We narrowed to 17,661 results for: URE

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pCEP4-HA-Flag-natT1R2 a22-end_S212N

Plasmid

#113951

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

delete N-terminal 21 residues, S212N substitution

Promoter

CMV

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAG17 IQGAP1 3'UTR

Plasmid

#14503

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

IQGAP1 3'UTR (IQGAP1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
actc1b-mKate2-EEA1

Plasmid

#109508

Purpose

Muscle specific zebrafish expression of mKate2 fused to early endosome associated 1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

EEA1 (eea1 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-YOD1-MutS2

Plasmid

#85667

Purpose

Expression of human YOD1 I292Q V295Q mutant with N-terminal GFP tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

YOD1 (YOD1 Human)

Tags

GFP

Expression

Mammalian

Mutation

I292Q and V295Q

Promoter

CMV

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST27-GST-ATXN1 FL(2Q)

Plasmid

#21752

Depositor

Huda Zoghbi

Article

Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

Insert

Ataxin-1 (ATXN1 Human)

Tags

GST

Expression

Mammalian

Mutation

insert contains 2Q (2 glutamines) and there isno …

Promoter

CMV

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only
pEBG-MKK4-K129R (GST)

Plasmid

#21564

Depositor

Leonard Zon

Article

Sanchez et al Nature. . 372(6508):794-8.

Insert

mitogen-activated protein kinase kinase 4 (Map2k4 Mouse)

Tags

GST

Expression

Mammalian

Mutation

Lysine 129 to arginine

Available Since

July 17, 2009

Availability

Academic Institutions and Nonprofits only
DNMT1 KO Neo

Plasmid

#16649

Depositor

Bert Vogelstein

Article

Rhee et al Nature. 2002 Apr 4. 416(6880):552-6.

Insert

DNMT1 knock-out construct (DNMT1 Human)

Expression

Bacterial

Available Since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
mKikGR-Tubulin-6

Plasmid

#57330

Purpose

Localization: Microtubules, Excitation: 507 / 583, Emission: 517 / 593

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Tubulin (TUBA1B Human)

Tags

mKikGR

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA3-HA/Bmal L95E

Plasmid

#47344

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Ha

Expression

Mammalian

Mutation

L95E

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
lenti-sgRNA(MS2)_zeo_hPDX1_promoter_guide1

Plasmid

#176838

Purpose

To induce human PDX1 expression by recruiting SAM complex to PDX1 promoter

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

sgPDX1

Expression

Mammalian

Promoter

U6

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_DOCK2-1/1

Plasmid

#91482

Purpose

Protein expression and purification of human SH3 domain construct DOCK2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DOCK2-1/1 (DOCK2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_AMPH-1/1

Plasmid

#91556

Purpose

Protein expression and purification of human SH3 domain construct AMPH-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

AMPH-1/1 (AMPH Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_DBNL-1/1

Plasmid

#91409

Purpose

Protein expression and purification of human SH3 domain construct DBNL-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DBNL-1/1 (DBNL Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGEF16-1/1

Plasmid

#91334

Purpose

Protein expression and purification of human SH3 domain construct ARHGEF16-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGEF16-1/1 (ARHGEF16 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGEF26-1/1

Plasmid

#91342

Purpose

Protein expression and purification of human SH3 domain construct ARHGEF26-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGEF26-1/1 (ARHGEF26 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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HS-DN-Cep152-mKate2

Plasmid

#105943

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

Cep152 (CEP152 Human)

Tags

mKate2

Expression

Bacterial

Mutation

dominant negative (AA 1-512)

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pTAMAHISTEV_Ab-PiuA

Plasmid

#128957

Purpose

expresses Acinetobacter baumannii PiuA in E. coli

Depositor

James Naismith

Article

Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

Insert

TonB-dependent siderophore receptor

Tags

TEV protease cleavable 7xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed

Promoter

T7

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti6.2-coHPDL WT 3xFlag-V5

Plasmid

#174129

Purpose

Expresses codon-optimized HPDL with a 3xFlag-V5 tag in mammalian cells

Depositor

Michael Pacold

Article

Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

Insert

4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)

Use

Lentiviral

Tags

3xFLAG-V5

Expression

Mammalian

Mutation

Codon optimized

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-p53 (1-73) (72R)

Plasmid

#62082

Purpose

Expression of human p53 (1-73) (72R) in e. coli

Depositor

Gary Daughdrill

Article

Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

Insert

p53 (TP53 Human)

Tags

HIs

Expression

Bacterial

Mutation

contains TAD residues 1-73; 72R

Promoter

T7

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
HA-Foxo1-ADA 6KR

Plasmid

#17564

Depositor

Domenico Accili

Article

Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

Insert

Foxo1-ADA 6kr (Foxo1 Mouse)

Tags

HA and Myc

Expression

Mammalian

Mutation

K242, K245, K259, K262, K271 and K291 were replac…

Promoter

CMV

Available Since

Oct. 20, 2008

Availability

Academic Institutions and Nonprofits only
AAV-EF1α1.1-SomArchon-GFP

Plasmid

#153531

Purpose

AAV-mediated expression of SomArchon-GFP under the EF1α1.1 promoter.

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon-GFP

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Promoter

EF1α1.1

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-DysF

Plasmid

#109544

Purpose

Multisite gateway vector for 3' tagging with Dysferlin

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Dysferlin (dysf Zebrafish)

Use

Zebrafish plasmids

Mutation

Please see Depositor Comments

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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p3E DN Kif5a

Plasmid

#105973

Purpose

3' element for Gateway cloning, 5 single nucleotide mutations compared to the reference sequence, but none of them change the amino acidtruncation aa416-1033(end) 1854 bp

Depositor

Caren Norden

Article

Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

Insert

Kif5a (kif5aa Zebrafish)

Expression

Bacterial

Mutation

truncation of aa 416-1033

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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ArpC2_pLib

Plasmid

#173679

Purpose

ArpC2 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

ArpC2 (ARPC2 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_CRKL-2/2

Plasmid

#91364

Purpose

Protein expression and purification of human SH3 domain construct CRKL-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CRKL-2/2 (CRKL Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV10/3xflag-Clock L57E/L113E/W284A

Plasmid

#47343

Purpose

triple mutation used for co-IP

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

3x Flag

Expression

Mammalian

Mutation

L57E/L113E/W284A

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pDONR221 NLRP1 M77T

Plasmid

#166818

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation M77T (Zhong et al., 2016) (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Mutation

M77T

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 A54T

Plasmid

#166815

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation A54T (Zhong et al., 2016) (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Mutation

A54T

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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exon L-firefly

Plasmid

#81006

Purpose

luciferase based mini-gene to report splicing at exon 25 in Drosophila parayltic

Depositor

Richard Baines

Article

Lin et al Brain. 2015 Apr;138(Pt 4):891-901. doi:

Insert

paralytic (para Fly)

Tags

firefly luciferase

Expression

Insect

Mutation

spanning exon 24 to exon 26, termination codon in…

Promoter

P-AC5 (Actin 5C)

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1 hSlp4-a del.SHD

Plasmid

#40067

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a V21A (SYTL4 Human)

Tags

GST

Expression

Bacterial

Mutation

deleted SHD domain (1-143)

Promoter

Tac

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only