Addgene

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pAAV.Syn-FLEX.iGABASnFR.F102G.mRuby3

Plasmid

#112170

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Use

AAV

Tags

mRuby3

Expression

Mutation

F102G

Promoter

Synapsin

Available since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/HisC 14-3-3 epsilon wt

Plasmid

#11942

Depositor

Michael Yaffe

Article

Brunet et al J Cell Biol. 2002 Mar 4. 156(5):817-28.

Insert

Ywhae (Ywhae Rat)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pAAV.hSynap.iGABASnFR.R205A

Plasmid

#112162

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.R205A

Use

AAV

Tags

Expression

Mutation

R205A (no GABA binding)

Promoter

hSynapsin

Available since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.iGABASnFR.F102Y.Y137L

Plasmid

#112161

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102Y.Y137L

Use

AAV

Tags

Expression

Mutation

F102Y.Y137L

Promoter

hSynapsin

Available since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.iGABASnFR.F102Y.Y137L

Plasmid

#112174

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102Y.Y137L

Use

AAV

Tags

Expression

Mutation

F102Y.Y137L

Promoter

GFAP

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB

Plasmid

#78348

Purpose

Expresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

Depositor

Michael Elowitz

Article

Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

Insert

H2B-mCherry-T2A-rTetR-KRAB

Use

Synthetic Biology

Tags

rTetR

Expression

Mammalian

Mutation

Promoter

pEF alpha

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Ha-Brca1 S1423A/S1524A

Plasmid

#32539

Depositor

Michael Kastan

Article

Xu et al Cancer Res. 2002 Aug 15;62(16):4588-91.

Insert

BRCA1 (BRCA1 Human)

Use

Tags

Expression

Mammalian

Mutation

S1423A/S1524A

Promoter

CMV/rabbit B-Globin

Available since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only
pCDNA4/TO-GFP-SPOP (WT)

Plasmid

#197008

Purpose

Mammalian inducible expression vector for GFP-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

-

Promoter

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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DDX5 D248N V5

Plasmid

#199577

Purpose

Expresses helicase dead DDX5 cloned into pLenti puro

Depositor

Michael Kastan

Article

McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

Insert

DDX5 (DDX5 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

D248N

Promoter

CMV

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSW002-PpsbA-mNeonGreen

Plasmid

#205017

Purpose

Broad host-range bacterial expression vector with constitutive PsbA promoter; mNeonGreen (codon optimized for P. fluorescens)

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

mNeonGreen

Use

Tags

Expression

Bacterial

Mutation

mNeonGreen is codon optimized for expression in P…

Promoter

PpsbA

Available since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.iGABASnFR.F102G

Plasmid

#112167

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Use

AAV

Tags

Expression

Mutation

F102G

Promoter

CAG

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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PXPR007 sgGFP-2

Plasmid

#74961

Purpose

Cas9 + sgGFP-2 with blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

GFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-WRAP53beta

Plasmid

#64676

Purpose

expresses Wild type WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

cmv

Available since

May 22, 2015

Availability

Academic Institutions and Nonprofits only
pAAV.CAG-FLEX.iGABASnFR.F102Y.Y137L

Plasmid

#112168

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102Y.Y137L

Use

AAV

Tags

Expression

Mutation

F102Y.Y137L

Promoter

CAG

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pSFG-TFII-I-GFP delta

Plasmid

#22190

Depositor

Ananda Roy

Article

Desgranges et al Mol Cell Biol. 2005 Dec . 25(24):10940-5

Insert

TFII-I (GTF2I Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pFH2.102_PB-EPN11-MCP_I-T2A-eGFP-PGK-Blast

Plasmid

#205547

Purpose

Express EPN11-MCP-I-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN11-MCP-I

Use

Piggybac

Tags

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.103_PB-EPN11-MCP_p6-T2A-eGFP-PGK-Blast

Plasmid

#205548

Purpose

Express EPN11-MCP-p6-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN11-MCP-p6

Use

Piggybac

Tags

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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1352wUV2 F2bbs kan GCANNNAGGk

Plasmid

#18752

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

Insert

3 tandem zinc fingers

Use

Tags

Expression

Bacterial

Mutation

A Kan cassette (1090bp) is inserted between two b…

Promoter

Available since

July 11, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/HisC 14-3-3 epsilon K49E

Plasmid

#11943

Depositor

Michael Yaffe

Article

Brunet et al J Cell Biol. 2002 Mar 4. 156(5):817-28.

Insert

Ywhae (Ywhae Rat)

Use

Tags

His

Expression

Mammalian

Mutation

K49E

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
PXNGW

Plasmid

#48258

Purpose

SPLIT-GFP assay in plants

Depositor

Wolf Frommer

Article

Kim et al Plant Cell. 2009 Apr;21(4):1305-23. doi:

Type

Empty backbone

Use

Binary gateway vector

Tags

5xHIS and YFP aa1-172

Expression

Mutation

Promoter

35S

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only