We narrowed to 13,614 results for: sequence
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Plasmid#60335PurposeContains a promoter of activating sequence derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…ExpressionYeastAvailable SinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHBS1412 GFP-TDP43 FYW-L
Plasmid#118801PurposeTo test the effect of sequence on nuclear body formation and splicing of full-length TDP43DepositorInsertTARDBP mutant (TARDBP Human)
UseLentiviralMutationAll FYW to L in CTD of full-length TDP43Available SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3_4xUAS_CP-candidate_luc+
Plasmid#125154PurposeSTAP-seq luciferase validation vector, which harbors an 4x UAS array upstream of the CP position (candidates are inserted at CP position) for the recruitement of GAL4-DBD tagged proteinsDepositorInsert4 x upstream activating sequence (UAS)
UseLuciferaseAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
Or30a-Gal4
Plasmid#63027PurposeTo express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or30a, using the promoter sequences of the gene cloned 5' of Gal4DepositorInsertDrosophila melanogaster Or30a promoter
TagsDrosophila ADH (aa's 1-18) fused to Yeast Ga…ExpressionInsectAvailable SinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOmpW
Plasmid#112121PurposeBacterial expression of outer membrane protein W (OmpW), wild typeDepositorInsertOmpW (ompW E. coli)
ExpressionBacterialMutationN-terminal signal sequence not included and repla…PromoterT7Available SinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hHDAC4
Plasmid#21096DepositorInsertHistone Deacetylase 4 (HDAC4 Human)
ExpressionMammalianMutationshRNA target sequence was mutated (mutated nucleo…PromoterCAGAvailable SinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
phNFL-P440L
Plasmid#132596PurposeExpresses human NFL containing the Charcot-Marie-Tooth disease type 2E P440L mutationDepositorAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
nonphosphorylatable Cenp-B-targted Aurora B FRET sensor
Plasmid#45230DepositorInsertnonphos Cenp-B targeted Aurora B FRET sensor
ExpressionMammalianMutationt-a mutation in substrate sequenceAvailable SinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
PFD1130w-bio-His
Plasmid#50823PurposeExpresses enzymatically monobiotinylated full length PFD1130wDepositorInsertPFD1130w
TagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable SinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMK110
Plasmid#99535Purposecontains bgl operon sequence for E. coli in vitro transcription templateDepositorInsertE. coli bgl antisense region containing bglG
TagsNonePromoterlambda Pr promoter used for in vitro transcriptio…Available SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
gRD21A-mRFP
Plasmid#181959PurposeRD21A protein tagged with mRFP, under control of native promoterDepositorInsertRD21A genomic sequence including 2kb upstream of the ATG
TagsmRFPExpressionPlantPromoterRD21A nativeAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
TAL-L1-1.3-DEL
Plasmid#103035Purpose[Edit] Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by DEL domianDepositorInsertTAL-L1-1.3-DEL
UseT3 in vitro transcription for expression in mamma…TagsDEL and HAAvailable SinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHBS1388 TDP43 RRM-GFP K-R
Plasmid#118796PurposeTo test the effect of sequence on TDP43 RRM-GFP droplet propertiesDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationAll K to R in CTD of TDP43 RRM-GFP reporterAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNP1 tdTomato trigger
Plasmid#107358PurposeTrigger sequence for pNP1 tdTomato toehold switchDepositorInserttoehold trigger 5
ExpressionBacterialPromoterT7Available SinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNP1 Aqua trigger
Plasmid#107360PurposeTrigger sequence for pNP1 Aqua toehold switchDepositorInserttoehold trigger 8
ExpressionBacterialPromoterT7Available SinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZF-BCB-PDZ
Plasmid#127478Purposecoding sequence for synthetic zinc finger ZF-BCB-PDZDepositorInsertZF-BCB synthetic transcription factor fused with the alpha 1-syntrophin PDZ domain
UseCell-free transcription–translationTagsalpha 1-syntrophin PDZ domainPromoterT7Available SinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6A_C
Plasmid#146086PurposeMammalian Expression of HsTNRC6ADepositorInsertHsTNRC6A (TNRC6A Human)
ExpressionMammalianMutationone non silent mutation K1578E compared to the se…Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pET28a-λN-βLac
Plasmid#162039PurposeExpresion of the fusion protein of the λN domain of the λ phage and ampR gene (encoding the enzyme β-lactamase without the native signal sequence)DepositorInsertλN domain of the λ phage and a β-lactamase fusion protein
Tags6xHis tagExpressionBacterialPromoterT7Available SinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTK_Cerulean_Nanotag_66_neg_Control
Plasmid#130567PurposeEnhancer cloning nanotag reporter vectorDepositorInsertnegative oligo sequence
UseEnhancer cloning nanotag reporter vectorPromoterthymidine kinase minimal promoterAvailable SinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only