We narrowed to 10,337 results for: plasmids 101

1. pBK1309-AAV-EFSNC-dSaCas9-MBD1(529-592)

   Plasmid

   #223154

   Purpose

   Expression of truncated MBD1 with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   MBD1 (MBD1 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 529-592

   Promoter

   EF1a

   Available Since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBK1310-AAV-EFSNC-dSaCas9-MBD2(176-231)

   Plasmid

   #223155

   Purpose

   Expression of truncated MBD2 with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   MBD2 (MBD2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 176-231

   Promoter

   EF1a

   Available Since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. B2M-SDMutation-EhT

   Plasmid

   #215546

   Purpose

   Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Insert

   B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived hygromycin-NLS-tdTomato selection cassette (B2M Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   The second base of the first intron of B2M (From …

   Promoter

   Available Since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. B2M-SDMutation-EPG

   Plasmid

   #215545

   Purpose

   Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Insert

   B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived puromycin-GFP selection cassette (B2M Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   The second base of the first intron of B2M (From …

   Promoter

   Available Since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV6-AN-DDK_S172A_M173A_V195A RAD23A

   Plasmid

   #201576

   Purpose

   Expresses a variant of human RAD23A containing mutations S172A, M173A and V195A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains mutations S172A, M173A and V195A

   Promoter

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Coexpressed Free NES

   Plasmid

   #182482

   Purpose

   Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and nerolidol synthase from Actinidia chinensis (AcNES1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

   Inserts

   FPPS
   NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL10 and GAL7

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. PWPXLD-EGFR-P667A

   Plasmid

   #133750

   Purpose

   EGFR with mutation at proline 667 converted to alanine and tagged with HA cloned into PWPXLD plasmid

   Depositor

   Chay Kuo

   Article

   Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4

   Insert

   Mutant P667A EGFR-HA (EGFR Human)
   

   Use

   Lentiviral

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   proline 667 converted to alanine (Please see depo…

   Promoter

   EF1-alpha

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONOR-SNCAe2-WT

   Plasmid

   #85845

   Purpose

   Donor plasmid for SNCA exon2 wild type sequence. Also contains TagBFP and EGFP

   Depositor

   Jens Schwamborn

   Article

   Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

   Insert

   SNCA exon 2 homology arms (SNCA Human)
   

   Use

   Tags

   -

   Expression

   Bacterial and Mammalian

   Mutation

   -

   Promoter

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPRIME-CMV-GFP-shCrh2

   Plasmid

   #132710

   Purpose

   Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Crh gene

   Depositor

   Robert Messing

   Article

   Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

   Insert

   shCrh(2) (Crh Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKLV2-U6gRNA5(Apc-g1)-PGKpuroBFP-W

    Plasmid

    #105022

    Purpose

    Lentiviral gRNA plasmid targeting mouse Apc , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Apc (Apc Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLL5 sgRNA(MS2)-MS2-hA3a D131A

    Plasmid

    #112131

    Purpose

    empty sgRNA cloning vector with MS2-humanA3a D131A

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    human Apobec3a D131A mutant (APOBEC3A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D131A mutant in hA3a

    Promoter

    hU6,CBh

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONOR-SNCAe3-A53T

    Plasmid

    #85848

    Purpose

    Donor plasmid for SNCA exon3 A53T sequence. Also contains EGFP and tagBFP

    Depositor

    Jens Schwamborn

    Article

    Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

    Insert

    SNCA exon 3 homology arms (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLentiCMV Puro DEST p38KTRmCerulean3

    Plasmid

    #59155

    Purpose

    Lentiviral vector to express p38 KTR mCerulean3 under CMV promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    p38 Kinase Translocation Reporter (MAPK14 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-DIO {mCAR}off-{GCaMP6f}on-WPRE

    Plasmid

    #111393

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON GCaMP6f (ultrasensitive calcium sensor)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    mCAR (Cxadr Mouse)
    GCaMP6f
    

    Use

    AAV

    Tags

    6xHis, Myc, T7, and Xpress

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-DIO {mCAR}off-{DTR-GFP}on-WPRE

    Plasmid

    #111395

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection), Cre-ON DTR-EGFP (diphtheria toxin receptor for cell ablation)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    mCAR (Cxadr Mouse)
    DTR-EGFP
    

    Use

    AAV

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. FUW-tetO-hOKMS

    Plasmid

    #51543

    Purpose

    Inducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2

    Depositor

    Tarjei Mikkelsen

    Article

    Cacchiarelli et al Cell. 2015 Jul 16;162(2):412-24. doi: 10

    Insert

    hOKMS (MYC, KLF4, SOX2, POU5F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV_BiPVe3_Gq_dTomato

    Plasmid

    #213942

    Purpose

    AAV construct expressing HA-hM3Dq-dTomato driven by PV+ basket cell-targeting enhancer. Alias: pAAV_WDP0003_Gq_dTomato

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Insert

    HA-GqDREADD-dTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV_BiPVe4_Gq_dTomato

    Plasmid

    #213938

    Purpose

    AAV construct expressing HA-hM3Dq-dTomato driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_Gq_dTomato

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Insert

    HA-GqDREADD-dTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV_BiSSTe4_Gq_dTomato

    Plasmid

    #213946

    Purpose

    AAV construct expressing HA-hM3Dq-dTomato driven by SST interneuron-targeting enhancer. Alias: pAAV_WDS0004_Gq_dTomato

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Insert

    HA-GqDREADD-dTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV_BiVIPe4_Gq_dTomato

    Plasmid

    #213858

    Purpose

    AAV construct expressing HA-hM3Dq-dTomato driven by VIP interneuron-targeting enhancer. Alias: pAAV_YWE4.2_Gq_dTomato

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Insert

    HA-GqDREADD-dTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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