We narrowed to 9,381 results for: BLI

1. pPyCAG-mKate2-3xNLS-IRES-Pac

   Plasmid

   #183618

   Purpose

   Mammalian expression vector for mKate2-3xNLS from the CAG promoter. Confers puromycin resistance.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Elife. 2013 Dec 17;2:e01197. doi: 10.755

   Insert

   mKate2-3xNLS
   

   Tags

   3xNLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJ151-HDR

   Plasmid

   #113631

   Purpose

   Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.

   Depositor

   Alex Kentsis

   Inserts

   Left homology arm
   Puromycin resistance gene
   Venus fluorescence gene
   Thymidine kinase
   Right homology arm
   

   Use

   Cre/Lox; Homology recombination selection vector

   Expression

   Bacterial and Mammalian

   Promoter

   EF1a promoter, EF1a promoter (T2A cleaving sequen…

   Available Since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. AICSDP-57: SMC1A-mEGFP

   Plasmid

   #114406

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human SMC1A

   Depositor

   Allen Institute for Cell Science

   Insert

   SMC1A Homology Arms with linker-mEGFP (SMC1A Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   Sept. 4, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pLenti Puro HA-Ubiquitin-K48

   Plasmid

   #236290

   Purpose

   Lentiviral vector expressing HA-tagged ubiquitin K48 mutant

   Depositor

   Melina Fan, Addgene Research Program

   Insert

   Ubiquitin K48 (UBB Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   Single ubiquitin coding sequence containing K48 m…

   Promoter

   CMV

   Available Since

   April 10, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pLenti Puro HA-Ubiquitin-K63

   Plasmid

   #236291

   Purpose

   Lentiviral vector expressing HA-tagged ubiquitin K63 mutant

   Depositor

   Melina Fan, Addgene Research Program

   Insert

   Ubiquitin K63 (UBB Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   Single ubiquitin coding sequence containing K63 m…

   Promoter

   CMV

   Available Since

   April 10, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pAAV/hSyn-SCLM

   Plasmid

   #216760

   Purpose

   AAV2 transfer vector with the human synapsin I promoter for neuronal expression of SuperClomeleon

   Depositor

   George Augustine

   Insert

   SuperClomeleon
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   S30R and 11 AA deletion in the Cerulean CFP moiet…

   Promoter

   hSyn (human synapsin I)

   Available Since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-osTIR1-9xMyc-Zeo

   Plasmid

   #225696

   Purpose

   Lentiviral expression of 9xMycTag fused osTIR1and Zeocin resistance. For use with AID degron.

   Depositor

   Lacra Bintu

   Article

   Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

   Insert

   osTIR1
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   9X Myc

   Expression

   Mammalian

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKOR1-mcs

   Plasmid

   #181755

   Purpose

   Allele replacement for Staphylococcus aureus

   Depositor

   Suzan Rooijakkers

   Article

   Stapels et al Proc Natl Acad Sci U S A. 2014 Sep 9;111

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEMTEZ-Kir2.1

   Plasmid

   #32641

   Depositor

   Richard Axel, Joseph Gogos, C. Ron Yu

   Article

   Yu et al Neuron. 2004 May 27;42(4):553-66.

   Insert

   Kir2.1 (Kcnj2 Mouse)
   

   Expression

   Bacterial

   Available Since

   Aug. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQTEV-STMN1

    Plasmid

    #31326

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    stathmin 1/oncoprotein 18 (STMN1 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. ITCH

    Plasmid

    #36197

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ITCH (ITCH Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    contains HECT domain

    Available Since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

12. 2xMARS-nGFP

    Plasmid

    #205235

    Purpose

    Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells

    Depositor

    Jeremy Baskin

    Insert

    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    

    Tags

    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDB179-LCC

    Plasmid

    #218686

    Purpose

    Encodes codon optimized LCC enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    LCC
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-PGK-CXCR4 (neo)

    Plasmid

    #192077

    Purpose

    Lentivirus for expression of CXCR4

    Depositor

    Roland Friedel

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    Lentiviral

    Promoter

    hPGK

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOTTC1761 - pAAV SYN1 DIO HA-hM3D(Gq)

    Plasmid

    #125147

    Purpose

    An adeno-associated viral vector expressing Cre-dependent HA-tagged inhibitory DREADD (hM3D(Gq)) from a synapsin promoter

    Depositor

    Christopher Richie

    Insert

    HA-tagged hM3D(Gq) (CHRM3 Human)
    

    Use

    AAV and Cre/Lox

    Tags

    HA

    Expression

    Mammalian

    Promoter

    SYN1

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-sCAG-eGFP-RAB11

    Plasmid

    #203799

    Purpose

    AAV vector plasmid expressing human RAB11 fused to eGFP under a short variant of the CAG (sCAG) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Insert

    RAB11 (RAB11A Human)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLTR-SFFV-PTPN2 transcript variant 1-WPRE-UbC-Emerald

    Plasmid

    #201186

    Purpose

    Lentiviral vector plasmid expressing human PTPN2 transcript variant 1 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis

    Inserts

    PTPN2, transcript variant 1 (PTPN2 Human)
    Emerald
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pdCas9-NED

    Plasmid

    #109358

    Purpose

    Mammalian vector for expressing effector domains fused to dCas9.

    Depositor

    Antal Kiss

    Article

    Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    dCas9, SV40 NLS, 3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFP.R267

    Plasmid

    #138179

    Purpose

    Expresses the split mCherry reporter interrupted by the gp41-1 intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(SGY).gp41-1.(SSS)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    P(araBAD) and iPC

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. mApple-hCOL1A1

    Plasmid

    #140113

    Purpose

    Expresses fluorescently-tagged human Type I procollagen α1 chain with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Insert

    Type I procollagen α1 (COL1A1 Human)
    

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    COL1A1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available Since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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