Expression

Bacterial

Mutation

please see depositor comments below

Promoter

p1,p3

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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CT752

Plasmid

#233658

Purpose

Flp GFP Reporter; FRT-STOP-FRT-GFP

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

FRT-STOP-FRT-GFP

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pNC-Cam1304-RNAi

Plasmid

#193399

Purpose

Nimble cloning-compatible vector for plant RNAi

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Expression

Plant

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Plasmid L5 attB::Pleft* mCherry

Plasmid

#169411

Purpose

Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mCherry

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

pLeft
mCherry

Use

Unspecified

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCONR221

Plasmid

#216146

Purpose

Gateway Donor Vector with attP1 and attP2 sites modified from PDONR221 and Ampicillin resistance

Depositor

Imran Siddiqi

Article

Sivakumar et al Physiol Mol Biol Plants. 2024 Jul;30(7):

Type

Empty backbone

Use

Cloning backbone

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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p276 eSpCas9_2gRNAs_hH11

Plasmid

#164850

Purpose

gRNA vector for targeting human H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Tissue Eng Regen Med. 2020 Apr;17(2):223

Insert

gRNAs for targeting human H11 locus

Expression

Mammalian

Promoter

U6

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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